Quantitative evaluation of interleukin-12 p40 gene expression in peripheral blood mononuclear cells

Immunological Investigations

Volumn 37, Issue 2, 2008, Pages 143-151

  Conte, Enrico ^a,b   Nigro, Luciano ^a   Fagone, Evelina ^a   Drago, Francesco ^a   Cacopardo, Bruno ^a  

^a UNIVERSITY OF CATANIA   (Italy)

^b Department of Medical Specialties and Internal Medicine   (Italy)

Author keywords

Gene expression; IL 12; PBMC; RT real time PCR

Indexed keywords

INTERLEUKIN 12P35; INTERLEUKIN 12P40; MESSENGER RNA;

ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HOST RESISTANCE; HUMAN; HUMAN CELL; INNATE IMMUNITY; LEISHMANIA DONOVANI; LISTERIA MONOCYTOGENES; MACROPHAGE; MONOCYTE; MYCOBACTERIUM TUBERCULOSIS; NUCLEOTIDE SEQUENCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; SUPERNATANT; T LYMPHOCYTE; TOXOPLASMA GONDII;

ADOLESCENT; ADULT; AGED; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HUMANS; INTERLEUKIN-12 SUBUNIT P40; LEUKOCYTES, MONONUCLEAR; MIDDLE AGED; PHYTOHEMAGGLUTININS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 40049094505     PISSN: 08820139     EISSN: 15324311     Source Type: Journal    
DOI: 10.1080/08820130701690824     Document Type: Article

References (16)

1. Cooper, A. M., Magram, J., Ferrante, J., Orme, I. M. (1997). Interleukin 12 (IL-12) is crucial to the development of protective immunity in mice intravenously infected with mycobacterium tuberculosis. J. Exp. Med. 186:39-45.
2. Gubler, U., Chua, A. O., Schoenhaut, D. S., Dwyer, C. M., McComas, W., Motyka, R., Nabavi, N., Wolitzky, A. G., Quinn, P. M., Familletti, P. C., et al. (1991). Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. USA 88:4143-4147.
3. Hayes, M. P., Wang, J., Norcross, M. A. (1995). Regulation of interleukin-12 expression in human monocytes: selective priming by interferon-gamma of lipopolysaccharide-inducible p35 and p40 genes. Blood 86:646-650.
4. Hiramoto, J. S., Tsung, K., Bedolli, M., Norton, J. A., Hirose, R. (2004). Antitumor immunity induced by dendritic cell-based vaccination is dependent on interferon-gamma and interleukin-12. J. Surg. Res, 116:64-69.
5. Kobayashi, M., Fitz, L., Ryan, M., Hewick, R. M., Clark, S. C., Chan, S., Loudon, R., Sherman, F., Perussia, B., Trinchieri, G. (1989). Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J. Exp. Med. 170:827-845.
6. Ma, X., Chow, J. M., Gri, G., Carra, G., Gerosa, F., Wolf, S. F., Dzialo, R., Trinchieri, G. (1996). The interleukin 12 p40 gene promoter is primed by interferon gamma in monocytic cells. J. Exp. Med. 183:147-157.
7. Magram, J., Connaughton, S. E., Warrier, R. R., Carvajal, D. M., Wu, C. Y., Ferrante, J., Stewart, C., Sarmiento, U., Faherty, D. A., Gately, M. K. (1996). IL-12-deficient mice are defective in IFN gamma production and type 1 cytokine responses. Immunity 4:471-481.
8. Mattner, F., Magram, J., Ferrante, J., Launois, P., Di Padova, K., Behin, R., Gately, M.K., Louis, J. A., Alber, G. (1996). Genetically resistant mice lacking interleukin-12 are susceptible to infection with Leishmania major and mount a polarized Th2 cell response. Eur. J. Immunol. 26:1553-1559.
9. McGrath-Morrow, S., Laube, B., Tzou, S. C., Cho, C., Cleary, J., Kimura, H., Rose, N. R., Caturegli, P. (2006). IL-12 overexpression in mice as a model for Sjogren lung disease. Am. J. Physiol. Lung Cell. Mol. Physiol. 291:L837-846.
10. McInnes, I. B., Liew, F. Y. (2005). Cytokine networks-towards new therapies for rheumatoid arthritis. Nat. Clin. Pract. Rheumatol. 1:31-39.
11. Muller, U., Kohler, G., Mossmann, H., Schaub, G. A., Alber, G., Di Santo, J. P., Brombacher, F., Holscher, C. (2001). IL-12-independent IFN-gamma production by T cells in experimental Chagas' disease is mediated by IL-18. J. Immunol. 167:3346-3353.
12. Phenekos, C., Vryonidou, A., Gritzapis, A. D., Baxevanis, C. N., Goula, M., Papamichail, M. (2004). Th1 and Th2 serum cytokine profiles characterize patients with Hashimoto's thyroiditis (Th1) and Graves' disease (Th2). Neuroimmunomodulation 11:209-213.
13. Snijders, A., Hilkens, C. M., van der Pouw Kraan, T. C., Engel, M., Aarden, L. A., Kapsenberg, M. L. (1996). Regulation of bioactive IL-12 production in lipopolysaccharide-stimulated human monocytes is determined by the expression of the p35 subunit. J. Immunol. 156:1207-1212.
14. Stern, A. S., Podlaski, F. J., Hulmes, J. D., Pan, Y. C., Quinn, P. M., Wolitzky, A. G., Familletti, P.C., Stremlo, D.L., Truitt, T., Chizzonite, R., et al. (1990). Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells. Proc. Natl. Acad. Sci. USA 87:6808-6812.
15. Tsung, K., Norton, J. A. (2006). Lessons from Coley's Toxin. Surg. Oncol. 15:25-28.
16. Yoshikawa, H., Sato, K., Edahiro, S., Furukawa, Y., Maruta, T., Iwasa, K., Watanabe, H., Takaoka, S., Suzuki, Y., Takamori, M., Yamada, M. (2006). Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. J. Neuroimmunol. 175:169-175.