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The point on if one or two heads are bounded to the microtubule in the rest state is controversial. A recent paper [NATUAS 0028-0836 10.1038/nature06346
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Note that at the moment we are using the same constant l0 for the natural length of the neck, Eq. 4, and the period of the microtubule lattice, Eq. 6. This constraint will be relaxed later in Sec. 3.
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As the ATP gives to the head the energy needed to jump over the potential barrier, the amplitude V0 of the X potential can be taken to be the energy extracted from a single ATP molecule, EATP (looking at Ref., at room temperature, V0 EATP 20 kB T=0.5 eV, therefore, T 0.05). As the external load Q0 needed to make the system go backward is known, it can be also used to fix V0, as this force is used in making the particle climb over the smoothest slope of the potential considered. Therefore, using xM =0.5, which makes the slope considered be 1.5 l0 meters width, V0 = Q0 (1.5 l0) =0.54 eV, and T =0.046. The external load needed to stall the system and make it go backward has been experimentally measured in, obtaining Q0 =-7 pN.
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As the ATP gives to the head the energy needed to jump over the potential barrier, the amplitude V0 of the X potential can be taken to be the energy extracted from a single ATP molecule, EATP (looking at Ref., at room temperature, V0 EATP 20 kB T=0.5 eV, therefore, T 0.05). As the external load Q0 needed to make the system go backward is known, it can be also used to fix V0, as this force is used in making the particle climb over the smoothest slope of the potential considered. Therefore, using xM =0.5, which makes the slope considered be 1.5 l0 meters width, V0 = Q0 (1.5 l0) =0.54 eV, and T =0.046. The external load needed to stall the system and make it go backward has been experimentally measured in, obtaining Q0 =-7 pN.
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