Counting membrane-embedded KCNE β-subunits in functioning K + channel complexes

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 5, 2008, Pages 1478-1482

  Morin, Trevor J ^a   Kobertz, William R ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Charybdotoxin; KCNQ1; Stoichiometry

Indexed keywords

POTASSIUM CHANNEL; POTASSIUM CHANNEL KCNE1; PROTEIN SUBUNIT; CHARYBDOTOXIN; CTX CLV; CTX-CLV; DISULFIDE; DRUG DERIVATIVE; KCNE1 PROTEIN, HUMAN; POTASSIUM CHANNEL KCNQ1; UNCLASSIFIED DRUG; VOLTAGE GATED POTASSIUM CHANNEL;

ARTICLE; CHANNEL GATING; COMPLEX FORMATION; GENE MUTATION; HOMEOSTASIS; INHIBITION KINETICS; ION TRANSPORT; MEMBRANE BINDING; MEMBRANE STRUCTURE; NERVE POTENTIAL; NONHUMAN; POTASSIUM TRANSPORT; PRIORITY JOURNAL; SKELETAL MUSCLE; STOICHIOMETRY; XENOPUS; ANIMAL; CELL MEMBRANE; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; HUMAN; METABOLISM; OOCYTE; SYNTHESIS;

ANIMALS; CELL MEMBRANE; CHARYBDOTOXIN; DISULFIDES; HUMANS; KCNQ1 POTASSIUM CHANNEL; OOCYTES; POTASSIUM CHANNELS, VOLTAGE-GATED; PROTEIN SUBUNITS; XENOPUS;

EID: 40349108463     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0710366105     Document Type: Article

References (34)

1. McCrossan ZA, Abbott GW (2004) The MinK-related peptides. Neuropharmacology 47:787-821.
2. Tyson J, et al. (2000) Mutational spectrum in the cardioauditory syndrome of Jervell and Lange-Nielsen. Hum Genet 107:499-503.
3. Splawski I, et al. (2000) Spectrum of mutations in long-QT syndrome genes KVLQT1, HERG, SCN5A, KCNE1, and KCNE2. Circulation 102:1178-1185.
4. Wang KW, Goldstein SA (1995) Subunit composition of minK potassium channels. Neuron 14:1303-1309.
5. sK channel. J Biol Chem 273:34069-34074.
6. Ks pore demonstrates two MinK subunits in each channel complex. Neuron 40:15-23.
7. Tzounopoulos T, Guy HR, Durell S, Adelman JP, Maylie J (1995) min K channels form by assembly of at least 14 subunits. Proc Natl Acad Sci USA 92:9593-9597.
8. + currents. Mol Pharmacol 54:220-230.
9. + channel. J Biol Chem 261:14607-14613.
10. MacKinnon R, Cohen SL, Kuo A, Lee A, Chait BT (1998) Structural conservation in prokaryotic and eukaryotic potassium channels. Science 280:106-109.
11. + channel: Peptide and channel residues mediating molecular recognition. Neuron 12:1377-1388.
12. Barany G, Merrifield RB (1977)Anew amino protecting group removable by reduction. Chemistry of the dithiasuccinoyl (Dts) function. J Am Chem Soc 99:7363-7365.
13. Barany G, Schroll AL, Mott AW, Halsrud DA (1983) A general strategy for elaboration of the bithiocarbonyl functionality, -(C = O)SS-: Application to the synthesis of bis-(chlorocarbonyl)disulfane and related derivatives of thiocarbonic acids. J Org Chem 48:4750-4761.
14. Martinez MA, Vega JC (1986) Synthesis of O-alkyl carbonochloridothioates. Synthesis 9:760-761.
15. Shimony E, Sun T, Kolmakova-Partensky L, Miller C (1994) Engineering a uniquely reactive thiol into a cysteine-rich peptide. Protein Eng 7:503-507.
16. + channel complexes. ACS Chem Biol 2:469-473.
17. + channels lacking the 'tetramerization' domain: implications for pore structure. Nat Struct Biol 6:1122-1125.
18. KS potassium channel. Nature 384:80-83.
19. Lu J, Kobertz WR, Deutsch C (2007) Mapping the electrostatic potential within the ribosomal exit tunnel. J Mol Biol 371:1378-1391.
20. + channels. J Gen Physiol 120:203-216.
21. Levitsky K, Boersma MD, Ciolli CJ, Belshaw PJ (2005) Exo-mechanism proximity-accelerated alkylations: investigations of linkers, electrophiles and surface mutations in engineered cyclophilin-cyclosporin systems. ChemBioChem 6:890-899.
22. + channel activation kinetics in Xenopus oocyte studies. Biophys J 90:1223-1231.
23. Ks implies an α-helical transmembrane span traversing the channel corpus. Biophys J 93:2332-2340.
24. + channel assembly. Biophys J 76:2004-2017.
25. Powers ET, Powers DL (2003) A perspective on mechanisms of protein tetramer formation Biophys J 85:3587-3599.
26. Lu J, Robinson JM, Edwards D, Deutsch C (2001) T1-T1 interactions occur in ER membranes while nascent Kv peptides are still attached to ribosomes. Biochemistry 40:10934-10946.
27. + channel assembly. EMBO Rep 4:76-81.
28. + channels for efficient trafficking and cell surface expression. J Biol Chem 281:40015-40023.
29. Krumerman A, et al. (2004) An LQT mutant minK alters KvLQT1 trafficking. AmJ Physiol 286:C1453-C1463.
30. Soreq H, Seidman S (1992) Xenopus oocyte microinjection: From gene to protein. Methods Enzymol 207:225-265.
31. Goldin AL (1992) Maintenance of Xenopus laevis and oocyte injection. Methods Enzymol 207:266-279.
32. Swanson R, Folander K (1992) In vitro synthesis of RNA for expression of ion channels in Xenopus oocytes. Methods Enzymol 207:310-319.
33. Stühmer W (1992) Electrophysiological recording from Xenopus oocytes. Methods Enzymol 207:319-339.
34. Krafte DS, Lester HA (1992) Use of stage II-III Xenopus oocytes to study voltage-dependent ion channels. Methods Enzymol 207:339-345.