L-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line

Acta Oto-Laryngologica

Volumn 128, Issue 4, 2008, Pages 440-445

  Low, Wong Kein ^a   Sun, Li ^a   Tan, Michelle G K ^a   Chua, Alvin W C ^a   Wang, De Yun ^b  

^a SINGAPORE GENERAL HOSPITAL   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Antioxidant; Deafness; Hair cell; Hearing loss; Hearing preservation; Inner ear; Intratympanic; Ototoxicity; Radiation; Radiotherapy

Indexed keywords

ACETYLCYSTEINE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CANCER RADIOTHERAPY; CELL CULTURE; CELL LINE; CELL VIABILITY; COCHLEA DUCT; DRUG EFFICACY; FLOW CYTOMETRY; FLUORESCENCE; HEAD AND NECK TUMOR; MOUSE; NICK END LABELING; NONHUMAN; OTOTOXICITY; PERCEPTION DEAFNESS; PRIORITY JOURNAL; RADIATION INDUCED APOPTOSIS;

ACETYLCYSTEINE; ANIMALS; APOPTOSIS; CELL LINE; COCHLEA; FLOW CYTOMETRY; FREE RADICAL SCAVENGERS; HEARING LOSS, SENSORINEURAL; IN SITU NICK-END LABELING; MICE; MICE, TRANSGENIC; RADIATION INJURIES, EXPERIMENTAL; REACTIVE OXYGEN SPECIES;

EID: 41349105979     PISSN: 00016489     EISSN: 16512553     Source Type: Journal    
DOI: 10.1080/00016480701762490     Document Type: Article

References (20)

1. Seidman MD, Vivek P. Intratympanic treatment of hearing loss with novel and traditional agents. Otolaryngol Clin North Am 2004;37:973-90.
2. Cheng AG, Cunningham LL, Rubel EW. Mechanisms of hair cell death and protection. Curr Opin Otolaryngol Head Neck Surg 2005;13:343-8.
3. Low WK, Tan MGK, Li S, Chua AWC, Goh LK, Wang DY. Dose-dependent radiation-induced apoptosis in a cochlear cell-line. Apoptosis 2006;11:2127-36.
4. Feghali JG, Liu W, Van De Water TR. L-n-acetyl-cysteine protection against cisplatin-induced auditory neuronal and hair cell toxicity. Laryngoscope 2001;111:1147-55.
5. De Flora S, Izzotti A, D'Agostini F, Balansky RM. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related endpoints. Carcinogenesis 2001;22:999-1013.
6. Cotgreave IA. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. Adv Pharmacol 1997;38:205-27.
7. Kopke R, Bielefeld E, Liu J, Zheng J, Jackson R, Henderson D, et al. Prevention of impulse noise-induced hearing loss with antioxidants. Acta Otolaryngol (Stockh) 2005;125:235-43.
8. Klein M, Koedel U, Pfister HW, Kastenbauer S. Meningitis-associated hearing loss: protection by adjunctive antioxidant therapy. Ann Neurol 2003;54:451-8.
9. Bertolaso L, Martini A, Bindini D, Lanzoni I, Parmeggiani A, Vitali C, et al. Apoptosis in the OC-k3 immortalized cell line treated with different agents. Audiology 2001;40:327-35.
10. Kalinec F, Kalinec G, Boukhvalova M, Kachar B. Establishment and characterization of conditionally immortalized organ of corti cell lines. Cell Biol Int 1999;23:175-84.
11. Devarajan P, Savoca M, Castaneda MP, Park MS, Esteban-Cruciani N, Kalinec G, et al. Cisplatin-induced apoptosis in auditory cells: role of death receptor and mitochondrial pathways. Hear Res 2002;174:45-54.
12. Tan DTH, Liu YP, Sun L. Flow cytometer measurements of DNA content in primary and recurrent ptergia. Inv Ophothol Vis Sci 2000;41:1684-6.
13. Ogawa Y, Kobayashi T, Nishioka A, Kariya S, Hamasato S, Seguchi H, et al. Radiation-induced reactive oxygen species formation prior to oxidative DNA damage in human peripheral T cells. Int J Mol Med 2003;11:149-52.
14. Jayalakshmi K, Sairam M, Singh SB, Sharma SK, Ilavazhagan G, Banerjee PK. Neuroprotective effect of N-acetyl cysteine on hypoxia-induced oxidative stress in primary hippocampal culture. Brain Res 2005;1046:97-104.
15. Zafarullah M, Li WQ, Sylvester J, Ahmad M. Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 2003;60:6-20.
16. Hall EJ. The physics and chemistry of radiation absorption. In: Hall EJ, editor. Radiobiology for the radiologist, 4th edn. Philadelphia: JB Lippincott; 1994. p. 1-13.
17. Shinomiya N. New concepts in radiation-induced apoptosis: 'premitotic apoptosis' and 'postmitotic apoptosis'. J Cell Mol Med 2001;5:240-53.
18. Withers HR. Biologic basis of radiation therapy. In: Peretz CA, Brady LW, editors. Principles and practice of radiation oncology, 2nd edn. Philadelphia: JB Lippincott; 1992. p. 64-96.
19. Rybak LP, Whitworth CA. Ototoxicity: therapeutic opportunities. Drug Discov Today 2005;10:1313-21.
20. Sha SH, Taylor R, Forge A, Schacht J. Differential vulnerability of basal and apical hair cells is based on intrinsic susceptibility to free radicals. Hear Res 2001;155:1-8.