Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models

Journal of Immunological Methods

Volumn 331, Issue 1-2, 2008, Pages 59-68

  Young, Shih Houng ^a   Antonini, James M ^a   Roberts, Jenny R ^a   Erdely, Aaron D ^a   Zeidler Erdely, Patti C ^a  

^a NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH   (United States)

Author keywords

Cytokines; ELISA; Flow cytometric bead assays; Multiplex immunoassay; RT PCR

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 6; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA; ZYMOSAN;

ANIMAL EXPERIMENT; ARTICLE; CYTOMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOMODULATION; INFLAMMATION; INTERMETHOD COMPARISON; LUNG; LUNG LAVAGE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RODENT; WELDING FUME;

ANIMALS; BRONCHOALVEOLAR LAVAGE FLUID; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; LUNG; MALE; MICE; MICE, INBRED C57BL; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; ZYMOSAN;

MUS; RATTUS; RODENTIA;

EID: 39149108484     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.11.004     Document Type: Article

References (12)

1. Antonini J.M., Lawryk N.J., Murthy G.G., and Brain J.D. Effect of welding fume solubility on lung macrophage viability and function in vitro. J. Toxicol. Environ. Health, A 58 (1999) 343
2. Antonini J.M., Roberts J.R., and Clarke R.W. Strain-related differences of nonspecific respiratory defense mechanisms in rats using a pulmonary infectivity model. Inhal. Toxicol. 13 (2001) 85
3. Antonini J.M., Taylor M.D., Millecchia L., Bebout A.R., and Roberts J.R. Suppression in lung defense responses after bacterial infection in rats pretreated with different welding fumes. Toxicol. Appl. Pharmacol. 200 (2004) 206
4. Diaz M.R., Boekhout T., Theelen B., Bovers M., Cabanes F.J., and Fell J.W. Microcoding and flow cytometry as a high-throughput fungal identification system for Malassezia species. J. Med. Microbiol. 55 (2006) 1197
5. Edwards B.S., Oprea T., Prossnitz E.R., and Sklar L.A. Flow cytometry for high-throughput, high-content screening. Curr. Opin. Chem. Biol. 8 (2004) 392
6. Karlsson A.C., Martin J.N., Younger S.R., Bredt B.M., Epling L., Ronquillo R., Varma A., Deeks S.G., McCune J.M., Nixon D.F., and Sinclair E. Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells. J. Immunol. Methods 283 (2003) 141
7. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 (2001) 402
8. Rao G.V., Tinkle S., Weissman D.N., Antonini J.M., Kashon M.L., Salmen R., Battelli L.A., Willard P.A., Hoover M.D., and Hubbs A.F. Efficacy of a technique for exposing the mouse lung to particles aspirated from the pharynx. J. Toxicol. Environ. Health, A 66 (2003) 1441
9. Solano-Lopez C., Zeidler-Erdely P.C., Hubbs A.F., Reynolds S.H., Roberts J.R., Taylor M.D., Young S.H., Castranova V., and Antonini J.M. Welding fume exposure and associated inflammatory and hyperplastic changes in the lungs of tumor susceptible A/J mice. Toxicol. Pathol. 34 (2006) 364
10. Stemme V., Rymo L., Risberg B., and Stemme S. Quantitative analysis of specific mRNA species in minute cell samples by RT-PCR and flow cytometry. J. Immunol. Methods 249 (2001) 223
11. Young S.-H., Roberts J.R., and Antonini J.M. Pulmonary exposure to 1→3-beta-glucan alters adaptive immune responses in rats. Inhal. Toxicol. 18 (2006) 865
12. Young S.-H., Robinson V.A., Barger M., Porter D.W., Frazer D.G., and Castranova V. Acute inflammation and recovery in rats after intratracheal instillation of a 1→3-beta-glucan (zymosan A). J. Toxicol. Environ. Health, Part A 64 (2001) 311