Identification of an Na+-dependent transporter associated with saxitoxin-producing strains of the cyanobacterium Anabaena circinalis

Applied and Environmental Microbiology

Volumn 70, Issue 8, 2004, Pages 4711-4719

  Pomati, Francesco ^a   Burns, Brendan P ^a   Neilan, Brett A ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DERIVATIVES; DNA; GENES; GROUNDWATER; HEALTH RISKS; METABOLISM; PROTEINS; TOXIC MATERIALS;

FRESHWATERS; GENOMIC SCREENING; SAXITOXIN (STX);

BACTERIA;

SAXITOXIN;

MICROBIOLOGY;

ANABAENA; ANIMAL CELL; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CYANOBACTERIUM; DNA MICROARRAY; GENE STRUCTURE; MOLECULAR PROBE; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN FAMILY; SODIUM TRANSPORT; STRAIN DIFFERENCE; SUPPRESSION SUBTRACTIVE HYBRIDIZATION;

AMINO ACID SEQUENCE; ANABAENA; BACTERIAL PROTEINS; GENE LIBRARY; GENOME, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHYLOGENY; POLYMORPHISM, GENETIC; REPETITIVE SEQUENCES, NUCLEIC ACID; SAXITOXIN; SEQUENCE ANALYSIS, DNA; SODIUM; SPECIES SPECIFICITY;

ANABAENA; ANABAENA CIRCINALIS; ANIMALIA; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); CYANOBACTERIA; NEGIBACTERIA;

EID: 4143102778     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.8.4711-4719.2004     Document Type: Article

References (44)

