Modified vectors for the two-step directional cloning of inverted repeats for RNA interference in Drosophila

BioTechniques

Volumn 44, Issue 3, 2008, Pages 335-339

  Jenny, Andreas ^a,b   Mlodzik, Marek ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PLASMID VECTOR;

DNA REPAIR; DNA REPLICATION; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; LETTER; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CLONING, MOLECULAR; DROSOPHILA MELANOGASTER; GENETIC ENGINEERING; GENETIC VECTORS; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA INTERFERENCE;

EID: 41849101718     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112720     Document Type: Letter

References (13)

1. Sandy, P., A. Ventura, and T. Jacks. 2005. Mammalian RNAi: a practical guide. BioTechniques 39:215-224.
2. Dietzl, G., D. Chen, F. Schnorrer, K.C. Su, Y. Barinova, M. Fellner, B. Gasser, K. Kinsey, et al. 2007. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448:151-156.
3. Kennerdell, J.R. and R.W. Carthew. 2000. Heritable gene silencing in Drosophila using double-stranded RNA. Nat. Biotechnol. 18:896-898.
4. Brand, A.H. and N. Perrimon. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415.
5. Cromie, G.A., C.B. Millar, K.H. Schmidt, and D.R. Leach. 2000. Palindromes as substrates for multiple pathways of recombination in Escherichia coli. Genetics 154:513-522.
6. Lee, Y.S. and R.W. Carthew. 2003. Making a better RNAi vector for Drosophila: use of intron spacers. Methods 30:322-329.
7. Bao, S. and R. Cagan. 2006. Fast cloning inverted repeats for RNA interference. RNA 12:2020-2024.
8. Zhu, X. and D. Stein. 2004. RNAi-mediated inhibition of gene function in the follicle cell layer of the Drosophila ovary. Genesis 40:101-108.
9. Rorth, P. 1998. Gal4 in the Drosophila female germline. Mech. Dev. 78:113-118.
10. Kondo, T., S. Inagaki, K. Yasuda, and Y. Kageyama. 2006. Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system. Genes Genet. Syst. 81:129-134.
11. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
12. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
13. Jenny, A. and M. Mlodzik. 2006. Planar cell polarity signaling: a common mechanism for cellular polarization. Mt. Sinai J. Med. 73:738-750.