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note
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Hydrogel structures were prepared by photoinitiated free radical polymerization of a pre-polymer mixture containing 2-hydroxyethyl methacrylate (hydrophilic monomer, 7.7 mmol), ethylene glycol dimethacrylate (cross-linker, 0.02 mmol), 2,2-dimethoxy-2-phenylacetophenone (photoinitiator, 3 wt %), and either DMAEMA (1.4 mmol) or VP (1.1 mmol) as an ionizable monomer. Photomasks were created in Adobe Illustrator and printed on transparency films using a high-resolution commercial printer system (Linotype Herkules Imagesetter) with a resolution of 5080 dpi. Microchannels were prepared by applying Scotch double-sided tape to a 75×501 mm glass slide (Fisher Scientific) as side walls and capping with a 22×50 mm no. 1 cover glass slide. Plastic pipet tips (United Laboratory) were cut to the desired length with a razor blade and glued to both ends of the microchannel with Loctite Quick Set epoxy to serve as the inlet and outlet for the microchannel. Polymerization times using a UV source from an Olympus Epi-Pluorescent microscope (BX-60) through a near-UV filter cube (U-MNUA, type BP 360-370) with a 360-370-nm band-pass were 120 and 270 s for hydrogel-DMAEMA and hydrogel-VP, respectively. After polymerization, residual monomers were washed away with methanol and the resulting hydrogel structures were stored in deionized water and visualized in real time using a Sony CCD-IRIS/RGB color video camera attached to the Olympus microscope.
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Because these hydrogels displayed an initial dormant stage, the graphed data only displayed points after swelling was initiated.
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