Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy

International Journal of Molecular Medicine

Volumn 18, Issue 4, 2006, Pages 673-678

  Kaito, Masahiko ^a   Watanabe, Shozo ^b   Tanaka, Hideaki ^a   Fujita, Naoki ^a   Konishi, Masayoshi ^a   Iwasa, Motoh ^a   Kobayashi, Yoshinao ^a   Gabazza, Esteban Cesar ^a   Adachi, Yukihiko ^a   Tsukiyama Kohara, Kyoko ^c   Kohara, Michinori ^c  

^a MIE UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b MIE UNIVERSITY   (Japan)

^c TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

E1; E2; Electron microscopy; Hepatitis C virus; Virion

Indexed keywords

E1 PROTEIN, HEPATITIS C VIRUS; GLYCOPROTEIN E2, HEPATITIS C VIRUS; VIRUS ENVELOPE PROTEIN; VIRUS RNA;

ARTICLE; BLOOD; CHEMISTRY; GENETICS; HEPATITIS C VIRUS; HUMAN; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; METHODOLOGY; ULTRASTRUCTURE; VIRION;

HEPACIVIRUS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; RNA, VIRAL; VIRAL ENVELOPE PROTEINS; VIRION;

EID: 39049181134     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.18.4.673     Document Type: Article

References (41)

