Salt-inducible multidrug efflux pump protein in the moderately halophilic bacterium Chromohalobacter sp.

Applied and Environmental Microbiology

Volumn 70, Issue 8, 2004, Pages 4424-4431

  Tokunaga, Hiroko ^a   Mitsuo, Kenjiro ^a   Ichinose, Sachiyo ^b   Omori, Akira ^b   Ventosa, Antonio ^d   Nakae, Taiji ^c   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b MITSUBISHI KAGAKU INSTITUTE OF LIFE SCIENCES   (Japan)

^c TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d UNIVERSITY OF SEVILLE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BIOLOGICAL MEMBRANES; DRUG PRODUCTS; DYES; ESCHERICHIA COLI; GENES; MICROBIOLOGY; ORGANIC SOLVENTS; POSITIVE IONS; PROTEINS; TOXIC MATERIALS;

EFFLUX PUMPS; HALOPHILIC BACTERIA; MULTIDRUG RESISTANCE; MUTANT;

BACTERIA;

CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; CEFSULODIN; CHLORAMPHENICOL; ETHIDIUM BROMIDE; HEPTANE; HEXANE; MULTIDRUG EFFLUX PUMP PROTEIN; OFLOXACIN; ORGANIC SOLVENT; OUTER MEMBRANE PROTEIN; SODIUM CHLORIDE; TETRACYCLINE; UNCLASSIFIED DRUG;

MICROBIOLOGY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL MEMBRANE; BACTERIUM CULTURE; BACTERIUM MUTANT; CELLULAR DISTRIBUTION; CHROMOHALOBACTER; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE DISRUPTION; GENE IDENTIFICATION; GENETIC CODE; HALOPHILIC BACTERIUM; HRDC GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SALT TOLERANCE; TOLC GENE; WILD TYPE;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL OUTER MEMBRANE PROTEINS; CLONING, MOLECULAR; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; HALOMONADACEAE; MOLECULAR SEQUENCE DATA; MUTATION; OSMOLAR CONCENTRATION; SEQUENCE ANALYSIS, DNA; SODIUM CHLORIDE; SOLVENTS;

BACTERIA (MICROORGANISMS); CHROMOHALOBACTER; CHROMOHALOBACTER SP.; ESCHERICHIA COLI;

EID: 4143117854     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.8.4424-4431.2004     Document Type: Article

References (38)

