FIT probes: Peptide nucleic acid probes with a fluorescent base surrogate enable real-time DNA quantification and single nucleotide polymorphism discovery

Analytical Biochemistry

Volumn 375, Issue 2, 2008, Pages 318-330

  Socher, Elke ^a   Jarikote, Dilip V ^a   Knoll, Andrea ^a   Röglin, Lars ^a   Burmeister, Jens ^b   Seitz, Oliver ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b BAYER AG   (Germany)

Author keywords

Fluorescent probes; Homogeneous DNA detection; Hybridization; Intercalation; PNA; qPCR; Real time PCR; Single base mutation; SNP; Thiazole orange

Indexed keywords

AMINO ACIDS; CITRUS FRUITS; DNA; EXCITED STATES; FLUORESCENCE; INTERCALATION; NUCLEOTIDES; PEPTIDES; POLYMORPHISM; PROBES;

DNA DETECTION; FLUORESCENT PROBES; HYBRIDIZATION; QPCR; REAL-TIME PCR; SINGLE BASE MUTATION; THIAZOLE ORANGES;

POLYMERASE CHAIN REACTION;

AMINOETHYLGLYCINE; GENOMIC DNA; GLYCINE DERIVATIVE; ORNITHINE; PEPTIDE NUCLEIC ACID; THIAZOLE ORANGE; UNCLASSIFIED DRUG;

ALLELISM; ARTICLE; BASE MISPAIRING; CFTR GENE; DNA DETERMINATION; DNA HYBRIDIZATION; FLUORESCENCE; GENE; HUMAN; MUTATION; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION; SINGLE NUCLEOTIDE POLYMORPHISM;

EID: 40649120420     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.01.009     Document Type: Article

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