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Volumn 77, Issue 3, 2008, Pages

Model for hand-over-hand motion of molecular motors

Author keywords

[No Author keywords available]

Indexed keywords

MATHEMATICAL MODELS; MOLECULAR STRUCTURE; PARAMETER ESTIMATION;

EID: 41149162149     PISSN: 15393755     EISSN: 15502376     Source Type: Journal    
DOI: 10.1103/PhysRevE.77.031915     Document Type: Article
Times cited : (14)

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    • In our model we assume that, in the rest state, the two heads are bounded to the microtubule, a situation which would correspond to a large ATP concentration. In any case, this is still an open question, see BIOJAU 0006-3495 10.1529/biophysj.106.100677
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    • Note that at the moment we are using the same constant l0 for the natural length of the neck, Eq. 4, and the period of the microtubule lattice, Eq. 6. This constraint will be relaxed later in Sec. 3.
    • Note that at the moment we are using the same constant l0 for the natural length of the neck, Eq. 4, and the period of the microtubule lattice, Eq. 6. This constraint will be relaxed later in Sec. 3.
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    • As the ATP gives to the head the energy needed to jump over the potential barrier, the amplitude V0 of the X potential can be taken to be the energy extracted from a single ATP molecule, EATP (looking at Ref., at room temperature, V0 EATP 20 kB T=0.5 eV, therefore, T 0.05). As the external load Q0 needed to make the system go backward is known, it can be also used to fix V0, as this force is used in making the particle climb over the smoothest slope of the potential considered. Therefore, using xM =0.5, which makes the slope considered be 1.5 l0 meters width, V0 = Q0 (1.5 l0) =0.54 eV, and T =0.046. The external load needed to stall the system and make it go backward has been experimentally measured in, obtaining Q0 =-7 pN.
    • As the ATP gives to the head the energy needed to jump over the potential barrier, the amplitude V0 of the X potential can be taken to be the energy extracted from a single ATP molecule, EATP (looking at Ref., at room temperature, V0 EATP 20 kB T=0.5 eV, therefore, T 0.05). As the external load Q0 needed to make the system go backward is known, it can be also used to fix V0, as this force is used in making the particle climb over the smoothest slope of the potential considered. Therefore, using xM =0.5, which makes the slope considered be 1.5 l0 meters width, V0 = Q0 (1.5 l0) =0.54 eV, and T =0.046. The external load needed to stall the system and make it go backward has been experimentally measured in, obtaining Q0 =-7 pN.


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