Curcumin inhibits FtsZ assembly: An attractive mechanism for its antibacterial activity

Biochemical Journal

Volumn 410, Issue 1, 2008, Pages 147-155

  Rai, Dipti ^a   Singh, Jay Kumar ^a   Roy, Nilanjan ^b   Panda, Dulal ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY BOMBAY   (India)

^b NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH NIPER   (India)

Author keywords

Antibacterial activity; Curcumin; Filamentation; FtsZ polymerization; GTPase activity; Z ring

Indexed keywords

CHEMICAL STABILITY; DRUG DELIVERY; ENZYME ACTIVITY; POLYMERIZATION; SELF ASSEMBLY;

ANTIBACTERIAL ACTIVITY; CYTOKINESIS;

BACTERIA;

CURCUMIN; FTSZ PROTEIN; GUANOSINE TRIPHOSPHATASE;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS SUBTILIS; CELL PROLIFERATION; DRUG INHIBITION; ENTEROCOCCUS; ENZYME ACTIVITY; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN ASSEMBLY; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CURCUMIN; CYTOSKELETAL PROTEINS; MICROBIAL SENSITIVITY TESTS; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ENTEROCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 39749130244     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070891     Document Type: Article

References (43)

1. Dai, K. and Lutkenhaus, J. (1991) ftsZ is an essential cell division gene in Escherichia coli J. Bacteriol. 173, 3500-3506
2. Nogales, E., Downing, K. H., Amos, L. A. and Lowe, J. (1998) Tubulin and FtsZ form a distinct family of GTPases. Nat. Struct. Biol. 5, 451-458
3. Errington, J., Daniel, R. A. and Scheffers, D.-J. (2003) Cytokinesis in bacteria. Microbiol. Mol. Biol. Rev. 67, 52-65
4. Romberg, L. and Levin, P. A. (2003) Assembly dynamics of the bacterial cell division protein FtsZ: poised at the edge of stability. Annu. Rev. Microbiol. 57, 125-154
5. Margolin, W. (2005) FtsZ and the division of prokaryotic cells and organelles. Nat. Rev. Mol. Cell Biol. 6, 862-871
6. Michie, K. A. and Lowe, J. (2006) Dynamic filaments of the bacterial cytoskeleton. Annu. Rev. Biochem. 75, 467-492
7. White, E. L., Suling, W. J., Ross, L. J., Seitz, L. E. and Reynolds, R. C. (2002) 2-Alkoxycarbonylaminopyridines: inhibitors of Mycobacterium tuberculosis FtsZ. J. Antimicrob. Chemother. 50, 111-114
8. Wang, J., Galgoci, A., Kodali, S., Herath, K. B., Jayasuriya, H., Dorso, K., Vicente, F., Gonzalez, A., Cully, D., Bramhill, D. et al. (2003) Discovery of a small molecule that inhibits cell division by blocking FtsZ, a novel therapeutic target of antibiotics. J. Biol. Chem. 278, 44424-44428
9. Margalit, D. N., Romberg, L., Mets, R. B., Hebert, A. M., Mitchison, T. J., Kirschner, M. W. and RayChaudhuri, D. (2004) Targeting cell division: small-molecule inhibitors of FtsZ GTPase perturb cytokinetic ring assembly and induce bacterial lethality. Proc. Natl. Acad. Sci. U.S.A. 101, 11821-11826
10. Urgaonkar, S., La Pierre, H. S., Meir, I., Lund, H., RayChaudhuri, D. and Shaw, J. T. (2005) Synthesis of antimicrobial natural products targeting FtsZ: (+/-)-dichamanetin and (+/-)-2‴-hydroxy-5″-benzylisouvarinol-B. Org. Lett. 7, 5609-5612
11. Beuria, T. K., Santra, M. K. and Panda, D. (2005) Sanguinarine blocks cytokinesis in bacteria by inhibiting FtsZ assembly and bundling. Biochemistry 44, 16584-16593
12. Stokes, N. R., Sievers, J., Barker, S., Bennett, J. M., Brown, D. R., Collins, I., Errington, V. M., Foulger, D., Hall, M., Halsey, R. et al. (2005) Novel inhibitors of bacterial cytokinesis identified by a cell-based antibiotic screening assay. J. Biol. Chem. 280, 39709-39715
13. Jaiswal, R., Beuria, T. K., Mohan, R., Mahajan, S. K. and Panda, D. (2007) Totarol inhibits bacterial cytokinesis by perturbing the assembly dynamics of FtsZ. Biochemistry 46, 4211-4220
14. Huang, Q., Kirikae, F., Kirikae, T., Pepe, A., Amin, A., Respicio, L., Slayden, R. A., Tonge, P. J. and Ojima, I. (2006) Targeting FtsZ for antituberculosis drug discovery: noncytotoxic taxanes as novel antituberculosis agents. J. Med. Chem. 49, 463-466
15. Ruby, A. J., Kuttan, G., Babu, K. D., Rajasekharan, K. N. and Kuttan, R. (1995) Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett. 94, 79-83
16. Dorai, T. and Aggarwal, B. B. (2004) Role of chemopreventive agents in cancer therapy. Cancer Lett. 215, 129-140
17. Mishra, S., Narain, U., Mishra, R. and Misra, K. (2005) Design, development and synthesis of mixed bioconjugates of piperic acid-glycine, curcumin-glycine/alanine and curcumin-glycine-piperic acid and their antibacterial and antifungal properties. Bioorg. Med. Chem. 13, 1477-1486
18. Panchatcharam, M., Miriyala, S., Gayathri, V. S. and Suguna, L. (2005) Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol. Cell. Biochem. 290, 87-96
