Tissue-specific and inducible Cre-mediated recombination in the gut epithelium

Genesis (United States)

Volumn 39, Issue 3, 2004, Pages 186-193

  El Marjou, Fatima ^a   Janssen, Klaus Peter ^b   Chang, Benny Hun Junn ^c   Li, Mei ^d   Hindie, Valérie ^a   Chan, Lawrence ^c   Louvard, Daniel ^a   Chambon, Pierre ^d,e   Metzger, Daniel ^d   Robine, Sylvie ^a,f  

^a INSTITUT CURIE   (France)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

^d CNRS   (France)

^e INSTITUT CLINIQUE DE LA SOURIS   (France)

^f INSTITUT CURIE   (France)

Author keywords

Conditional mutagenesis; Inducible expression; Intestinal epithelium; Mouse; Progenitor cells

Indexed keywords

CRE RECOMBINASE; TAMOXIFEN; VILLIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LINEAGE; CELL RENEWAL; CONTROLLED STUDY; ENDODERM; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE INDUCTION; GENE LOCUS; GENE TARGETING; GENETIC RECOMBINATION; INTESTINE EPITHELIUM; MOUSE; NONHUMAN; ORGANOGENESIS; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; STEM CELL; TISSUE SPECIFICITY; TRANSGENIC MOUSE;

MURINAE; MUS MUSCULUS;

EID: 4043059169     PISSN: 1526954X     EISSN: None     Source Type: Journal    
DOI: 10.1002/gene.20042     Document Type: Article

References (18)

1. Abremski K, Hoess R. 1984. Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein. J Biol Chem 259:1509-1514.
2. Chambers CA. 1994. TKO'ed: lox, stock and barrel. BioEssays 16:865-868.
3. Feil R, Brocard J, Mascrez B, LeMeur M, Metzger D, Chambon P. 1996. Ligand-activated site-specific recombination in mice. Proc Natl Acad Sci U S A 93:10887-10890.
4. Fodde R, Edelmann W, Yang K, van Leeuwen C, Carlson C, Renault B, Breukel C, Alt E, Lipkin M, Khan PM, et al. 1994. A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors. Proc Natl Acad Sci U S A 91:8969-8973.
5. Janssen K-P, Marjou FE, Pinto D, Sastre X, Rouillard D, Fouquet C, Soussi T, Louvard D, Robine S. 2002. Targeted expression of oncogenic k-ras in intestinal epithelium causes spontaneous tumorigenesis in mice. Gastroenterology 123:492-504.
6. Kinzler KW, Vogelstein B. 1996. Lessons from hereditary colorectal cancer. Cell 87:159-170.
7. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
8. Leone DP, Genoud S, Atanasoski S, Grausenburger R, Berger P, Metzger D, Macklin WB, Chambon P, Suter U. 2003. Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells. Mol Cell Neurosci 22:430-440.
9. Madison BB, Dunbar L, Qiao XTT, Braunstein K, Braunstein E, Gumucio DL. 2002. Cis elements of the villin gene control expression in restricted domains of the vertical (crypt) and horizontal (duodenum, cecum) axes of the intestine. J Biol Chem 277:33275-33283.
10. Maunoury R, Robine S, Pringault E, Huet C, Guenet JL, Gaillard JA, Louvard D. 1988. Villin expression in the visceral endoderm and in the gut anlage during early mouse embryogenesis. EMBO J 7:3321-3329.
11. Metzger D, Chambon P. 2001. Site- and time-specific gene targeting in the mouse. Methods Enzymol 24:71-80.
12. Metzger D, Indra AK, Li M, Chapellier B, Glyselink NB, Chambon P. 2003. Targeted conditional somatic mutagenesis in the mouse: temporally-controlled knockout of retinoid receptors in epidermal keratinocytes. Methods Enzymol 364:379-408.
13. Pinto D, Robine S, Jaisser F, El Marjou FE, Louvard D. 1999. Regulatory sequences of the mouse villin gene that efficiently drive transgenic expression in immature and differentiated epithelial cells of small and large intestines. J Biol Chem 274:6476-6482.
14. Robine S, Jaisser F, Louvard D. 1997. Epithelial cell growth and differentiation. IV. Controlled spatiotemporal expression of transgenes: new tools to study normal and pathological states. Am J Physiol 273:759-762.
15. Saam JR, Gordon JI. 1999. Inducible gene knockouts in the small intestinal and colonic epithelium. J Biol Chem 274:38071-38082.
16. Sauer B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods Enzymol 14:381-392.
17. Soriano P. 1999. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21:70-71.
18. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350-4354.