1. Lauer GM and Walker BD: Hepatitis C virus infection. N Engl J Med 345: 41-52, 2001.
2. Lindenbach BD and Rice CM: Flaviviridae: the viruses and their replication. In: Fields Virology. Knipe DM and Howley PM (eds). Lippincott Williams & Wilkins, Philadelphia, pp991-1042, 2001.
3. Alter MJ: Epidemiology of hepatitis C. Hepatology 26 (Suppl 1): 62-65, 1997.
4. Montella M, Crispo A, Izzo F, et al: HCV and hepatocellular carcinoma: A case-control study in an area of hyperendemicity. Int J Mol Med 6: 571-574, 2000.
5. Fanning LJ: The Irish paradigm on the natural progression of hepatitis C virus infection: an investigation in a homogeneous patient population infected with HCV 1b. Int J Mol Med 9: 179-184, 2002.
6. Nagao Y, Tanaka K, Kobayashi K, Kumashiro R, Sata M: A cohort study of chronic liver disease in an HCV hyperendemic area of Japan: a prospective analysis for 12 years. Int J Mol Med 13: 257-265, 2002.
7. Kato N, Hijikata M, Ootsuyama Y, et al: Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A non-B hepatitis. Proc Natl Acad Sci USA 87: 9524-9528, 1990.
8. Takamizawa A, Mori C, Fuke I, et al: Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol 65: 1105-1113, 1991.
9. Reed KE and Rice CM: Overview of hepatitis C virus genome structure, polyprotein processing, and protein processing, and protein properties. Curr Top Microbiol Immunol 244: 55-84, 2000.
10. Kohara M, Tsukiyama-Kohara K, Maki N, et al: Expression and characterization of glycoprotein gp35 of hepatitis C virus using recombinant vaccinia virus. J Gen Virol 73: 2313-2318, 1992.
11. Dubuisson J, Hsu HH, Cheung RC, Greenberg HB, Russell DG and Rice CM: Formation and intracellular localization of hepatitis C virus envelope glycoprotein complexes express by recombinant vaccinia and Sindbis viruses. J Virol 68: 6147-6160, 1994.
12. Ralston R, Thudium K, Berger K, et al: Characterization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia viruses. J Virol 67: 6753-6761, 1993.
13. Kaito M, Watanabe S, Tsukiyama-Kohara K, et al: Hepatitis C virus particle detected by immunoelectron microscopic study. J Gen Virol 75: 1755-1760, 1994.
14. Watanabe S, Kaito M and Kohara M: Immunoelectron microscopic characterization of HCV. In: Methods in Molecular Medicine, vol. 19: Hepatitis C protocols. Lau JYN (ed). Humana Press, Totowa, NJ, pp279-285, 1998.
15. Fujita N, Kaito M, Ishida S, et al: Paraformaldehyde protects of Hepatitis C Virus particles during ultracentrifugation. J Med Virol 63: 108-116, 2001.
16. Kaito M, Watanabe S, Kohara M, Tsukiyama-Kohara K, Ishida S, Takeo M, Tanaka H, Horiike S, Naoki F, Iwasa M, Gabazza EC, Ikoma J and Adachi Y: Hepatitis C virus particles detected by immunogold electron microscopy. In: Science, Technology and Education of Microscopy: an Overview. Mendez-Vilas A (ed). Vol. 2, Formatex, Badajoz, pp490-498, 2003.
17. Ishida S, Kaito M, Kohara M, et al: Hepatitis C virus core particle detected by immunoelectron microscopy and optical rotation technique. Hepatol Res 20: 335-347, 2001.
18. Simmonds P, McOmish F, Yap PL, et al: Sequence variability in the 5′ non-coding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity. J Gen Virol 74: 661-668, 1993.
19. Kaneko S, Murakami S, Unoura M and Kobayashi K: Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction. J Med Virol 37: 278-282, 1992.
20. Yoshioka K, Kakumu S, Wakita T, et al: Detection of hepatitis C virus by polymerase chain reaction and response to interferon-α therapy: relationship to genotypes of hepatitis C virus. Hepatology 16: 293-299, 1992.
21. Fubahashi S, Itamura S, Iinuma H, Nerome K, Sugimoto M and Shida H: Increased expression in vivo and in vitro of foreign genes directed by A-type inclusion body hybrid promoters in recombinant vaccinia viruses. J Virol 65: 5584-5588, 1991.
22. Matsuura Y, Harada S, Suzuki R, et al: Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells. J Virol 66: 1425-1431, 1992.
23. Heller T, Saito S, Auerbach J, et al: An in vitro model of hepatitis C virion production. Proc Natl Acad Sci USA 102: 2579-2583, 2005.
24. Wakita T, Pietschmann, Kato T, et al: Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 11: 791-796, 2005.
25. Thomson M, Nascimbeni M, Gozales S, Murthy KK, Rehermann B and Liang TJ: Emergence of a distinct pattern of viral mutations in chimpanzees infected with a homogeneous inoculum of hepatitis C virus. Gastroenterology 121: 1226-1230, 2001.
26. Kato T, Furusaka A, Miyamoto M, et al: Sequence analysis of hepatitis C virus isolated from a fulminant hepatitis patient. J Med Virol 64: 334-339, 2001.
27. Deleersnyder V, Pillez A, Wychowski C, et al: Formation of native hepatitis C virus glycoprotein complexes. J Virol 71: 697-704, 1997.
28. Dubuisson J: Folding, assembly and subcellular localization of hepatitis C virus glycoproteins. Curr Top Microbiol Immunol 242: 135-148, 2000.
29. Op De Beeck A, Voisset C, Bartosch B, et al: Characterization of functional hepatitis C virus envelope glycoproteins. J Virol 78: 2994-3002, 2004.
30. Lavillette D, Bartosch B, Nourrisson D , et al: Hepatitis C virus glycoproteins mediated low pH-dependent membrane fusion with liposomes. J Biol Chem 281: 3909-3017, 2006.
31. Bartosch B, Dubuisson J and Cosset FL: Infectious hepatitis C pseudoviruses containing functional E1E2 envelope protein complexes. J Exp Med 197: 633-642, 2003.
32. Drummer HE and Poumbourios P: Hepatitis C virus glycoprotein E2 contains a membrane-proximal heptad repeat sequence that is essential for E1E2 glycoprotein heterodimerization and viral entry. J Biol Chem 279: 30066-30072, 2004.
33. Pileri P, Uematsu Y, Campagnoli S, et al: Binding of hepatitis C virus to CD81. Science 282: 938-941, 1998.
34. Yagnik AT, Lahm A, Meola A, et al: A model for the hepatitis C virus envelope glycoprotein E2. Proteins 40: 355-366, 2000.
35. Hsu M, Zhang J, Flint M, et al: Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci USA 100: 7271-7276, 2003.
36. Cormier EG, Tsamis F, Kajumo F, Durso RJ, Gardner JP and Dragic T: CD81 is an entry coreceptor for hepatitis C virus. Proc Natl Acad Sci USA 101: 7270-7274, 2004.
37. Bartosch B, Vitelli A, Granier C, et al: Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor. J Biol Chem 278: 41624-41630, 2003.
38. Zhang J, Randall G, Higginbottom A, Monk P, Rice CM and McKeaking JA: CD81 is required for hepatitis C virus glycoprotein-mediated viral infection. J Virol 78: 1448-1455, 2004. (Pubitemid 38095855)
39. Scarselli E, Ansuini H, Cerino R, et al: The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J 21: 5017-5025, 2002.
40. Agnello V, Abel G, Elfahal M, Knight GB and Zhang QX: Hepatitis C virus and other flaviviridae viruses enter cells via low density lipoprotein receptor. Proc Natl Acad Sci USA 96: 12766-12771, 1999.
41. André P, Komurian-Pradel F, Deforges S, et al: Characterization of low- and very-low-density hepatitis C virus RNA-containing particles. J Virol 76: 6919-6928, 2002.