One-step affinity tag purification of full-length recombinant human AP-1 complexes from bacterial inclusion bodies using a polycistronic expression system

Protein Expression and Purification

Volumn 59, Issue 1, 2008, Pages 144-152

  Wang, Wei Ming ^a,c,d   Lee, A Young ^a   Chiang, Cheng Ming ^a,b,c  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d TRI SERVICE GENERAL HOSPITAL   (Taiwan)

Author keywords

Affinity purification; AP 1; FLAG tag; Hexahistidine tag; Inclusion bodies

Indexed keywords

RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR AP 1;

ARTICLE; BIOSYNTHESIS; CELL INCLUSION; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLASMID; ULTRASTRUCTURE;

CLONING, MOLECULAR; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; PLASMIDS; RECOMBINANT PROTEINS; TRANSCRIPTION FACTOR AP-1;

BACTERIA (MICROORGANISMS); HUMAN PAPILLOMAVIRUS;

EID: 40949111823     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.01.016     Document Type: Article

References (28)

1. Eckert R.L., Crish J.F., Banks E.B., and Welter J.F. The epidermis: genes on-genes off. J. Invest. Dermatol. 109 (1997) 501-509
2. Shaulian E., and Karin M. AP-1 as a regulator of cell life and death. Nat. Cell Biol. 4 (2002) E131-E136
3. Eferl R., and Wagner E.F. AP-1: a double-edged sword in tumorigenesis. Nat. Rev. Cancer 3 (2003) 859-868
4. Gentz R., Rauscher F.J., Abate C., and Curran T. Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains. Science 243 (1989) 1695-1699
5. Turner R., and Tjian R. Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers. Science 243 (1989) 1689-1694
6. Abate C., Luk D., Gagne E., Roeder R.G., and Curran T. Fos and jun cooperate in transcriptional regulation via heterologous activation domains. Mol. Cell. Biol. 10 (1990) 5532-5535
7. Abate C., Luk D., Gentz R., Rauscher III F.J., and Curran T. Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly. Proc. Natl. Acad. Sci. USA 87 (1990) 1032-1036
8. Abate C., Luk D., and Curran T. Transcriptional regulation by Fos and Jun in vitro: interaction among multiple activator and regulatory domains. Mol. Cell. Biol. 11 (1991) 3624-3632
9. Angel P., and Karin M. The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim. Biophys. Acta 1072 (1991) 129-157
10. Kerpolla T.K., and Curran T. Fos-Jun heterodimers and Jun homodimers bend DNA in opposite orientations: implications for transcription factor cooperativity. Cell 66 (1991) 317-326
11. Sutherland J.A., Cook A., Bannister A.J., and Kouzarides T. Conserved motifs in Fos and Jun define a new class of activation domain. Genes Dev. 6 (1992) 1810-1819
12. Karin M. The regulation of AP-1 activity by mitogen-activated protein kinases. J. Biol. Chem. 270 (1995) 16483-16486
13. Xanthoudakis S., and Curran T. Identification and characterization of Ref-1, a nuclear protein that facilitates AP-1 DNA-binding activity. EMBO J. 11 (1992) 653-665
14. Fehrmann F., and Laimins L.A. Human papillomaviruses: targeting differentiating epithelial cells for malignant transformation. Oncogene 22 (2003) 5201-5207
15. Wu S.-Y., Lee A.-Y., Hou S.Y., Kemper J.K., Erdjument-Bromage H., Tempst P., and Chiang C.-M. Brd4 links chromatin targeting to HPV transcriptional silencing. Genes Dev. 20 (2006) 2383-2396
16. Ferguson H.A., and Goodrich J.A. Expression and purification of recombinant human c-Fos/c-Jun that is highly active in DNA binding and transcriptional activation in vitro. Nucleic Acids Res. 29 (2001) E98
17. Li C., Schwabe J.W., Banayo E., and Evans R.M. Coexpression of nuclear receptor partners increases their solubility and biological activities. Proc. Natl. Acad. Sci. USA 94 (1997) 2278-2283
18. Tan S. A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli. Protein Expr. Purif. 21 (2001) 224-234
19. Harrison R.J., McNeil G.P., and Dobner P.R. Synergistic activation of neurotensin/neuromedin N gene expression by c-Jun and glucocorticoids: novel effects of Fos family proteins. Mol. Endocrinol. 9 (1995) 981-993
20. Short J.D., and Pfarr C.M. Translational regulation of the JunD messenger RNA. J. Biol. Chem. 277 (2002) 32697-32705
21. Chiang C.-M., and Roeder R.G. Expression and purification of general transcription factors by FLAG epitope-tagging and peptide elution. Pept. Res. 6 (1993) 62-64
22. Chiang C.-M., Ge H., Wang Z., Hoffmann A., and Roeder R.G. Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III. EMBO J. 12 (1993) 2749-2762
23. Zhou W., Chow L.T., and Broker T.R. Transcription activities of human papillomavirus type 11 E6 promoter-proximal elements in raft and submerged cultures of foreskin keratinocytes. J. Virol. 71 (1997) 8832-8840
24. Hou S.Y., Wu S.-Y., Zhou T., Thomas M.C., and Chiang C.-M. Alleviation of human papillomavirus E2-mediated transcriptional repression via formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex. Mol. Cell. Biol. 20 (2000) 113-125
25. Hou S.Y., Wu S.-Y., and Chiang C.-M. Transcriptional activity among high and low risk human papillomavirus E2 proteins correlates with E2 DNA binding. J. Biol. Chem. 277 (2002) 45619-45629
26. Thomas M.C., and Chiang C.-M. E6 oncoprotein represses p53-dependent gene activation via inhibition of protein acetylation independently of inducing p53 degradation. Mol. Cell 17 (2005) 251-264
27. Tan S., Kern R.C., and Selleck W. The pST44 polycistronic expression system for producing protein complexes in Escherichia coli. Protein Expr. Purif. 40 (2005) 385-395
28. Singh S.M., and Panda A.K. Solubilization and refolding of bacterial inclusion body proteins. J. Biosci. Bioeng. 99 (2005) 303-310