The crucial role of chromogranins in storage and exocytosis revealed using chromaffin cells from chromogranin A null mouse

Journal of Neuroscience

Volumn 28, Issue 13, 2008, Pages 3350-3358

  Montesinos, Monica S ^a   Machado, J David ^a   Camacho, Marcial ^a   Diaz, Jesica ^a   Morales, Yezer G ^a   De La Rosa, Diego Alvarez ^a   Carmona, Emilia ^a   Castañeyra, Agustin ^a   Viveros, O Humberto ^a   O'Connor, Daniel T ^b   Mahata, Sushil K ^b   Borges, Ricardo ^a  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Amperometry; Catecholamines; Fusion pore; Neurosecretion; Patch amperometry; Secretory vesicle

Indexed keywords

CHROMOGRANIN A; LEVODOPA;

AMPEROMETRY; ANIMAL CELL; ARTICLE; CATECHOLAMINE RELEASE; CELL CULTURE; CELL ISOLATION; CHROMAFFIN CELL; CONTROLLED STUDY; CULTURE MEDIUM; CYTOSOL; ELECTROCHEMISTRY; EXOCYTOSIS; KINETICS; KNOCKOUT MOUSE; MOUSE; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; SECRETORY VESICLE;

ADRENAL GLANDS; ANIMALS; CATECHOLAMINES; CELLS, CULTURED; CHROMAFFIN CELLS; CHROMOGRANIN A; CHROMOGRANINS; DOPAMINE AGENTS; ELECTROCHEMISTRY; EXOCYTOSIS; LEVODOPA; MEMBRANE FUSION; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; PROBABILITY; TYROSINE 3-MONOOXYGENASE;

EID: 41549134867     PISSN: 02706474     EISSN: 02706474     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.5292-07.2008     Document Type: Article

References (35)