19. Gupta, K. K., Bharne, S. S., Rathinasamy, K., Naik, N. R. and Panda, D. (2006) Dietary antioxidant curcumin inhibits microtubule assembly through tubulin binding. FEBS J. 273, 5320-5332
20. Di Mario, F., Cavallaro, L. G., Nouvenne, A., Stefani, N., Cavestro, G. M., Iori, V., Maino, M., Comparato, G., Fanigliulo, L., Morana, E. et al. (2007) A curcumin-based 1-week triple therapy for eradication of Helicobacter pylori infection: something to learn from failure? Helicobacter 12, 238-243
21. Tonnesen, H. H., de Vries, H., Karlsen, J. and Beijersbergen van Henegouwen, G. (1987) Studies on curcumin and curcuminoids. IX: investigation of the photobiological activity of curcumin using bacterial indicator systems. J. Pharm. Sci. 76, 371-373
22. Dahl, T. A., McGowan, W. M., Strand, M. A. and Srinivasan, V. S. (1989) Photokilling of bacteria by the natural dye curcumin. Arch. Microbiol. 151, 183-185
23. Azuine, M. A., Kayal, J. J. and Bhide, S. V. (1992) Protective role of aqueous turmeric extract against mutagenicity of direct-acting carcinogens as well as benzo [alpha] pyrene-induced genotoxicity and carcinogenicity. J. Cancer Res. Clin. Oncol. 118, 447-452
24. Oda, Y. (1995) Inhibitory effect of curcumin on SOS functions induced by UV irradiation. Mutat. Res. 348, 67-73
25. Sharma, A., Gautam, S. and Jadhav, S. S. (2000) Spice extracts as dose-modifying factors in radiation inactivation of bacteria. J. Agric. Food Chem. 48, 1340-1344
26. Shishu, Singla, A. K. and Kaur, I. P. (2002) Inhibitory effect of curcumin and its natural analogues on genotoxicity of heterocyclic amines from cooked food. Indian J. Exp. Biol. 40, 1365-1372
27. Pal, A. and Pal, A. K. (2005) Radioprotection of turmeric extracts in bacterial system. Acta Biol. Hung. 56, 333-343
28. Wang, Y. J., Pan, M. H., Cheng, A. L., Lin, L. I., Ho, Y. S., Hsieh, C. Y. and Lin, J. K. (1997) Stability of curcumin in buffer solutions and characterization of its degradation products. J. Pharm. Biomed. Anal. 15, 1867-1876
29. Canamares, M. V., Garcia-Ramos, J. V. and Sanchez-Cortes, S. (2006) Degradation of curcumin dye in agueous solution and on Ag nanoparticles studied by ultraviolet-visible absorption and surface-enhanced Raman spectroscopy. Appl. Spectrosc. 60, 1386-1391
30. Oetari, S., Sudibyo, M., Commandeur, J. N., Samhoedi, R. and Vermeulen, N. P. (1996) Effects of curcumin on cytochrome P450 and glutathione S-transferase activities in rat liver. Biochem. Pharmacol. 51, 39-45
31. Santra, M. K., Dasgupta, D. and Panda, D. (2005) Deuterium oxide promotes assembly and bundling of FtsZ protofilaments. Proteins 61, 1101-1110
32. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
33. Geladopoulos, T. P., Sotiroudis, T. G. and Evangelopoulos, A. E. (1991) A malachite green colorimetric assay for protein phosphatase activity. Anal. Biochem. 192, 112-116
34. Beuria, T. K., Krishnakumar, S. S., Sahar, S., Singh, N., Gupta, K., Meshram, M. and Panda, D. (2003) Glutamate-induced assembly of bacterial cell division protein FtsZ. J. Biol. Chem. 278, 3735-3741
35. Santra, M. K. and Panda, D. (2003) Detection of an intermediate during unfolding of bacterial cell division protein FtsZ: loss of functional properties precedes the global unfolding of FtsZ. J. Biol. Chem. 278, 21336-21343
36. Scatchard, G. (1949) The attraction of proteins for small molecules and ions. Ann. N.Y. Acad. Sci. 51, 660-672
37. 2+-mediated GTP-dependent dynamic assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro. EMBO J. 16, 5455-5463
38. Mukherjee, A. and Lutkenhaus, J. (1998) Dynamic assembly of FtsZ regulated by GTP hydrolysis. EMBO J. 17, 462-469
39. Scheffers, D. J., Blaauwen, T. d. and Driessen, A. J. M. (2000) Non-hydrolysable GTP-γ-S stabilizes the FtsZ polymer in a GDP-bound state. Mol. Microbiol. 35, 1211-1219
40. Small, E. and Adinall, S. G. (2003) Dynamic FtsZ polymerization is sensitive to the GTP to GDP ratio and can be maintained at a steady state using a GTP-regeneration system. Microbiology 149, 2235-2242
41. Mukherjee, A. and Lutkenhaus, A. (1999) Analysis of FtsZ assembly by light scattering and determination of the role of divalent metal cations. J. Bacteriol. 181, 823-832
42. Aggarwal, B. B., Kumar, A., Aggarwal, M. S. and Shishodia, S. (2005) Curcumin derived from turmeric (Curcuma longa): a spice for all seasons. In Phytopharmaceuticals in Cancer Chemoprevention (Preuss, H, ed.), pp. 349-387, CRC Press, Boca Raton, FL
43. Kumar, S., Dubey, K. K., Tripathi, S., Fujii, M. and Misra, K. (2000) Design and synthesis of curcumin-bioconjugates to improve systemic delivery. Nucleic Acids Symp. Ser. 44, 75-76