Identification of a new isoform of the human estrogen receptor-alpha (hER-α) that is encoded by distinct transcripts and that is abide to repress hER-α activation function 1

EMBO Journal

Volumn 19, Issue 17, 2000, Pages 4688-4700

  Flouriot, Gilles ^a,b   Brand, Heike ^a   Denger, Stefanie ^a   Metivier, Raphaël ^b   Kos, Martin ^a   Reid, George ^a   Sonntag Buck, Vera ^a   Gannon, Frank ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UPRES A CNRS 6026   (France)

Author keywords

Activation functions; Estrogen receptor; Gene regulation; Isoforms; MCF7

Indexed keywords

ESTROGEN RECEPTOR ALPHA; TRANSCRIPTION FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING COMPETITION; BINDING SITE; CONTROLLED STUDY; DIMERIZATION; DNA BINDING; EXON; GENE ACTIVATION; GENE FUNCTION; GENE REPRESSION; GENETIC ANALYSIS; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROMOTER REGION; TRANSACTIVATION;

BASE SEQUENCE; BREAST NEOPLASMS; CELL DIVISION; DIMERIZATION; DNA; ESTROGEN RECEPTOR ALPHA; HUMANS; PROTEIN ISOFORMS; RECEPTORS, ESTROGEN; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0034283016     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (62)

1. Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor
2. The oestrogen receptor
3. A mitochondrial RNA maturase gene transferred to the yeast nucleus can control mitochondrial mRNA splicing
4. Alternative initiation of translation accounts for a 67/45 kDa dimorphism of the human estrogen receptor ERα
5. Gene regulation by steroid hormones
6. Role of the two activating domains of the oestrogen receptor in the cell-type and promoter-context dependent agonistic activity of the anti-oestrogen 4-hydroxytamoxifen
7. Luciferase reporter assay in mammalian cells
8. Dissociation between steroid receptor expression and cell proliferation in the human breast
9. Estrogen receptor null mice: What have we learned and where will they lead us?
10. Analysis of transcription and estrogen insensitivity in the female mouse after targeted disruption of the estrogen receptor gene
11. Control of human breast cancer by estrogen growth factors and oncogenes
12. Cell cycle phase dependence of estrogen and epidermal growth factor (EGF) receptor expression in MCF-7 cells: Implications in antiestrogen and EGF cell responsiveness
13. The steroid thyroid hormone receptor superfamily
14. An S1 nuclease mapping method for detection of low abundance transcripts
15. Differentially expressed messenger RNA isoforms of the human estrogen receptor-α gene are generated by alternative splicing and promoter usage
16. Activation of the ovalbumin gene by the estrogen receptor involves the fos-jun complex
17. Sex determination and sex differentiation
18. A new technique for the assay of infectivity of human adenovims 5 DNA
19. Human estrogen receptor cDNA: Sequence expression and homology to v-erbA
20. A versatile in vivo and in vitro eukaryotic expression vector for protein engineering
21. Immunochemical studies of estrogen receptors
22. Identification of novel chicken estrogen receptor-α messenger ribonucleic acid isoforms generated by alternative splicing and promoter usage
23. Two functionally different protein isoforms are produced from the chicken estrogen receptor-α gene
24. Heme regulates transcription of the CYCI gene of S.cerevisiae via an upstream activation site
25. Estrogens as a cause of human cancer: The Richard and Hinda Rosenthal Foundation award lecture
26. Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-β
27. Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells
28. Murine isoforms of retinoic acid receptor γ with specific patterns of expression
29. Tissue-specific expression of multiple mRNA variants of the mouse estrogen receptor α gene
30. The scanning model for translation: An update
31. Cloning of a novel estrogen receptor expressed in rat prostate and ovary
32. Multiple isoforms of the mouse retinoic acid receptor α are generated by alternative splicing and differential induction by retinoic acid
33. Transfection of human estrogen receptor (ER) cDNA into ER-negative mammalian cell lines
34. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene
35. Prognostic factors in primary breast cancer
36. Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast
37. Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells
38. pEF-BOS, a powerful mammalian expression vector
39. ERβ: Identification and characterization of a novel human receptor
40. Estrogens and progestins
41. Identification of a third autonomous activation domain within the human estrogen receptor
42. Differential ligand activation of estrogen receptors ERα and ERβ at API sites
43. Dominant-negative regulation of trans-activation by the rat androgen receptor: Roles of the N-terminal domain and heterodimer formation
44. Synergism between a half-site and an imperfect estrogen-responsive element and cooperation with COUP-TFI are required for estrogen receptor (ER) to achieve a maximal estrogen-stimulation of rainbow trout ER gene
45. Ligand-dependent and -independent function of the transactivation regions of the human estrogen receptor in yeast
46. Regulation of estrogen receptor messenger ribonucleic acid and protein levels in human breast cancer cell lines by sex steroid hormones, their antagonists and growth factors
47. Pattern of distribution of cells positive for estrogen receptor α and progesterone receptor in relation to proliferating cells in the mammary gland
48. Regulation of the estrogen receptor in MCF-7 cells by estradiol
49. Genomic organization and alternative promoter usage of two thyroid hormone receptor genes in Xenopus laevis
50. Responsiveness of the 5'-flanking region of the brain type isozyme of creatine kinase to estrogens and antiestrogen
51. Multiple hormone interactions in the developmental biology of the mammary gland
52. Dominant activity of activation function 1 (AF-1) and differential stoichiometric requirements for AF-1 and -2 in the estrogen receptor α-β heterodimeric complex
53. Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions
54. The nucleotide sequence of the chicken apo very low density lipoprotein II gene
55. Anti-estrogens inhibit the mitogenic effect of growth factors on breast cancer cells in the total absence of estrogens
56. Structure-function analysis of the hormone-binding domain of the human estrogen receptor by region-specific mutagenesis and phenotypic screening in yeast
57. An N-terminal deletion mutant of estrogen receptor exhibits increased synergism with upstream activators and enhanced binding to the estrogen response element
58. Differentially expressed isoforms of mouse retinoic acid receptor β are generated by usage of two promoters and alternative splicing