1. Albillos A, Dernick G, Horstmann H, Almers W, Alvarez de Toledo G, Lindau M (1997) The exocytotic event in chromaffin cells revealed by patch amperometry. Nature 389:509 -512.
2. Berneis KH, Goetz U, Da Prada M, Pletscher A (1973) Interaction of aggregated catecholamines and nucleotides with intragranular proteins. Naunyn Schmiedebergs Arch Pharmacol 277:291-296.
3. Borges R, Sala F, Garcia AG (1986) Continuous monitoring of catecholamine release from perfused cat adrenals. J Neurosci Methods 16:289 -300.
4. Borges R, Travis ER, Hochstetler SE, Wightman RM (1997) Effects of external osmotic pressure on vesicular secretion from bovine adrenal medullary cells. J Biol Chem 272:8325- 8331.
5. Borges R, Machado JD, Alonso C, Brioso MA, Gomez JF (2000) Functional role of chromogranins. The intragranular matrix in the last phase of exocytosis. Adv Exp Med Biol 482:69-81.
6. Brekke JF, Kirkeleit J, Lugardon K, Helle KB (2000) Vasostatins. Dilators of bovine resistance arteries. Adv Exp Med Biol 482:239 -246.
7. Camacho M, Machado JD, Montesinos MS, Criado M, Borges R (2006) Intragranular pH rapidly modulates exocytosis in adrenal chromaffin cells. J Neurochem 96:324 -334.
8. Colliver TL, Pyott SJ, Achalabun M, Ewing AG (2000) VMAT-Mediated changes in quantal size and vesicular volume. J Neurosci 20:5276 -5282.
9. Colliver TL, Hess EJ, Ewing AG (2001) Amperometric analysis of exocytosis at chromaffin cells from genetically distinct mice. J Neurosci Methods 105:95-103.
10. Courel M, Rodemer C, Nguyen ST, Pance A, Jackson AP, O'Connor DT, Taupenot L (2006) Secretory granule biogenesis in sympathoadrenal cells: identification of a granulogenic determinant in the secretory prohormone chromogranin A. J Biol Chem 281:38038 -38051.
11. Dernick G, Gong LW, Tabares L, Alvarez de Toledo G, Lindau M (2005) Patch amperometry: high-resolution measurements of single-vesicle fusion and release. Nat Methods 2:699 -708.
12. Fernandez JM, Villalon M, Verdugo P (1991) Reversible condensation of mast cell secretory products in vitro. Biophys J 59:1022-1027.
13. Gleave TL, Beechey RB, Burgoyne RD (2001) Cysteine string protein expression in mammary epithelial cells. Pflugers Archiv Eur J Physiology 441:639-649.
14. Helle KB, Reed RK, Pihl KE, Serck-Hanssen G (1985) Osmotic properties of the chromogranins and relation to osmotic pressure in catecholamine storage granules. Acta Physiol Scand 123:21-33.
15. Hendy GN, Li T, Girard M, Feldstein RC, Mulay S, Desjardins R, Day R, Karaplis AC, Tremblay ML, Canaff L (2006) Targeted ablation of the chromogranin a (Chga) gene: normal neuroendocrine dense-core secretory granules and increased expression of other granins. Mol Endocrinol 20:1935-1947.
16. Huh YH, Jeon SH, Yoo SH (2003) Chromogranin B-induced secretory granule biogenesis: comparison with the similar role of chromogranin A. J Biol Chem 278:40581- 40589.
17. Jankowski JA, Schroeder TJ, Ciolkowski EL, Wightman RM (1993) Temporal Characteristics of quantal secretion of catecholamines from adrenalmedullary cells. J Biol Chem 268:14694 -14700.
18. Jankowski JA, Finnegan JM, Wightman RM (1994) Extracellular ionic composition alters kinetics of vesicular release of catecholamines and quantal size during exocytosis at adrenal-medullary cells. J Neurochem 63:1739 -1747.
19. Kawagoe KT, Zimmerman JB, Wightman RM (1993) Principles of voltammetry and microelectrode surface states. J Neurosci Methods 48:225-240.
20. Kleinbaum D, Kupper K, Muller and Nizam A (1998) Applied regression analysis and other multivariable methods. Pacific Grove, CA: Duxbury.
21. Kopell WN, Westhead EW (1982) Osmotic pressures of solutions of ATP and catecholamines relating to storage in chromaffin granules. J Biol Chem 257:5707-5710.
22. Larsen KE, Schmitz Y, Troyer MD, Mosharov E, Dietrich P, Quazi AZ, Savalle M, Nemani V, Chaudhry FA, Edwards RH, Stefanis L, Sulzer D (2006) Alpha-synuclein overexpression in PC12 and chromaffin cells impairs catecholamine release by interfering with a late step in exocytosis. J Neurosci 26:11915-11922.
23. Machado JD, Segura F, Brioso MA, Borges R (2000) Nitric oxide modulates a late step of exocytosis. J Biol Chem 275:20274 -20279.
24. Mahapatra NR, O'Connor DT, Vaingankar SM, Hikim AP, Mahata M, Ray S, Staite E, Wu H, Gu Y, Dalton N, Kennedy BP, Ziegler MG, Ross J, Mahata SK (2005) Hypertension from targeted ablation of chromogranin A can be rescued by the human ortholog. J Clin Invest 115:1942-1952.
25. Marszalek PE, Farrell B, Verdugo P, Fernandez JM (1997) Kinetics of release of serotonin from isolated secretory granules. I. Amperometric detection of serotonin from electroporated granules. Biophys J 73:1160 -1168.
26. Mosharov EV, Gong LW, Khanna B, Sulzer D, Lindau M (2003) Intracellular patch electrochemistry: regulation of cytosolic catecholamines in chromaffin cells. J Neurosci 23:5835-5845.
27. Nanavati C, Fernandez JM (1993) The secretory granule matrix: a fast-acting smart polymer. Science 259:963-965.
28. Pihel K, Travis ER, Borges R, Wightman RM (1996) Exocytotic release from individual granules exhibits similar properties at mast and chromaffin cells. Biophys J 71:1633-1640.
29. Schroeder TJ, Borges R, Finnegan JM, Pihel K, Amatore C, Wightman RM (1996) Temporally resolved, independent stages of individual exocytotic secretion events. Biophys J 70:1061-1068.
30. Segura F, Brioso MA, Gomez JF, Machado JD, Borges R (2000) Automatic analysis for amperometrical recordings of exocytosis. J Neurosci Methods 103:151-156.
31. Sorensen JB, Nagy G, Varoqueaux F, Nehring RB, Brose N, Wilson MC, Neher E (2003) Differential control of the releasable vesicle pools by SNAP-25 splice variants and SNAP-23. Cell 114:75- 86.
32. Taupenot L, Harper KL, O'Connor DT (2003) The chromograninsecretogranin family. N Engl J Med 348:1134 -1149.
33. Videen JS, Mezger MS, Chang YM, O'Connor DT (1992) Calcium and catecholamine interactions with adrenal chromogranins. Comparison of driving forces in binding and aggregation. J Biol Chem 267:3066 -3073.
34. Wen G, Mahata SK, Cadman P, Mahata M, Ghosh S, Mahapatra NR, Rao F, Stridsberg M, Smith DW, Mahboubi P, Schork NJ, O'Connor DT, Hamilton BA (2004) Both rare and common polymorphisms contribute functional variation at CHGA, a regulator of catecholamine physiology. Am J Hum Genet 74:197-207.
35. Winkler H, Laslop A, Leitner B, Weiss C (1998) The secretory cocktail of adrenergic large dense-core vesicles: the functional role of the chromogranins. Adv Pharmacol 42:257-259.