A high sensitivity amperometric biosensor using a monomolecular layer of laccase as biorecognition element

Biosensors and Bioelectronics

Volumn 20, Issue 2, 2004, Pages 315-321

  Vianello, Fabio ^a   Cambria, Antonio ^b   Ragusa, Santa ^b   Cambria, Maria Teresa ^b   Zennaro, Lucio ^a   Rigo, Adelio ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b UNIVERSITY OF CATANIA   (Italy)

Author keywords

Amperometric biosensor; Enzyme monolayer; Laccase; Olive oil mill wastewater; Phenol determination; Pollution monitoring

Indexed keywords

CARBON; ELECTROCHEMICAL ELECTRODES; ENZYME IMMOBILIZATION; ENZYME KINETICS; PHENOLS; SELF ASSEMBLY; WASTEWATER;

LACCASES; OLIVE OIL MILLS;

ENZYME SENSORS;

3 MERCAPTOPROPIONIC ACID; CARBON; CYANAMIDE; ELEMENT; GLASS; GOLD; HYDROQUINONE; LACCASE; OLIVE OIL; PHENOL; SODIUM BOROHYDRIDE;

ACCURACY; AMPEROMETRIC BIOSENSOR; AQUEOUS SOLUTION; ARTICLE; CHEMISTRY; CONCENTRATION (PARAMETERS); COVALENT BOND; DEVICE; ELECTRODE; ENZYME SUBSTRATE; FLOW; HALF LIFE TIME; INCUBATION TIME; MICROORGANISM; MOLECULAR RECOGNITION; MOLECULE; OXIDATION; RIGIDOPORUS LIGNOSUS; SIGNAL TRANSDUCTION; SURFACE PROPERTY; WASTE WATER;

BIOSENSING TECHNIQUES; COATED MATERIALS, BIOCOMPATIBLE; ELECTROCHEMISTRY; ELECTRODES; ENVIRONMENTAL MONITORING; ENZYMES, IMMOBILIZED; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; INDUSTRIAL WASTE; LACCASE; PHENOLS; PLANT OILS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; WATER POLLUTANTS, CHEMICAL;

RIGIDOPORUS MICROPORUS;

EID: 4043151312     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2004.01.022     Document Type: Article

References (25)

1. Angerosa F., D'Alessandro N., Konstantinou P., Di Giacinto L. GC-MS evaluation of phenolic compounds in virgin olive oil. J. Agric. Food Chem. 42:1995;1802-1807
2. Bartak P., Frnkova P., Cap L. Determination of phenols using simultaneous steam distillation-extraction. J. Chromatogr. A. 867:2000;281-287
3. Bertrand T., Jolivalt C., Caminade E., Joly N., Mougin C., Briozzo P. Purification and preliminary crystallographic study of Trametes versicolor laccase in its native form. Acta Crystallogr. D. 58:2002;319-321
4. Bosch F., Font G., Manes J. Ultraviolet spectrophotometric determination of phenols in natural and waste water with iodine monobromide. Analyst. 112:1987;1335
5. Bradford M.M. A rapid and sensitive method for the quantitation of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
6. Cabrera F., Lopez R., Martinez-Bordiu A., Dupuy de Lome E., Murillo J.M. Land treatment of olive oil mill wastewater. Int. Biodeterior. Biodegrad. 38:1996;135-144
7. Calvente J.J., Kovakova Z., Sanchez M.D., Andreu R., Fawcett W.R. Desorbtion of spontaneously adsorbed and electrochemically readsorbed 2-mercaptoethanesulfonate on Au(1 1 1). Langmuir. 12:1996;5696-5703
8. Cambria M.T., Cambria A., Ragusa S., Rizzarelli S. Production, purification and properties of an extracellular laccase from Rigidoporus lignosus. Prot. Expr. Purif. 18:2000;141-147
9. D'Annibale A., Stazi S.R., Vinciguerra V., Giovannozzi-Sermanni G. Oxirane-immobilized Lentinula edodes laccase: stability and phenolic removal efficiency in olive mill wastewater. J. Biotechnol. 77:2000;265-273
10. Davies, G.J., Ducros, V., 2001. Laccases. In: Messerschmidt, A., Huber, R., Poulos, T., Wieghardt, K. (Eds.), Handbook of Metalloproteins, vol. II. Wiley, Chichester, UK, pp. 1359-1368.
11. Ducros V., Brzozowski A.M., Wilson K.S., Brown S.H., Ostergaard P., Schneider P., Yaver D.S., Pedersen A.H., Davies G.J. Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 Å resolution. Nat. Struct. Biol. 5:1998;310-316
12. Freire R.S., Duran N., Kubota L.T. Development of a laccase-based flow injection electrochemical biosensor for the determination of phenolic compounds and its application for monitoring remediation of Kraft E1 paper mill effluent. Anal. Chim. Acta. 463:2002;229-238
13. Harkin J.M., Obst J.R. Syringaldazine, an effective reagent for detecting laccase and peroxidase in fungi. Experientia. 29:1973;381-387
14. Kulys J., Vidziunaite R. Amperometric biosensor based on recombinant laccases for phenols determination. Biosens. Bioelectron. 18:2003;319-325
15. Mosca L., De Marco C., Visioli F., Cannella C. Enzymatic assay for the determination of olive oil polyphenol content: assay conditions and validation of the method. J. Agric. Food Chem. 48:2000;297-301
16. Ong C.P., Lee H.K., Li S.F. Optimization of mobile phase composition for high-performance liquid chromatographic analysis of eleven priority substituted phenols. J. Chromatogr. 464:1989;405-410
17. Rodriguez J.F., Soriaga M.P. Reductive desorption of iodine chemisorbed on smooth polycrystalline gold electrodes. J. Electrochem. Soc. 135:1988;616-618
18. Tasioula-Margari M., Okogeri O. Isolation and characterization of virgin olive oil phenolic compounds by HPLC/UV and GC-MS. J. Food Sci. 66:2001;530-534
19. Walczak M.M., Alves C.A., Lamp B.D., Porter M.D. Electrochemical and X-ray photoelectron spectroscopic evidence for differences in the binding sites of alkanethiolate monolayers chemisorbed at gold. J. Electroanal. Chem. 396:1995;103-114
20. Widrig C.A., Chung C., Porter M.D. The electrochemical desorbtion of n-alkanethiol monolayers from polycrystalline Au and Ag electrodes. J. Electroanal. Chem. 310:1991;335-359
21. Xu F. Oxidation of phenols, anilines and benzenethiols by fungal laccases: correlation between activity and redox potentials as well as halide inhibition. Biochemistry. 35:1996;7608-7614
22. Yaropolov A.I., Kharybin A.N., Emnéus J., Marko-Varga G., Gorton L. Flow-injection analysis of phenols at a graphite electrode modified with co-immobilized laccase and tyrosinase. Anal. Chim. Acta. 308:1995;137-144
23. Zhao L., Lee H.K. Determination of phenols in water using liquid phase microextraction with back extraction combined with high-performance liquid chromatography. J. Chromatogr. A. 931:2001;95-105
24. Zhongping Y., Gonzalez-Cortes A., Jourquin G., Viré J., Kauffmann J.M., Delplancke J.L. Analytical application of self assembled monolayers on gold electrodes: critical importance of surface pre-treatment. Biosens. Bioelectron. 10:1995;789-795
25. Zor T., Selinger Z. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal. Biochem. 236:1996;302-308