Quantitative real-time PCR - A question of normalization;Quantitative real-time PCR - Eine frage der normalisierung

BioSpektrum

Volumn 10, Issue 1, 2004, Pages 99-102

  Wiedemann, Christine ^a   Schulz, Heidi L ^a   Weber, Bernhard H F ^a,b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; DNA TEMPLATE; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; SHORT SURVEY;

EID: 4143052254     PISSN: 09470867     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Short Survey

References (7)

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2. Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., and Speleman, F. (2002): Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3: research 0034.1-0034.11 (http://genomebiology.com/2002/ 3/7/research/0034.1)
3. Ramakers, C., Ruijter, J.M., Deprez, R.H., Moorman, A.F. (2003): Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339: 62-66
4. Liu, W., and Saint, D.A. (2002): Validation of a quantitative method for real time PCR kinetics. Biochem. Biophys. Res. Commun. 294: 347-353
5. Rasmussen, R. (2001): Quantification on the Light-Cycler; In: Meuer, S., Wittwer, C. and Nakagawara, K., (eds), Rapid Cycle Real-time PCR, Methods and Applications. Springer Press, Heidelberg, pp 21-34
6. Tricarico, C., Pinzani, P., Bianchi, S., Paglierani, M., Distante, V., Pazzagli, M., Bustin, S.A., and Orlando, C. (2002): Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309: 293-300
7. Thellin, O., Zorzi, W., Lakaye, B., De Borman, B., Coumans, B., Hennen, G., Grisar, T., Igout, A., and Heinen, E. (1999): Housekeeping genes as internal standards: use and limits, J. Biotechnol. 75: 291-295