Targeted disruption of a G protein α subunit gene results in reduced growth and pathogenicity in Rhizoctonia solani

World Journal of Microbiology and Biotechnology

Volumn 24, Issue 3, 2008, Pages 345-351

  Charoensopharat, Kanlayani ^a   Aukkanit, Nuntipa ^a   Thanonkeo, Sudarat ^b   Saksirirat, Weerasak ^a   Thanonkeo, Pornthap ^a   Akiyama, Kouichi ^c  

^a KHON KAEN UNIVERSITY   (Thailand)

^b MAHASARAKHAM UNIVERSITY   (Thailand)

^c EHIME UNIVERSITY   (Japan)

Author keywords

G protein subunit; Gene disruption; Pathogenicity; Rhizoctonia solani; Rice sheath blight disease

Indexed keywords

FUNGI; GENES; GROWTH KINETICS; SIGNAL TRANSDUCTION;

COLONY MORPHOLOGY; GENE DISRUPTION; PATHOGENICITY; RHIZOCTONIA SOLANI; RICE SHEATH BLIGHT DISEASE;

PROTEINS;

FUNGI; HYPHOMYCETES; THANATEPHORUS CUCUMERIS;

EID: 39649119892     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-007-9476-6     Document Type: Article

References (29)

1. Alspaugh JA, Perfect JR, Heitman J (1997) Cryptococcus neoformans mating and virulence are regulated by the G-protein α subunit GPA1 and cAMP. Genes Dev 11:3206-3217
2. Aukkanit N, Loonphome S, Thanonkeo S, Akiyama K, Khaiprapai P, Saksirirat W, Thanonkeo P (2004) Molecular cloning of G protein α subunit gene in Rhizoctonia solani. In: Abstracts of the 15th annual meeting of the Thai Society for Biotechnology on "Sustainable Development of SMEs through Biotechnology" and JSPS-NRCT symposium "The Forefront of Bioinformatics Application", Chiang Mai, Thailand, 132, 3-6 February 2004
3. Bölker M (1998) Sex and crime: heterotrimeric G proteins in fungal mating and pathogenesis. Fungal Genet Biol 25:143-156
4. Buss JE, Mumby SM, Casey PJ, Gilman AG, Sefton BM (1987) Myristoylated α subunits of guanine nucleotide-binding regulatory proteins. Proc Natl Acad Sci USA 84:7493-7497
5. Fang EGC, Dean RA (2000) Site-directed mutagenesis of the MagB gene affects growth and development in Magnaporthe grisea. Mol Plant Microbe Interact 13:1214-1227
6. Gao S, Nuss DL (1996) Distinct roles for two G protein α subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption. Proc Natl Acad Sci USA 93:14122-14127
7. Gronover CS, Kasulke D, Tudzynski P, Tudzynski B (2001) The role of G protein alpha subunits in the infection process of the gray mold fungus Botrytis cinerea. Mol Plant Microbe Interact 14:1293-1302
8. Hamm HE (1998) The many faces of G protein signaling. J Biol Chem 273:669-672
9. Horwitz BA, Sharon A, Lu SW, Ritter V, Sandrock TM, Yoder OC, Turgeon BG (1999) A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation. Fungal Genet Biol 26:19-32
10. i homologue gna-1 controls multiple differentiation pathways in Neurospora crassa. Mol Biol Cell 7:1283-1297
11. Jain S, Akiyama K, Mae K, Ohguchi T, Takata R (2002) Targeted disruption of a G protein α subunit gene results in reduced pathogenicity in Fusarium oxysporum. Curr Genet 41:407-413
12. Jain S, Akiyama K, Takata R, Ohguchi T (2005) Signaling via the G protein α subunit FGA2 is necessary for pathogenesis in Fusarium oxysporum. FEMS Microbiol Lett 243:165-172
13. Liu S, Dean RA (1997) G protein α subunit genes control growth, development, and pathogenicity of Magnaporthe grisea. Mol Plant Microbe Interact 10:1075-1086
14. Mazzola M (1997) Identification and pathogenicity of Rhizoctonia spp. isolated from apple roots and orchard soils. Phytopathology 87:582-587
15. Meza-carmen V, Garcia-Soto J, Ongay-Larios L, Coria R, Pedraza-Reyes M, Arnau J, Reyna-Lopez G, Martinez-Cadena G (2006) Molecular characterization of a G protein alpha-subunit-encoding gene from Mucor circinelloides. Can J Microbiol 52:627-635
16. Neer EJ (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80:249-257
17. Regenfelder E, Spellig T, Hartmann A, Lauenstein S, Bölker M, Kahmann R (1997) G proteins in Ustilago maydis: transmission of multiple signals. EMBO J 16:1934-1942
18. Robinson HL, Deacon JM (2001) Protoplast preparation and transformation of Rhizoctonia solani. Mycol Res 105:1295-1303
19. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
20. Shiotani H, Tsuge T (1995) Efficient gene targeting in the filamentous fungus Alternaria alternata. Mol Gen Genet 248:142-150
21. Simon MI, Strathmann MP, Gautam N (1991) Diversity of G proteins in signal transduction. Science 252:802-808
22. Singh A, Rohilla R, Singh US, Savary S, Willocquet L, Duveiller E (2002) An improved inoculation technique for sheath blight of rice caused by Rhizoctonia solani. Can J Plant Pathol 24:65-68
23. Truesdell GM, Yang Z, Dickman MB (2000) A Gα subunit gene from phytopathogenic fungus Colletotrichum trifolii is required for conidial germination. Physiol Mol Plant Pathol 56:131-140
24. Turgeon BG, Garber RC, Yoder OC (1987) Development of a fungal transformation system based on selection of sequences with promoter activity. Mol Cell Biol 7:3297-3305
25. i family. J Biol Chem 268:14805-14811
26. West REJ, Moss J, Vaughan M, Liu T, Liu TY (1985) Pertussis toxin-catalysed ADP-ribosylation of transducin, Cysteine 347 is the ADP-ribose acceptor site. J Biol Chem 260:14428-14430
27. Wyss P, Boller T, Wiemken A (1991) Phytoalexin response is elicited by a pathogen (Rhizoctonia solani) but not by a mycorrhizal fungus (Glomus mosseae) in soybean roots. Experientia 47:395-399
28. Yamagishi D, Otani H, Kodama M (2006) G protein signaling mediates developmental processes and pathogenesis of Alternaria alternata. Mol Plant Microbe Interact 19:1280-1288
29. Yu JH, Wieser J, Adams TH (1996) The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. EMBO J 15:5184-5190