Optimization of the porous structure and polarity of polymethacrylate-based monolithc capillary columns for the LC-MS separation of enzymatic digests

Journal of Separation Science

Volumn 30, Issue 17, 2007, Pages 2814-2820

  Eeltink, Sebastian ^a,b   Geiser, Laurent ^a,b   Svec, Frantisek ^b   Fréchet, Jean M J ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Column technology; High performance liquid chromatography; Monolithic stationary phase; Peptides; Temperature

Indexed keywords

CAPILLARY COLUMNS; ENZYMATIC DIGESTION; MONOLITHIC STATIONARY PHASE;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OPTIMIZATION; PEPTIDES; POLYMERIZATION; POROSITY; PRESSURE DROP; SEPARATION; TEMPERATURE;

POLYMETHYL METHACRYLATES;

COPOLYMER; CYTOCHROME C; POLY(BUTYL METHACRYLATE CO ETHYLENE DIMETHACRYLATE); POLY(LAURYL METHACRYLATE CO ETHYLENE DIMETHACRYLATE); POLY(OCTADECYL METHACRYLATE CO ETHYLENE DIMETHACRYLATE); POLYMETHACRYLIC ACID; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH TEMPERATURE PROCEDURES; MASS SPECTROMETRY; PHYSICAL CHEMISTRY; POLYMERIZATION; POROSITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; REACTION TIME; SEPARATION TECHNIQUE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROMES C; MASS SPECTROMETRY; METHACRYLATES; MICROSCOPY, ELECTRON, SCANNING; PARTICLE SIZE; PEPTIDE FRAGMENTS; POLYMETHACRYLIC ACIDS; POROSITY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOLVENTS; SURFACE PROPERTIES; TEMPERATURE; TIME FACTORS; TRYPSIN;

EID: 36949032551     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200700185     Document Type: Article

References (34)

1. van der Greef, J., Stroobant, P., van der Heijden, R., Curr. Opinion. Chem. Biol. 2004, 8, 559-565.
2. Lopez-Ferrer, D., Canas, B., Vazquez, J., Lodeiro, C., Rial-Otero, R., Moura, I., Capelo, J. L., Trends Anal. Chem. 2006, 25, 996-1005.
3. O'Farell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
4. Righetti, P. G., Electrophoresis 2004, 25, 2111-2127.
5. Mitulovic, G., Stingl, C., Smoluch, M., Swart, R., Chervet, J. P., Steinmacher, I., Gerner, C., Mechtler, K., Proteomics 2004, 4, 2545-2557.
6. Svec, F., Fréchet, J. M. J., Anal. Chem. 1992, 54, 820-822.
7. Hjertén, S., Liao, J. L., Zhang, R., J. Chromatogr. 1989, 473, 273-275.
8. Palm, A., Novotny, M. V., Anal. Chem. 1997, 69, 4499-4507.
9. Gusev, I., Huang, X., Horvath, C., J. Chromatogr. A 1999, 855, 273-290.
10. Sondergeld, L. J., Bush, M. E., Bellinger, A., Bushey, M. M., J. Chromatogr. A 2003, 1004, 155-165.
11. Svec, F., Tennikova, T. B., Deyl, Z. (Eds.), Monolithic Materials: Preparation, Properties, and Applications, Elsevier, Amsterdam 2003.
12. Sakka, S., Adachi, T., J. Mater. Sci. 1990, 25, 3408-3414.
13. Minakuchi, H., Nakanishi, K., Soga, N., Ishizuka, N., Tanaka, N., Anal. Chem. 1996, 68, 3498-3501.
14. Nakanishi, K., Minakuchi, H., Soga, N., Tanaka, N., J. Sol-Gel Sci. Technol. 1997, 8, 547-552.
15. Yu, C., Svec, F., Fréchet, J. M. J., Electrophoresis 2000, 21, 120-127.
16. Okanda, F. M., El Rassi, M., Electrophoresis 2005, 26, 1988-1995.
17. Yu, C., Xu, M. C., Svec, F., Fréchet, J. M. J., J. Polym. Sci., Polym. Chem. 2002, 40, 755-769.
18. Eeltink, S., Herrero-Martinez, J. M., Rozing, G. P., Schoenmakers, P. J., Kok, W. T., Anal. Chem. 2005, 77, 7342-7347.
19. Gzil, P., De Smet, J., Desmet, G., J. Sep. Sci. 2006, 29, 1675-1685.
20. Eeltink, S., Svec, F., Electrophoresis 2007, 28, 137-147.
21. Kirkland, J. J., DeStefano, J. J., J. Chromatogr. A 2006, 1126, 50-57.
22. Luo, Q. Z., Tang, K. Q., Yang, F., Elias, A., Shen, Y. F., Moore, R. J., Zhao, R., Hixson, K. K., Rossie, S. S., Smith, R. D., J. Proteome Res. 2006, 5, 1091-1097.
23. Rieux, L., Lubda, D., Niederlander, H. A. G., Verpoorte, E., Bischoff, R., J. Chromatogr. A 2006, 1120, 165-172.
24. Yue, G., Luo, Q., Zhang, J., Wu, S. L., Karger, B. L., Anal. Chem. 2007, 79, 938-946.
25. Premstaller, A., Oefner, P. J., Oberacher, H., Huber, C. G., Anal. Chem. 2002, 74, 4688-4693.
26. Trojer, L., Lubbad, S. H., Bisjak, C. P., Bonn, G. K., J. Chromatogr. A 2006, 1117, 56-66.
27. Svec, F., Huber, C. G., Anal. Chem. 2006, 78, 2100-2107.
28. Svec, F., J. Sep. Sci. 2004, 27, 1419-1430.
29. Svec, F., J. Sep. Sci. 2004, 27, 747-766.
30. Geiser, L., Eeltink, S., Svec, F., Fréchet, J. M. J., J. Chromatogr. A 2007, 1140, 140-146.
31. Wang, Q, Svec, F., Fréchet, J. M. J., J. Chromatogr. A 1994, 669, 230-235.
32. Yu, C., Davey, M. H., Svec, F., Fréchet, J. M. J., Anal. Chem. 2001, 73, 5088-5096.
33. Viklund, C., Svec, F., Fréchet, J. M. J., Irgum, K., Chem. Mater. 1996, 8, 744-750.
34. Peters, E. C., Petro, M., Svec, F., Fréchet, J. M. J., Anal. Chem. 1998, 70, 2288-2295.