Cloning and purification of α-neurotoxins from king cobra (Ophiophagus hannah)

Toxicon

Volumn 44, Issue 3, 2004, Pages 295-303

  He, Ying Ying ^a,b   Lee, Wei Hui ^a   Zhang, Yun ^a  

^a KUNMING INSTITUTE OF ZOOLOGY   (China)

^b CHINA THREE GORGES UNIVERSITY   (China)

Author keywords

Neurotoxin; cDNA sequence; Ophiophagus hannah; Snake venom

Indexed keywords

NEUROTOXIN; SNAKE VENOM;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; FEMALE; LETHALITY; MALE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPHIOPHAGUS HANNAH; PHYLOGENY; PRIORITY JOURNAL; PROTEIN PURIFICATION; SNAKE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BIOLOGICAL ASSAY; CHINA; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CLUSTER ANALYSIS; COBRA; COBRA VENOMS; DNA, COMPLEMENTARY; LETHAL DOSE 50; MICE; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PHYLOGENY; SEQUENCE ANALYSIS, DNA;

OPHIOPHAGUS HANNAH; SERPENTES;

EID: 4043058652     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2004.06.003     Document Type: Article

References (35)

1. Afifiyan F., Armugam A., Gopalakrishnakone P., Tan N.H., Tan C.H., Jeyaseelan K. Four new postsynaptic neurotoxins from Naja naja sputatrix venom: cDNA cloning, protein expression, and phylogenetic analysis. Toxicon. 36:1998;1871-1871
2. Chang C.C., Lee C.Y. Isolation of neurotoxins from the venom of Bungarus multicinctus and their modes of neuromuscular blocking action. Arch. Int. Pharmacodyn. 144:1963;241-257
3. Chang C.C., Huang T.Y., Kuo K.W., Chen S.W., Huang K.F., Chiou S.H. Sequence characterization of a novel alpha-neurotoxin from the king cobra (Ophiophagus hannah) venom. Biochem. Biophys. Res. Commun. 191:1993;214-223
4. Chang L.S., Lin S.K., Huang S.B., Hsiao M. Genetic organization of α-bungarotoxin from Bungarus multicinctus (Taiwan banded krait): evidence showing that the production of α-bungarotoxin isotoxins is not derived from edited mRNAs. Nucleic Acids Res. 27:1997;3970-3975
5. Chang L., Lin S., Huang H., Hsiao M. Genetic organization of alpha-bungarotoxins from Bungarus multicinctus (Taiwan banded krait): evidence showing that the production of alpha-bungarotoxin isotoxins is not derived from edited mRNAs. Nucleic Acids Res. 27:1999;3970-3975
6. Chang L., Chang C., Lin S. Differential contribution of the conserved tyrosine residues to activity and structural stability of Ophiophagus hannah alpha-neurotoxins. J. Pept. Res. 55:2000;240-245
7. Chang L.S., Liou J.C., Lin S.R., Huang H.B. Purification and characterization of a neurotoxin from the venom of Ophiophagus hannah (King Cobra). Biochem. Biophys. Res. Commun. 294:2002;574-578
8. Du X.Y., Clemetson J.M., Navdaev A., Magnenat E.M., Wells T.N., Clemetson K.J. Ophioluxin, a convulxin-like C-type lectin from Ophiophagus hannah (King cobra) is a powerful platelet activator via glycoprotein VI. J. Biol. Chem. 277:2002;35124-35132
9. Gong N., Armugam A., Jeyaseelan K. Postsynaptic short-chain neurotoxins from Pseudonaja textilis. cDNA cloning, expression and protein characterization. Eur. J. Biochem. 265:1999;982-989
10. Gong N., Armugam A., Jeyaseelan K. Molecular cloning, characterization and evolution of the gene encoding a new group of short chain a-neurotoxins in an Australian elapid, Pseudonaja textiles. FEBS Lett. 473:2000;303-310
11. Hall Z.W. Alpha neurotoxins and their relatives: foes and friends? Neuron. 23:1999;4-5
12. Jeyaseelan K., Poh S.L., Nair R., Armugam A. Structurally conserved α-neurotoxin genes encode functionally diverse proteins in the venom of Naja sputatrix. FEBS Lett. 553:2003;333-341
13. Joubert F.J. Snake venom toxins: the amino acid sequences of two toxins from Ophiophagus hannah (King cobra) venom. Biochim. Biophys. Acta. 317:1973;85-98
