Improving CMC-derivatization of pseudouridine in RNA for mass spectrometric detection

Analytica Chimica Acta

Volumn 612, Issue 2, 2008, Pages 173-181

  Durairaj, Anita ^a   Limbach, Patrick A ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

Author keywords

2 Methylthio N6 isopentenyladenosine; Endonucleases; Matrix assisted laser desorption ionization mass spectrometry; N cyclohexyl N (4 methylmorpholinium)ethylcarbodiimide p tosylate derivatization; Pseudouridine; Ribonucleic acid signature products; RNase T1; Transfer ribonucleic acid

Indexed keywords

ENDONUCLEASES; MATRIX-ASSISTED LASER DESORPTION; PSEUDOURIDINE; RIBONUCLEIC ACID SIGNATURE PRODUCTS;

BACTERIA; ESCHERICHIA COLI; OPTIMIZATION; RNA; TRANSCRIPTION;

NUCLEOTIDES;

6 N (3,3 DIMETHYLALLYL)ADENOSINE; 6 N ISOPENTENYL 2 METHYLTHIOADENOSINE; BACTERIAL RNA; IMIDE; MORPHOLINE DERIVATIVE; N CYCLOHEXYL N' BETA (4 METHYLMORPHOLINIUM)ETHYLCARBODIIMIDE; NUCLEOSIDE; PSEUDOURIDINE; RIBONUCLEASE; RNA; TYROSINE TRANSFER RNA; UNCLASSIFIED DRUG; URIDINE;

ANALYTIC METHOD; ARTICLE; DERIVATIZATION; ESCHERICHIA COLI; MASS; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; PRIORITY JOURNAL;

ESCHERICHIA COLI; EXONUCLEASES; IMIDES; MORPHOLINES; OLIGONUCLEOTIDES; PSEUDOURIDINE; RIBONUCLEASE T1; RNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 40849139057     PISSN: 00032670     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aca.2008.02.026     Document Type: Article

References (25)

1. Ho N.W.Y., and Gilham P.T. Biochemistry 10 (1971) 3651
2. Naylor R., Ho N.W.Y., and Gilham P.T. J. Am. Chem. Soc. 87 (1965) 4209
3. Wani A.A., Sullivan J.K., and Lebowitz J. Nucleic Acids Res. 17 (1989) 9957
4. Lebowitz J., Chaudhuri A.K., Gonenne A., and Kitos G. Nucleic Acids Res. 4 (1977) 1695
5. Flashner M.S., Katopes M.A., and Lebowitz J. Nucleic Acids Res. 4 (1977) 1713
6. Bakin A., and Ofengand J. Biochemistry 32 (1993) 9754
7. Bakin A., Lane B.G., and Ofengand J. Biochemistry 33 (1994) 13475
8. Hansen M.A., Kirpekar F., Ritterbushch W., and Vester B. RNA 3 (2002) 202
9. Patteson K.G., Rodicio L.P., and Limbach P.A. Nucleic Acids Res. 29 (2001) e49
10. Campo D.L., Recinos C., Yanez G., Pomerantz S.C., Guymon R., Crain P.F., McCloskey J.A., and Ofengand J. RNA 11 (2005) 210
11. Ofengand J., Campo D.M., and Kaya Y. Methods 25 (2001) 365
12. Mengel-Jorgensen J., and Kirpekar F. Nucleic Acids Res. 30 (2002) e135
13. Emmerechts G., Herdewijn P., and Rozenski J. J. Chromatogr. B 825 (2005) 233
14. Pomerantz S.C., and McCloskey J.A. Anal. Chem. 77 (2005) 4687
15. Buchhaupt M., Peifer C., and Entian K. Anal. Biochem. 361 (2007) 102
16. Hale P., Woodward R.S., and Lebowitz J. J. Biol. Chem. 258 (1983) 7828
17. Novack D.F., Casna N.J., Fischer S.G., and Ford J.P. Proc. Natl. Acad. Sci. U.S.A. 83 (1986) 586
18. Wani A.A., and Yamasaki E.F. Biochim. Biophys. Acta 259 (1991) 1088
19. Kirpekar F., Douthwaite S., and Roepstorff P. RNA 6 (2000) 296
20. Limbach P.A. Mass Spectrom. Rev. 15 (1996) 297
21. Limbach P.A., Crain P.F., and McCloskey J.A. Nucleic Acids Res. 22 (1994) 2183
22. Meng Z., and Limbach P.A. Anal. Chem. 77 (2005) 1891
23. Sprinzl M., and Vassilenko K.S. Nucleic Acids Res. 33 (2005) D139
24. Hossain M., and Limbach P.A. RNA 13 (2007) 295
25. Driscoll M.D., Sathya G., Saidi L.F., DeMott M.S., Hilf R., and Bambara R.F. Mol. Endocrinol. 13 (1999) 958