Preparation of Na,K-ATPase specifically modified on the anti-fluorescein antibody-inaccessible site by fluorescein 5'-isothiocyanate

Analytical Biochemistry

Volumn 287, Issue 2, 2000, Pages 303-312

  Lin, Shwu Hwa ^a   Faller, Larry D ^b  

^a University of Texas Medical Branch School of Medicine   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Conformational change; Energy transfer; FITC; Na,K ATPase

Indexed keywords

AMINO ACIDS; ANTIBODIES; ENZYMES; FLUORESCENCE; RATE CONSTANTS; REAGENTS;

ANTI-FLUORESCEIN; CONFORMATIONAL CHANGE; CYSTEINE RESIDUES; ENERGY TRANSFER MEASUREMENT; EQUILIBRIUM TITRATIONS; FITC; KINETIC CONSTANT; MODIFIED ENZYMES;

ENERGY TRANSFER;

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ANTIBODY; CYSTEINE; DITHIOTHREITOL; FLUORESCEIN; FLUORESCEIN ISOTHIOCYANATE; SODIUM ION; THIOL REAGENT;

ANALYTIC METHOD; ARTICLE; CONFORMATIONAL TRANSITION; ENERGY TRANSFER; ENZYME MODIFICATION; ENZYME SPECIFICITY; FLUORESCENCE; KINETICS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TITRIMETRY;

EID: 0034672118     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4828     Document Type: Article

References (42)

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6. +-ATPases
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14. +-ATPase
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16. +-ATPase
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19. +-ATPase
20. Protein measurement with the Folin-phenol reagent
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28. +)-activated ATPase. XLVI. Effect of cation-induced conformational changes on sulfhydryl group modification
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37. +-ATPase: Their lateral motions and proximity to the cardiac glycoside site
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42. Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 and Å resolution