Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts

Electrophoresis

Volumn 29, Issue 3, 2008, Pages 695-705

  Han, Mee Jung ^a,b   Herlyn, Meenhard ^b   Fisher, Aron B ^c   Speicher, David W ^b  

^a BioProcess Engineering Research Center   (South Korea)

^b WISTAR INSTITUTE   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

2 D difference in gel electrophoresis; Complex proteomes; Gel based proteomic methods; Microscale solution IEF; Sample prefractionation

Indexed keywords

DEPTH PROFILING; ELECTROPHORESIS; GELS; MAMMALS; TISSUE;

2-D DIFFERENCE IN GEL ELECTROPHORESIS; COMPLEX PROTEOMES; GEL ELECTROPHORESIS; GEL-BASED; GEL-BASED PROTEOMIC METHOD; MICROSCALE SOLUTION IEF; PREFRACTIONATION; PROTEOMICS METHODS; SAMPLE PREFRACTIONATION; TISSUE EXTRACTS;

PROTEINS;

ISOPROTEIN; PROTEOME; TISSUE EXTRACT;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLUORESCENCE; FRACTIONATION; HUMAN; HUMAN CELL; ISOELECTRIC FOCUSING; LUNG PARENCHYMA; MAMMAL CELL; MELANOMA CELL; NONHUMAN; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 39449112983     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200700337     Document Type: Article

References (35)

1. Klose, J., Humangenetik 1975, 26, 231-243.
2. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
3. Miklos, G. L., Rubin, G. M., Cell 1996, 86, 521-529.
4. Godley, A. A., Packer N. H., in: Wilkins, M. R., Williams, K. L., Appel, R. D., Hochestrasser, D. F. (Eds.), Proteome Research: New Frontiers in Functional Genomics, Springer, Berlin 1997, pp. 65-91.
5. Ünlü, M., Morgan, M. E., Minden, J. S., Electrophoresis 1997, 18, 2071-2077.
6. Hanlon, W. A., Griffin, P. R., in: Speicher, D. W. (Ed.), Proteome Analysis: Interpreting the Genome, Elsevier Science, New York 2004, pp. 75-91.
7. Yan, J. X., Devenish, A. T., Wait, R., Stone, T. et al., Proteomics 2002, 2, 1682-1698.
8. Hu, Y., Wang, G., Chen, G. Y., Fu, X., Yao, S. Q., Electrophoresis 2003, 24, 1458-1470.
9. Tonge, R., Shaw, J., Middleton, B., Rowlinson, R. et al., Proteomics 2001, 1, 377-396.
10. Skynner, H.A., Rosahl, T.W., Knowles, M. R., Salim, K. et al., Proteomics 2002, 2, 1018-1025.
11. Van den Bergh, G., Clerens, S., Cnops, L., Vandesande, F., Arckens, L., J. Neurochem. 2003, 85, 193-205.
12. Van den Bergh, G., Clerens, S., Vandesande, F., Arckens, L., Electrophoresis 2003, 24, 1471-1481.
13. Zhou, G., Li, H., DeCamp, D., Chen, S. et al., Mol. Cell. Proteomics 117-124.
14. Gharbi, S., Gaffney, P., Yang, A., Zvelebil, M. J. et al., Mol. Cell. Proteomics 2002, 1, 91-98.
15. Friedman, D. B., Hill, S., Keller, J. W., Merchant, N. B. et al., Proteomics 2004, 4, 793-811.
16. Wildgruber, R., Harder, A., Obermaier, C., Boguth, G. et al., Electrophoresis 2000, 21, 2610-2616.
17. Corthals, G. L., Wasinger, V. C., Hochstrasser, D. F., Sanchez, J., Electrophoresis 2000, 21, 1104-1115.
18. Poznanović, S., Wozny, W., Schwall, G. R, Sastri, C. et al., J. Proteome Res. 2005, 4, 2117-2125.
19. Zuo, X., Echan, L., Hembach, P., Tang, H. Y. et al., Electrophoresis 2001, 22, 1603-1615.
20. Zuo, X., Speicher, D. W., Proteomics 2002, 2, 58-68.
21. Huber, L. A., Pasquali, C., Gagescu, R., Zuk, A. et al., Electrophoresis 1996, 17, 1734-1740.
22. Molloy, M. P,, Herbert, B. R., Walsh, B. J., Tyler, M. I. et al., Electrophoresisn 1998, 19, 837-844.
23. Fountoulakis, M., Takacs, M. F., Berndt, P., Langan, H., Takacs, B., Electrophoresis 1999, 20, 2181-2195.
24. Herbert, B., Righetti, P. G., Electrophoresis 2000, 21, 3639-3648.
25. Righetti, P. G., Castagna, A., Herbert, B., Anal. Chem. 2001, 73, 320-326.
26. Juhasz, I., Albelda, S. M., Elder, D. E., Murphy, G. F. et al., Am. J. Pathol. 1993, 143, 528-537.
27. Tang, Y., Feinstein, S. I., Manevich, Y., Ho, Y S., Fisher, A. B., Free Radic. Biol. Med. 2004, 37, 1736-1743.
28. Tang, H. Y., Ali-Khan, N., Echan, L. A., Levenkova, N. et al., Proteomics 2005, 5, 3329-3342.
29. Bernard, K., Litman, E., Fitzpatrick, J. L., Shellman, Y. G. et al., Cancer Res. 2003, 63, 6716-6725.
30. de Souza, G. A., Godoy, L. M., Teixeira, V. R., Otake, A. H. et al., Proteomics 2006, 6, 1460-1470.
31. Sinha, P., Kohl, S., Fischer, J., Hütter, G. et al., Electrophoresis 2000, 21, 3048-3057.
32. Sinha, P., Poland, J., Kohl, S., Schnölzer, M. et al., Electrophoresis 2003, 24, 2386-2404.
33. Jensen, O. N., Curr. Opin. Chem. Biol. 2004, 8,33-41.
34. Barbour, K. W., Lee, F., Brannan, C., Flotte, T. R. et al,, Genomics 2002, 80, 515-522.
35. Speicher, D. W., Lee, K., Tang, H. Y., Echan, L., in: Ashcroft, A. E., Brenton, G., Monaghan, J. J. (Eds.), Advances in Mass Spectrometry, Elsevier Science, New York 2004, pp. 37-57.