Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside

Journal of the American Chemical Society

Volumn 125, Issue 17, 2003, Pages 4972-4973

  Okamoto, Akimitsu ^a   Tainaka, Kazuki ^a   Saito, Isao ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACID; NUCLEOSIDE; OLIGODEOXYNUCLEOTIDE; PURINE;

ARTICLE; BASE PAIRING; FLUORESCENCE; GENE TARGETING; HYBRIDIZATION; NUCLEAR MAGNETIC RESONANCE; NUCLEIC ACID STRUCTURE; SINGLE NUCLEOTIDE POLYMORPHISM; SPECTROFLUOROMETRY;

EID: 0037473574     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja034090u     Document Type: Article

References (20)

1. (a) Wojczewski, C.; Stolze, K.; Engels, J. W. Synlett 1999, 1667-1678.
2. (b) Hawkins, M. E. Cell Biochem. Biophys. 2001, 34, 257-281.
3. (a) Ward, D. C.; Reich, E.; Stryer, L. J. Biol. Chem. 1969, 244, 1228-1237.
4. (b) Menger, M.; Tuschl, T.; Eckstein, F.; Porschke, D. Biochemistry 1996, 35, 14710-14716.
5. (c) Lacourciere, K. A.; Stivers, J. T.; Marino, J. P. Biochemistry 2000, 39, 5630-5641.
6. (a) Secrist, J. A., III; Barrio, J. R.; Leonard, N. J. Science 1972, 175, 646-647.
7. (b) Holmén, A.; Albinsson, B.; Nordén, B. J. Phys. Chem. 1994, 98, 13460-13469 and references therein.
8. (a) Seela, F.; Zulauf, M.; Sauer, M.; Deimel, M. Helv. Chim. Acta 2000, 83, 910-927.
9. (b) Hurley, D. J.; Seaman, S. E.; Mazura, J. C.; Tor, Y. Org. Lett. 2002, 4, 2305-2308.
10. (a) Strässler, C.; Davis, N. E.; Kool, E. T. Helv. Chito Chim. Acta 1999, 82, 2160-2171.
11. (b) Kool, E. T. Acc. Chem. Res. 2002, 35, 936-943.
12. The fluorescence quantum yields (Φ) of ODN(BPP)/ODN and ODN-(BPP)/ODN were 0.026 and 0.025, respectively.
13. m's of the duplexes (2,5 μM) were measured in 50 mM sodium phosphate and 100 mM sodium chloride, pH = 7.0. The absorbance of the duplexes was monitored at 260 nm from 10 to 80 °C using a heating rate of 1 °C/min.
14. (a) Kupferschmitt, G.; Schmidt, J.; Schmidt, T.; Fera, B.; Buck, F.; Ruterjaus, H. Nucleic Acids Res. 1987. 15, 6225-6241.
15. (b) Ariza, X.; Bou, V.; Vilarrasa, J. J. Am. Chem. Soc. 1995, 117, 3665-3673.
16. Yamauchi, K.; Tanabe, T.; Kinoshita, M. J. Org. Chem. 1979, 44, 638-639.
17. 1-methylguanine is very loose.
18. (a) Iwasaki, H.; Ota, N.; Nakajima, T.; Shinohara, Y.; Kodaira, M.; Kajita, M.; Emi, M. J. Hum. Genet. 2001, 46, 32-34.
19. (b) http://snp.ims.u-tokyo.ac.jp/cgi-bin/SnpInfo.cgi?SNP_ID=IMS-JST037908.
20. When the flanking base pair is a G/C base pair, the fluorescence of BPP is considerably suppressed. Thus, the SNP typing method would be inaccurate for the sequence containing a G/C base pair flanking to SNP site.