14. Joubert F.J. Snake venoms. The amino-acid sequence of polypeptide DE-1 from Ophiophagus hannah (King cobra) venom. Hoppe-Seyler's Z. Physiol. Chem. 358:1977;565-574
15. Joyce S.P.Y., Gong N., Fatemah A., Ma D.H., Poh S.L., Arunmozhiarasi A., Kandiah J. Snake postsynaptic neurotoxins: gene structure, phylogeny and applications in research and therapy. Biochimie. 86:2004;137-149
16. Kini R.M. Proline brackets and identification of potential functional sites in proteins: toxins to therapeutics. Toxicon. 36:1998;1659-1670
17. Kumar S., Tamura K., Jakobsen I.B., Nei M. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17:2001;1244-1245
18. Lee W.H., Zhang Y., Wang W.Y., Xiong Y.L., Gao R. Isolation and properties of a blood coagulation factor X activator from the venom of king cobra (Ophiophagus hannah). Toxicon. 33:1995;1263-1276
19. Lin N.Q., Zhang Y.S., Mu J.F., Wang W.Y., Yang C.J., Xiong Y.L. Amino-acid sequence of the neurotoxin (CM-9) from the snake venom of Quangxi king cobra. Acta Biochim. Biophys. Sin. 16:1984;592-596
20. Lin S.R., Leu L.F., Chang L.S., Chang C.C. Amino acid sequence and chemical modification of a novel alpha-neurotoxin (Oh-5) from king cobra (Ophiophagus hannah) venom. J. Biochem. 121:1997;690-695
21. Lin S.R., Chang L.S., Chang C.C. Disulfide isomers of alpha-neurotoxins from king cobra (Ophiophagus hannah) venom. Biochem. Biophys. Res. Commun. 254:1999;104-108
22. 50 determinations of snake venoms using eight to ten experimental animals. Toxicon. 24:1986;395-401
23. Menez A. Functional architectures of animal toxins: a clue to drug design. Toxicon. 36:1998;1557-1572
24. Nirthanan S., Gwee M.C. Three-finger alpha-neurotoxins and the nicotinic acetylcholine receptor, forty years on. J. Pharmacol. Sci. 94:2004;1-17
25. Nirthanan S., Gopalakrishnakone P., Gwee M.C.E., Khoo H.E., Kini R.M. Non-conventional toxins from Elapid venoms. Toxicon. 41:2003;397-407
26. Ohno M., Menez R., Ogawa T., Danse J.M., Shimohigashi Y., Fromen C., Ducancel F., Zinn-Justin S., Le Du M.H., Boulain J.C., Tamiya T., Menez A. Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution? Prog. Nucleic Acid Res. Mol. Biol. 59:1998;307-364
27. Peng S.S., Kumar T.S., Kumar K.S.K., Chang C.C., Yu C. Solution structure of toxin b, a long neurotoxin from the venom of the King cobra (Ophiophagus Hannah). J. Biol. Chem. 272:1997;7817-7823
28. Pillet L., Tremeau O., Ducancel F., Drevet P., Zinn-Justin S., Pinkasfeld S., Boulain J.C., Menez A. Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by sitedirected mutagenesis. J. Biol. Chem. 268:1993;909-916
29. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Mannual. 1989;Cold Spring Harbor, New York
30. Servent D., Delbeke S.A., Gaillard C., Corringer P.J., Changeux J.P., Menez A. Molecular characterization of the specificity of interactions of various neurotoxins on two distinct nicotinic acetylcholine receptors. Eur. J. Pharmacol. 393:2000;197-204
31. Song J., Chung M.C.M., Xiong Y., Wang W., Pu X. Purification, sequence and pharmacological studies of a new alpha-neurotoxin from Ophiophagus hannah venom. Toxicon. 32:1994;537-538
32. Tremeau O., Lemaire C., Drevet P., Pinkasfeld S., Ducancel F., Boulain J.C., Menez A. Genetic engineering of snake toxins. The functional site of erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues. J. Biol. Chem. 270:1995;9362-9369
33. Tsetlin V. Snake venom α-neurotoxins and other 'three-finger' proteins. Eur. J. Biochem. 264:1999;281-286
34. Zha H.G., Lee W.H., Zhang Y. Cloning of cDNAs encoding C-type lectins from Elapidae snakes Bungarus fasciatus and Bungarus multicinctus. Toxicon. 39:2001;1887-1892
35. Zhang Y., Lee W.H., Xiong Y.L., Wang W.Y., Zu S.W. Characterization of OhS1, an arginine/lysine amidase from the venom of king cobra (Ophiophagus hannah). Toxicon. 32:1994;615-623