Co-expression of bacterial hemoglobin overrides high glucose-induced repression of foreign protein expression in Escherichia coli W3110

Biotechnology Letters

Volumn 26, Issue 14, 2004, Pages 1173-1178

  Kim, Yeon Kyu ^a   Kang, Dong Gyun ^a   Choi, Suk Soon ^b   Kim, Ji Hyeon ^c   Chung, Jae Chun ^d   Cha, Hyung Joon ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Semyung University   (South Korea)

^c Dongguk University   (South Korea)

^d Yonsei University Wonju   (South Korea)

Author keywords

catabolite repression; green fluorescent protein; hemoglobin; organophosphorous hydrolase

Indexed keywords

ARYLDIALKYLPHOSPHATASE; BACTERIAL PROTEIN; GLUCOSE; GREEN FLUORESCENT PROTEIN; HEMOGLOBIN; HEMOGLOBIN PROTEIN, VITREOSCILLA; OXYGEN; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; BIOTECHNOLOGY; CELL CULTURE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; METABOLISM; METHODOLOGY; PLASMID; PROMOTER REGION; TIME;

ARYLDIALKYLPHOSPHATASE; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; CELLS, CULTURED; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLUCOSE; GREEN FLUORESCENT PROTEINS; HEMOGLOBINS; OXYGEN; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; TIME FACTORS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VITREOSCILLA;

EID: 3943093344     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000035491.64912.84     Document Type: Article

References (24)

1. Akesson M, Hagander P, Axelsson JP (2001) Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding. Biotechnol. Bioeng. 73: 223-230.
2. Bauer KA, Bassat AB, Sawson M, Puente VT, Neway JO (1990) Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant. Appl. Environ. Microbiol. 56: 1296-1302.
3. Brosius J, Erfle M, Storella J (1985) Spacing of the -10 and -35 regions in the tac promoter: effect on its in vivo activity. J. Biol. Chem. 260: 3539-3541.
4. Caldwell SR, Newcomb JR, Schlecht KA, Raushel FM (1991) Limits of diffusion in the hydrolysis of substrates by the phophotriesterase from Pseudomonas diminuta. Biochem. 30: 7438-7444.
5. Cha HJ, Wu CF, Valdes JJ, Rao G, Bentley WE (2000) Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia coli: monitoring protein expression and solubility. Biotechnol. Bioeng. 67: 565-574.
6. de Boer HA, Comstock LJ, Yasser M (1983) The tac promoter: a functional hybrid derived from the tip and lac promoters. Proc. Nat. Acad. Sci. USA 80: 21-25.
7. Dikshit KL, Webster DA (1988) Cloning, characterization and expression of the bacterial hemoglobin gene from Vitreoscilla in Escherichia coli. Gene 70: 377-386.
8. Donarski WJ, Dumas DP, Heitmeyer DP, Lewis VE, Raushel FM (1989) Structure-activity relationship in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta. Biochem. 28: 4650-1655.
9. - recombinant Escherichia coli: some observations. Biotechnol. Prog. 15: 640-645.
10. Grimsley JK, Scholtz JM, Pace CN, Wild JR (1997) Organophosphorus hydrolase is a remarkably stable enzyme that unfolds through a homodimeric intermediate. Biochem. 36: 14366-14374.
11. Hahm DH, Pan JG, Rhee JS (1994) Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase. Biotechnol. Bioeng. 42: 100-107.
12. Han SJ, Chang HN, Lee J (1998) Fed-batch cultivation of an oxygen-dependent inducible promoter system, the nar promoter in Escherichia coli with an inactivated nar operon. Biotechnol. Bioeng. 59: 400-406.
13. Holms WH (1986) The central metabolic pathways in Eschericchia coli: relationship between flux and control at a branchpoint, efficiency of conversion to biomass, and excretion of acetate. Curr. Topic. Cell. Regul. 28: 69-105.
14. Kallio PT, Bailey JE (1996) Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of α-amylase and neutral protease in Bacillus subtilis. Biotechnol. Prog. 12: 31-39.
15. Kang DG, Kim JYH, Cha HJ (2002a) Enhanced detoxification of organophosphates using recombinant Escherichia coli with co-expression of organophosphorus hydrolase and bacterial hemoglobin. Biotechnol. Lett. 24: 879-883.
16. Kang DG, Kim YK, Cha HJ (2002b) Comparison of green fluorescent protein expression in two industrial Escherichia coli strains, BL21 and W3110, under co-expression of bacterial hemoglobin. Appl. Microbiol. Biotechnol. 59: 523-528.
17. Khosla C, Bailey JE (1988) Heterologous expression of a bacterial hemoglobin improves the growth properties of recombinant Escherichia coli. Nature 331: 633-635.
18. Lee J, Cho MH, Lee J (1996) Characterization of an oxygendependent inducible promoter system, the nar promoter, and Escherichia coli with an inactivated nar operon. Biotechnol. Bioeng. 52: 572-578.
19. Li SF, DeMoss JA (1988) Location of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL. J. Biol. Chem. 263: 13700-13705.
20. Majewski RA, Domach MM (1990) Simple constrained-optimization view of acetate overflow in E. coli. Biotechnol. Bioeng. 35: 732-738.
21. Robbins JW, Taylor KB (1989) Optimization of Escherichia coli growth by controlled addition of glucose. Biotechnol. Bioeng. 34: 1289-1294.
22. Shiloach J, Kaufman J, Guillard AS, Fass R (1996) Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (λDE3) and Escherichia coli JM109. Biotechnol. Bioeng. 49: 421-128.
23. van de Walle M, Shiloach J (1998) Proposed mechanism of acetate accumulation in two recombinant Escherichia coli strains during high density fermentation. Biotechnol. Bioeng. 57: 71-78.
24. Wu CF, Cha HJ, Rao G, Valdes JJ, Bentley WE (2000) A green fluorescent protein fusion strategy for monitoring the expression, cellular location, and separation of biologically active organophosphorus hydrolase. Appl. Microbiol. Biotechnol. 54: 78-83.