A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts

Analytical Biochemistry

Volumn 376, Issue 1, 2008, Pages 137-143

  Su, Xiaodi ^a   Neo, Siew Jun ^a   Peh, Wendy Y X ^a   Thomsen, Jane S ^b  

^a INSTITUTE OF MATERIALS RESEARCH AND ENGINEERING   (Singapore)

^b GENOME INSTITUTE OF SINGAPORE   (Singapore)

Author keywords

Estrogen receptors; Estrogen response elements; Nuclear extract; Protein DNA interaction; SPR

Indexed keywords

ANTIBODIES; CHEMICAL DETECTION; DNA; DNA SEQUENCES; PLASMONS; RECOMBINANT PROTEINS;

DETECTION SENSITIVITY; ESTROGEN RECEPTOR; ESTROGEN RESPONSE ELEMENTS; MCF-7 BREAST CANCER CELLS; NUCLEAR EXTRACTS; PROTEIN-DNA INTERACTIONS; SECONDARY ANTIBODIES; SURFACE PLASMON RESONANCE SPECTROSCOPY;

SURFACE PLASMON RESONANCE;

ANTIBODY; DNA; ESTROGEN RECEPTOR ALPHA;

ANTIGEN BINDING; ARTICLE; CELL NUCLEUS; CELL STRAIN MCF 7; CONTROLLED STUDY; ESTROGEN RESPONSIVE ELEMENT; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; SENSITIVITY AND SPECIFICITY; SURFACE PLASMON RESONANCE;

CAPRA HIRCUS; ORYCTOLAGUS CUNICULUS;

EID: 41449085504     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.02.002     Document Type: Article

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