Molecular characterization of a G protein α-subunit-encoding gene from Mucor circinelloides

Canadian Journal of Microbiology

Volumn 52, Issue 7, 2006, Pages 627-635

  Meza Carmen, Victor ^a   García Soto, Jesús ^a   Ongay Larios, Laura ^b   Coria, Roberto ^b   Pedraza Reyes, Mario ^a   Arnau, José ^c   Reyna Lopez, Georgina ^a   Martínez Cadena, Guadalupe ^a  

^a UNIVERSITY OF GUANAJUATO   (Mexico)

^b INSTITUTO DE FISIOLOGÍA CELULAR   (Mexico)

^c Biotechnological Institute Denmark   (Denmark)

Author keywords

G subunit encoding genes; Gpa3 recombinant protein; Mucor circinelloides

Indexed keywords

ELECTROPHORETIC MOBILITY; GΑ-SUBUNIT-ENCODING GENES; GPA3 RECOMBINANT PROTEIN; MUCOR CIRCINELLOIDES;

ANTIBODIES; DNA; ESCHERICHIA COLI; FUNGI; GENES; TOXIC MATERIALS;

PROTEINS;

CHOLERA TOXIN; COMPLEMENTARY DNA; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; HISTIDINE; MESSENGER RNA; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANALYTICAL METHOD; FUNGUS; GENE; GROWTH RATE; MOLECULAR ANALYSIS; PROTEIN;

ARTICLE; ASCOMYCETES; BASIDIOMYCETES; CELL GROWTH; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; ESCHERICHIA COLI; FUNGAL GENE; FUNGAL STRAIN; GENE; GENE ISOLATION; GENETIC ANALYSIS; GPA1 GENE; GPA2 GENE; GPA3 GENE; GPA4 GENE; MOLECULAR CLONING; MUCOR; MUCOR CIRCINELLOIDES; MYCELIUM; NONHUMAN; NORTHERN BLOTTING; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; YEAST; YEAST CELL; ZYGOMYCETES;

DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FUNGAL PROTEINS; GTP-BINDING PROTEIN ALPHA SUBUNITS; MOLECULAR SEQUENCE DATA; MUCOR; PHYLOGENY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI; FUNGI; MUCOR CIRCINELLOIDES;

EID: 33749524399     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W06-010     Document Type: Article

References (26)

1. Balance, D.J. 1990. Transformation systems for filamentous fungi and an overview of fungal gene structure. In Molecular industrial mycology. Edited by S.A. Leong and R.M. Berka. Marcel Dekker Inc., New York. pp. 1-29.
2. Bartnicki-Garcia, S. 1963. Symposium on biochemical bases of morphogenesis in fungi. III. Mold-yeast dimorphism of Mucor. Bacteriol. Rev. 27: 293-304.
3. Bartnicki-Garcia, S., and Nickerson, W.J. 1962. Nutrition, growth and morphogenesis of Mucor rouxii. J. Bacteriol. 84: 841-858.
4. Bölker, M. 1998. Sex and crime: heterotrimeric G proteins in fungal mating and pathogenesis. Fungal Genet. Biol. 25: 143-156.
5. Buss, J.E., Mumby, S.M., Casey, P.J., Gilman, A.G., and Sefton, B.N. 1987. Myryostyolated α subunits of guanine nucleotide-binding regulatory proteins. Proc. Natl. Acad. Sci. U.S.A. 84: 7493-7497.
6. Casale, W.L., McConnell, D.G., Wang, S.Y., Lee, Y.J., and Linz, J.E. 1990. Expression of a gene family in the dimorphic fungus Mucor racemosus which exhibits striking similarity to human ras genes. Mol. Cell Biol. 10: 6654-6663.
7. Chomczynski, P., and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
8. Cuellar-Mata, P., Martínez-Cadena, G., Castellano, L.E., Aldana-Veloz, G., Novoa-Martínez, G., Vargas, I., Darszon, A., and García-Soto, J. 1995. Multiple GTP-binding proteins in sea urchin sperm: evidence for Gs and small G-proteins. Dev. Growth Differ. 37: 173-181.
9. + uptake. Biochim. Biophys. Acta, 944: 1-12.
10. Gilman, A.G. 1987. G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56: 615-649.
11. Horwitz, B.A., Sharon, A., Lu, S.W., Ritter, V., Sandrock, T.M., Yoder, O.C., and Turgeon, B.G. 1999. A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation. Fungal Genet. Biol. 26: 19-32.
12. Kaziro, Y., Itoh, H., Kozasa, T., Nakafuku, M., and Satoh, T. 1991. Structure and function of signal-transducing GTP-binding proteins. Annu. Rev. Biochem. 60: 349-400.
13. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
14. s is present in dormant spores and expressed differentially during spore germination of the fungus Phycomyces blakesleeanus. Microbiology (Reading, U.K.), 141: 3149-3154.
15. Orlowski, M. 1991. Mucor dimorphism. Microbiol. Rev. 55: 234-258.
16. Raeder, U., and Brodar, P. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1: 17-20.
17. Roze, L.V., Mahanti, N., Mehigh, R., McConnell, D.G., and Linz, J.E. 1999. Evidence that MRas1 and MRas3 proteins are associated with distinct cellular functions during growth and morphogenesis in the fungus Mucor racemosus. Fungal Genet. Biol. 28: 171-189.
18. Salcedo-Hernandez, R., and Ruiz-Herrera, J. 1993. Isolation and characterization of a mycelial cytochrome aa3 deficient mutant, and the role of mitochondria in dimorphism of Mucor rouxii. Exp. Mycol. 17: 142-154.
19. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, N.Y.
20. Seo, H.S., Cho, C.H., Lee, S.Y., Cho, M.J., and Bahk, J.D. 1997. Biochemical characteristics of a rice (Oryza sativa L., IR36) G-protein α-subunit expressed in Escherichia coli. Biochem. J. 324: 273-281.
21. Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
22. Towbin, H., Staehelin, T., and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354.
23. i family. J. Biol. Chem. 268: 14805-14811.
24. Van Heeswijk, R., and Roncero, M.I.G. 1984. High frequency transformation of Mucor with recombinant plasmid DNA. Carlsberg Res. Commun. 48: 691-702.
25. West, R.E., Jr., Moss, J., Vaughan, M., Liu, T., and Liu, T.Y. 1985. Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site. J. Biol. Chem. 260: 14428-14430.
26. Wolff, A.M., Appel, K.P., Peterson, J.B., Poulsen, U., and Arnau, J. 2002. Identification and analysis of genes involved in the control of dimorphism in Mucor circinelloides (syn. racemosus). FEMS Yeast Res. 2: 203-213.