1. Adewoye, L., A. Sutherland, R. Srikumar, and K. Poole. 2002. The mexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: characterization of mutations compromising activity. J. Bacteriol. 184:4308-4312.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology. John Wiley and Sons, New York, N.Y.
3. Avila-Sakar, A. J., S. Misaghi, E. M. Wilson-Kubalek, K. H. Downing, H. Zgurskaya, H. Nikaido, and E. Nogales. 2001. Lipid-layer crystallization and preliminary three-dimensional structural analysis of AcrA, the periplasmic component of a bacterial multidrug efflux pump. J. Struct. Biol. 136:81-88..
4. Coronado, M.-J., C. Vargas, H. J. Kunte, E. A. Galinski, A. Ventosa, and J. J. Nieto. 1995. Influence of salt concentration on the susceptibility of moderately halophilic bacteria to antimicrobials and its potential use for genetic transfer studies. Curr. Microbiol. 31:365-371.
5. Fukuda, H., M. Hosaka, K. Hirai, and S. Iyobe. 1990. New norfloxacin resistance gene in Pseudomonas aeruginosa PAO. Antimicrob. Agents Chemother. 34:1757-1761.
6. Guan, L., M. Ehrmann, H. Yoneyama, and T. Nakae. 1999. Membrane topology of the xenobiotic-exporting subunit, MexB, of the MexA,B-OprM extrusion pump in Pseudomonas aeruginosa. J. Biol. Chem. 274:10517-10522.
7. Ikezono, T., A. Omori, S. Ichinose, R. Pawankar, A. Watanabe, and T. Yagi. 2001. Identification of the protein product of the Coch gene (hereditary deafness gene) as the major component of bovine inner ear protein. Biochim. Biophys. Acta 1535:258-265.
8. Inoue, A., and K. Horikoshi. 1989. A Pseudomonas thrives in high concentration of toluene. Nature 338:264-266.
9. Karkhanis, Y. D., J. Y. Zeltner, J. J. Jackson, and D. J. Carlo. 1978. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria. Anal. Biochem. 85:595-601.
10. Komatsubara, M., A. Abe, R. Okumura, S. Miyauchi, and N. Kamo. 1996. Doxorubicin-efflux pump in Haloferax volcanii, is energized by ATP. FEMS Microbiol. Lett. 139:83-88.
11. Koronakis, V., A. Sharff, E. Koronakis, B. Luisi, and C. Hughes. 2000. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914-919.
12. Kushner, D. J. 1985. The Halobacteriaceae, p. 171-214. In C. R. Woese and R. S. Wolfe (ed.), The bacteria, vol. 8. Academic Press Inc., Orlando, Fla.
13. Kushner, D. J., and M. Kamekura. 1988. Physiology of halophilic eubacteria, p. 109-140. In F. Rodriguez-Valera (ed.), Halophilic bacteria, vol. 1. CRC Press, Boca Raton, Fla.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
15. Lei, Y., K. Sato, and T. Nakae. 1991. Ofloxacin-resistant Pseudomonas aeruginosa mutants with elevated drug extrusion across the inner membrane. Biochem. Biophys. Res. Commun. 178:1043-1048.
16. Madern, D., C. Pfister, and G. Zaccai. 1995. Mutation at a single acidic amino acid enhances the halophilic behaviour of malate dehydrogenase from Haloarcula marismortui in physiological salts. Eur. J. Biochem. 230:1088-1095.
17. Martin, R. G., W. K. Gillette, S. Rhee, and J. L. Rosner. 1999. Structural requirements for mar box function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter. Mol. Microbiol. 34:431-441.
18. Masuda, N., and S. Ohya. 1992. Cross-resistance to meropenem, cephems, and quinolones in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 36:1847-1851.
19. Miyauchi, S., M. Komatsubara, and N. Kamo. 1992. In archaebacteria, there is a doxorubicin efflux pump similar to mammalian P-glycoprotein. Biochim. Biophys. Acta 1110:144-150.
20. +-driven multidrug efflux pump. J. Bacteriol. 182:6694-6697.
21. Morshed, S. R. M., Y. Lei, H. Yoneyama, and T. Nakae. 1995. Expression of genes associated with antibiotic extrusion in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 210:356-362.
22. Murakami, S., R. Nakajima, E. Yamashita, and A. Yamaguchi. 2002. Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419:587-593.
23. Nietro, J. J., R. Fernandez-Castillo, M. T. Garcia, E. Mellado, and A. Ventosa. 1993. Survey of antimicrobial susceptibility of moderately halophilic eubacteria and extremely halophilic aerobic archaeobacteria; utilization of antimicrobial resistance as a genetic marker. Syst. Appl. Microbiol. 16:352-360.
24. Nieto, J. J., R. Fernandez-Castillo, M. C. Marquez, A. Ventosa, E. Quesada, and F. Ruiz-Berraquero. 1989. Survey of metal tolerance in moderately halophilic eubacteria. Appl. Environ. Microbiol. 55:2385-2390.
25. Nikaido, H. 1996. Multidrug efflux pumps of gram-negative bacteria. J. Bacteriol. 178:5853-5859.
26. Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
27. Poole, K. 2001. Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms. J. Mol. Microbiol. Biotechnol. 3:255-264.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989, Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
29. Tokunaga, H., M. Ishibashi, T. Arakawa, and M. Tokunaga. 2004. Highly efficient renaturation of β-lactamase isolated from moderately halophilic bacteria. FEES Lett. 558:7-12.
30. Tokunaga, M., A. Kawamura, S. Yonekyu, M. Kishida, and F. Hishinuma. 1993. Secretion of mouse α-amylase from fission yeast Schizosaccharomyces pombe: presence of chymostatin-sensitive protease activity in the culture medium. Yeast 9:379-387.
31. Tsai, C.-M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
32. Vargas, C., M. J. Coronado, A. Ventosa, and J. J. Nieto. 1997. Host range, stability and compatibility of broad host-range plasmid and a shuttle vector in moderately halophilic bacteria. Evidence of intrageneric and intergeneric conjugation in moderate halophiles. Syst. Appl. Microbiol. 20:173-181.
33. Vargas, C., R. Fernandez-Castillo, D. Canovas, A. Ventosa, and J. J. Nieto. 1995. Isolation of cryptic plasmid from moderately halophilic eubacteria of the genus Halomonas. Characterization of a small plasmid from H. elongata and its use for shuttle vector construction. Mol. Gen. Genet. 246:411-418.
34. Vargas, C., G. Tegos, C. Drainas, A. Ventosa, and J. J. Nieto. 1999. Analysis of the replication region of the cryptic plasmid pHE1 from the moderate halophile Halomonas elongata. Mol. Gen. Genet. 261:851-861.
35. Ventosa, A., J. J. Nieto, and A. Oren. 1998. Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62:504-544.
36. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharide. Extraction with phenol water and further applications of the procedure, p. 83-91. In R. L. Whistler (ed.), Methods in carbohydrate chemistry, vol. 5. Academic Press, New York, N. Y.
37. Yu, E. W., G. McDermott, H. I. Zgurskaya, H. Nikaido, and D. E. Koshland, Jr. 2003. Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Science 300:976-980.
38. Zgurskaya, H. I., and H. Nikaido. 2000. Multidrug resistance mechanisms: drug efflux across two membranes. Mol. Microbiol. 37:219-225.