1. Akopyants, N. S., A. Fradkov, L. Diatchenko, J. E. Hill, P. D. Siebert, S. A. Lukyanov, E. D. Sverdlov, and D. E. Berg. 1998. PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc. Natl. Acad. Sci. USA 95:13108-13113.
2. Alam, M., M. Ikawa, J. J. Sasner, Jr., and P. J. Sawyer. 1973. Purification of Aphanizomenon flos-aquae toxin and its chemical and physiological properties. Toxicon 11:65-72.
3. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
4. Beltran, E. C. 2001. Taxonomy and molecular genetics of the saxitoxin producing cyanobacterium Anabaena circinalis. Ph.D. thesis. School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia.
5. Beltran, E. C., and B. A. Neilan. 2000. Geographical segregation of the neurotoxin-producing cyanobacterium Anabaena circinalis. Appl. Environ. Microbiol. 66:4468-4474.
6. Bhaya, D., D. Vaulot, P. Amin, A. W. Takahashi, and A. R. Grossman. 2000. Isolation of regulated genes of the cyanobacterium Synechocystis sp. strain PCC6803 by differential display. J. Bacteriol. 182:5692-5699.
7. Bowling, L. C., and P. D. Baker. 1996. Major cyanobacterial bloom in the Barwon-Darling River, Australia, in 1991, and underlying limnological conditions. Mar. Freshwater Res. 47:643-657.
8. Carmichael, W. W., W. R. Evans, Q. Q. Yin, P. Bell, and E. Moczydlowsky. 1997. Evidence of paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei (Farlow ex Gomont) comb. nov. Appl. Environ. Microbiol. 63:3104-3110.
9. Catterall, W. A. 1980. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Annu. Rev. Pharmacol. Toxicol. 20:15-43.
10. Gallacher, S., and E. A. Smith. 1999. Bacteria and paralytic shellfish toxins. Protist 150:245-255.
11. Gupta, A., A. P. Morby, J. S. Turner, B. A. Whitton, and N. J. Robinson. 1993. Deletion within the metallothionein locus of cadmium tolerant Synechococcus PCC6301 involving a highly iterated palindrome (Hip1). Mol. Microbiol. 5:825-834.
12. Harada, T., Y. Oshima, and T. Yasumoto. 1982. Structure of two paralytic shellfish toxins, Gonyautoxins V and VI, isolated from a tropical dinoflagellate, Pyrodinium bahamense var. compressa. Agric. Biol. Chem. 46:1861-1864.
13. Harakava, R., and D. W. Gabriel. 2003. Genetic differences between two strains of Xylella fastidiosa revealed by suppression subtractive hybridization. Appl. Environ. Microbiol. 69:1315-1319.
14. Hawkins, P. R., N. R. Chandrasena, G. J. Jones, A. R. Humpage, and I. R. Falconer. 1997. Isolation and toxicity of Cylindrospermopsis raciborskii from an ornamental lake. Toxicon 35:341-346.
15. Humpage, A. R., J. Rositano, A. Bretag, R. Brown, P. Baker, B. C. Nicholson, and D. A. Steffensen. 1994. Paralytic shellfish poisons from Australian cyanobacterial blooms. Aust. J. Mar. Freshwater Res. 45:761-771.
16. + and in pH homeostasis. J. Bacteriol. 181:2394-2402.
17. + and alkali but not cholate resistance. J. Bacteriol. 182:5663-5670.
18. Jukes, T. H., and C. R. Cantor. 1969. Evolution of protein molecules, p. 21-132. In H. N. Munro (ed.), Mammalian protein metabolism, vol. 3. Academic Press Inc., New York, N.Y.
19. Kaebernick, M. 2001. Regulation and function of microcystin production in Microcystis aeruginosa. Ph.D. thesis. School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia.
20. Kao, C. Y. 1993. Paralytic shellfish poisoning, p. 75. In E. R. Falconer (ed.), Algal toxins in seafood and drinking water. Academic Press, London, United Kingdom.
21. Lagos, N., H. Onodera, P. A. Zagatto, D. Andrinolo, S. M. F. Q. Azevedo, and Y. Oshima. 1999. The first evidence of paralytic shellfish toxins in the freshwater cyanobacterium Cylindrospermopsis raciborskii, isolated from Brazil. Toxicon 37:1359-1373.
22. Llewellyn, L. E., A. P. Negri, J. Doyle, P. D. Baker, E. C. Beltran, and B. A. Neilan. 2001. Radioreceptor assays for sensitive detection and quantitation of saxitoxin and its analogues from strains of the freshwater cyanobacterium, Anabaena circinalis. Environ. Sci. Technol. 35:1445-1451.
23. Mavrodi, D. V., O. V. Mavrodi, B. B. McSpadden-Gardener, B. B. Landa, D. M. Weller, and L. S. Thomashow. 2002. Identification of differences in genome content among ph/D-positive Pseudomonas fluorescens strains by using PCR-based subtractive hybridization. Appl. Environ. Microbiol. 68: 5170-5176.
24. Neilan, B. A., M. L. Saker, J. Fastner, A. Torokne, and B. P. Burns. 2003. Phylogeography of the invasive cyanobacterium Cylindrospermopsis raciborskii. Mol. Ecol. 12:133-140.
25. Nesbø, C. L., K. E. Nelson, and W. F. Doolittle. 2002. Suppressive subtractive hybridization detects extensive genomic diversity in Thermotoga maritima. J. Bacteriol. 184:4475-4488.
26. Oshima, Y., M. Hasegawa, T. Yasumoto, G. Hallegaeff, and S. Blackburn. 1987. Dinoflagellate Gimnodium catenatum as the source of paralytic shellfish toxins in Tasmanian shellfish. Toxicon 25:1105-1111.
27. Pomati, F., and B. A. Neilan. 2004. PCR-based positive hybridization to detect genomic diversity associated with bacterial secondary metabolism. Nucleic Acid Res. 32:e7. [Online.]
28. Pomati, F., B. A. Neilan, G. Manarolla, T. Suzuki, and C. Rossetti. 2003. Enhancement of intracellular saxitoxin accumulation by lidocatne hydrochloride in the cyanobacterium Cylindrospermopsis raciborskii T3 (Nostocales). J. Phycol. 39:535-542.
29. + fluxes: a prokaryote-based STX bioassay. Appl. Environ. Microbiol. 69:7371-7376.
30. + levels and saxitoxin production in Cylindrospermopsis raciborskii T3. Microbiology 150:455-461.
31. Pomati, F., S. Sacchi, C. Rossetti, S. Giovannardi, H. Onodera, Y. Oshima, and B. A. Neilan. 2000. The freshwater cyanobacterium Planktothrix sp. FP1: molecular identification and detection of paralytic shellfish poisoning toxins. J. Phycol. 36:553-562.
32. Robinson, N. J., P. J. Robinson, A. Gupta, A. J. Bleasby, B. A. Whitton, and A. P. Morby. 1995. Singular overrepresentation of an octameric palindrome, HIP1, in DNA from many cyanobacteria. Nucleic Acids Res. 23:729-735.
33. Saitou, N., and M. Nei. 1987. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
34. Saker, M. L., and B. A. Neilan. 2001. Varied diazotrophies, morphologies, and toxicities of genetically similar isolates of Cylindrospermopsis raciborskii (Nostocales, Cyanophyceae) from northern Australia. Appl. Environ. Microbiol. 67:1839-1845.
35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
36. Shimizu, Y. 1977. Chemistry and distribution of deleterious dinoflagellate toxins, p. 261-269. In D. J. Faulkner and W. H. Fenical (ed.), Marine Natural Products Chemistry. Plenum, New York, N.Y.
37. Shimizu, Y. 1993. Microalgal metabolites. Chem. Rev. 93:1685-1698.
38. Sivonen, K., and G. Jones. 1999. Cyanobacterial toxins, p. 41-111. In I. Chorus and J. Bartram (ed.), Toxic cyanobacteria in water. E & FN Spon Publishers, London, United Kingdom.
39. Smith, J. K., J. D. Parry, J. G. Day, and R. J. Smith. 1998. A PCR technique based on the Hip1 interspersed repetitive sequence distinguishes cyanobacterial species and strains. Microbiology 144:2791-2801.
40. Taroncher-Oldenburg, G., and D. M. Anderson. 2000. Identification and characterization of three differentially expressed genes, encoding S-adenosylhomocysteine hydrolase, methionine aminopeptidase, and a histone-like protein, in the toxic dinoflagellate Alexandrium fundyense. Appl. Environ. Microbiol. 66:2105-2112.
41. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24:4876-4882.
42. Tillett, D., and B. A. Neilan. 2000. Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol. 35:1-8.
43. Zheng, W., T. Song, X. Bao, B. Bergman, and U. Rasmussen. 2002. High cyanobacterial diversity in coralloid roots of cycads revealed by PCR finger-printing. FEMS Microbiol. Ecol. 40:215-222.
44. Zingone, A., and H. O. Enevoldsen. 2000. The diversity of harmful algal blooms: a challenge for science and management. Ocean & Coastal Management 43:725-748.