A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases

Analytical Biochemistry

Volumn 376, Issue 1, 2008, Pages 151-153

  Marcon, Lionel ^a   Kozak, Darby ^a   Battersby, Bronwyn J ^a   Chappell, Keith J ^a   Fairlie, David P ^a   Young, Paul ^a   Trau, Matt ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; PEPTIDES; SILICA; SOLS; SYNTHESIS (CHEMICAL); VIRUSES;

FLOW-CYTOMETRIC ANALYSIS; OPTICAL SIGNATURES; PEPTIDE SUBSTRATES; SILICA PARTICLES; SIMULTANEOUS IDENTIFICATION; SOLID PHASE PEPTIDE SYNTHESIS; SUBSTRATE POSITIONS; SUBSTRATE RECOGNITION;

SUBSTRATES;

PROTEINASE;

ARTICLE; DENGUE VIRUS; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; WEST NILE FLAVIVIRUS;

DENGUE VIRUS; WEST NILE VIRUS;

EID: 41449108064     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.01.034     Document Type: Article

References (11)

1. Mackenzie J.S., Gubler D.J., and Petersen L.R. Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat. Med. 10 (2004) S98-S109
2. Chambers T.J., Weir R.C., Grakoui A., McCourt D.W., Bazan J.F., Fletterick R.J., and Rice C.M. Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proc. Natl. Acad. Sci. USA 87 (1990) 8898-8902
3. Chappell K.J., Stoermer M.J., Fairlie D.P., and Young P.R. Insights to substrate binding and processing by West Nile Virus NS3 protease through combined modeling, protease mutagenesis, and kinetic studies. J. Biol. Chem. 281 (2006) 38448-38458
4. Li J., Lim S.P., Beer D., Patel V., Wen D., Tumanut C., Tully D.C., Williams J.A., Jiricek J., Priestle J.P., Harris J.L., and Vasudevan S.G. Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries. J. Biol. Chem. 280 (2005) 28766-28774
5. Miller C.R., Vogel R., Surawski P.P., Corrie S.R., Ruhmann A., and Trau M. Biomolecular screening with novel organosilica microspheres. Chem. Commun. (2005) 4783-4785
6. Nielsen T.E., and Meldal M. Solid-phase intramolecular N-acyliminium Pictet-Spengler reactions as crossroads to scaffold diversity. J. Org. Chem. 69 (2004) 3765-3773
7. Mellor S. Fmoc Solid Phase Peptide Synthesis: A Practical Approach (2000), Oxford University Press, Oxford pp. 147-148
8. Leung D., Schroder K., White H., Fang N.X., Stoermer M.J., Abbenante G., Martin J.L., Young P.R., and Fairlie D.P. Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors. J. Biol. Chem. 276 (2001) 45762-45771
9. St-Pierre Y., Desrosiers M., Tremblay P., Esteve P.O., and Opdenakker G. Flow cytometric analysis of gelatinase B (MMP-9) activity using immobilized fluorescent substrate on microspheres. Cytometry 25 (1996) 374-380
10. Saunders M.J., Kim H., Woods T.A., Nolan J.P., Sklar L.A., Edwards B.S., and Graves S.W. Microsphere-based protease assays and screening application for lethal factor and factor Xa. Cytometry A 69 (2006) 342-352
11. Battersby B.J., and Trau M. Optically encoded particles and their applications in multiplexed biomedical assays. Aust. J. Chem. 60 (2007) 343-353