A sensitive multiplex assay for piRNA expression

Biochemical and Biophysical Research Communications

Volumn 369, Issue 4, 2008, Pages 1190-1194

  Tang, Fuchou ^a   Hayashi, Katsuhiko ^a   Kaneda, Masahiro ^a   Lao, Kaiqin ^b   Surani, M Azim ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

Barcode; Mili; Miwi; piRNA; Real time PCR

Indexed keywords

ARGONAUTE PROTEIN; PIWI PROTEIN; PROTEIN MILI; PROTEIN MIWI; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MATURATION; GERM CELL; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIMORDIAL GERM CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA PROCESSING; RNA SYNTHESIS; SPERMATOGENESIS;

ANIMALS; BLOTTING, NORTHERN; FEMALE; GERM CELLS; MALE; MICE; OVARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; SENSITIVITY AND SPECIFICITY; TESTIS;

EID: 41249103131     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.03.035     Document Type: Article

References (15)

1. Carthew R.W. Molecular biology. A new RNA dimension to genome control. Science 313 (2006) 305-306
2. Lin H. piRNAs in the germ line. Science 316 (2007) 397
3. Hartig J.V., Tomari Y., and Forstemann K. piRNAs-the ancient hunters of genome invaders. Genes Dev. 21 (2007) 1707-1713
4. Lau N.C., Seto A.G., Kim J., Kuramochi-Miyagawa S., Nakano T., Bartel D.P., and Kingston R.E. Characterization of the piRNA complex from rat testes. Science 313 (2006) 363-367
5. Aravin A., Gaidatzis D., Pfeffer S., Lagos-Quintana M., Landgraf P., Iovino N., Morris P., Brownstein M.J., Kuramochi-Miyagawa S., Nakano T., et al. A novel class of small RNAs bind to MILI protein in mouse testes. Nature 442 (2006) 203-207
6. Girard A., Sachidanandam R., Hannon G.J., and Carmell M.A. A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature 442 (2006) 199-202
7. Grivna S.T., Beyret E., Wang Z., and Lin H. A novel class of small RNAs in mouse spermatogenic cells. Genes Dev. 20 (2006) 1709-1714
8. Ro S., Park C., Song R., Nguyen D., Jin J., Sanders K.M., McCarrey J.R., and Yan W. Cloning and expression profiling of testis-expressed piRNA-like RNAs. RNA 13 (2007) 1693-1702
9. Kuramochi-Miyagawa S., Kimura T., Ijiri T.W., Isobe T., Asada N., Fujita Y., Ikawa M., Iwai N., Okabe M., Deng W., et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131 (2004) 839-849
10. Carmell M.A., Girard A., van de Kant H.J., Bourc'his D., Bestor T.H., de Rooij D.G., and Hannon G.J. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev. Cell. 12 (2007) 503-514
11. Deng W., and Lin H. miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev. Cell. 2 (2002) 819-830
12. Grivna S.T., Pyhtila B., and Lin H. MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis. Proc. Natl. Acad. Sci. USA 103 (2006) 13415-13420
13. Aravin A.A., Sachidanandam R., Girard A., Fejes-Toth K., and Hannon G.J. Developmentally regulated piRNA clusters implicate MILI in transposon control. Science 316 (2007) 744-747
14. Chen C., Ridzon D.A., Broomer A.J., Zhou Z., Lee D.H., Nguyen J.T., Barbisin M., Xu N.L., Mahuvakar V.R., Andersen M.R., Lao K.Q., Livak K.J., and Guegler K.J. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33 (2005) e179
15. Tang F., Hajkova P., Barton S.C., Lao K., and Surani M.A. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res. 34 (2006) e9