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Carboxylated silica beads (Formula presented) diameter, from Bangs labs (Formula presented) (Formula presented) solids) were gently mixed with tris (hydroxymethyl) aminomethane solution (50 mg/0.5 ml) for 2 h at room temperature. The beads were washed three times with 0.5 ml distilled water and suspended in 1 ml microtubule polymerization buffer
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