Deuterium oxide stabilizes conformation of tubulin: A biophysical and biochemical study

Journal of Biochemistry and Molecular Biology

Volumn 41, Issue 1, 2008, Pages 62-67

  Das, Amlan ^a   Sinha, Sharmistha ^b   Acharya, Bipul R ^a   Paul, Pinaki ^a   Bhattacharyya, Bhabatarak ^c   Chakrabarti, Gopal ^a  

^a UNIVERSITY OF CALCUTTA   (India)

^b INDIAN INSTITUTE OF SCIENCE   (India)

^c BOSE INSTITUTE   (India)

Author keywords

Circular dichroism; Deuterium oxide; Differential scanning calorimeter; Fluorescence; Protein conformational stabilization; Tubulin

Indexed keywords

EID: 39049124546     PISSN: 12258687     EISSN: 02191024     Source Type: Journal    
DOI: 10.5483/bmbrep.2008.41.1.062     Document Type: Article

References (18)

1. Henderson, R. F., Henderson, T. R. and Woodfin, B. H. (1970) Effects of D2O on the association-dissociation equilibrium in subunit proteins. J. Biol. Chem. 245, 3733-3737.
2. Tehei, M., Madern, D., Pfister, C. and Zaccal, G. (2001) Fast dynamics of halophilic malate dehydrogenase and BSA measured by neutron scattering under various solvent conditions influencing protein stability. Proc. Natl. Acad. Sci. U.S.A. 98, 14356-14361.
3. Santra, M. K., Dasgupta, D. and Panda, D. (2005) Deuterium oxide promotes assembly and bundling of FtsZ protofilaments. Proteins 61, 1101-1110.
4. Verheul, M., Roefs, S. P. F. M. and Kruif, K. G. de. (1998) Aggregation of b-lactoglobulin and influence of D2O. FEBS Lett. 421, 273-276.
5. Chellgren, B. W. and Creamer, T. P. (2004) Effects of H2O and D2O on Polyproline II Helical Structure. J. Am. Chem. Soc. 126, 14734-14735.
6. Eker, E., Griebenow, K. and Schweitzer-Stenner, R. (2003) Stable conformations of tripeptides in aqueous solution studied by UV circular dichroism spectroscopy. J. Am. Chem. Soc. 125, 8178-8185.
7. Kresheck, G. C., Schneider, H. and Scheraga, H. A. (1965) The effect of D2-O on the thermal stability of proteins. Thermodynamic parameters for the transfer of model compound from H2-O to D2-O. J. Phys. Chem. 69, 3132-3144.
8. Lemm, U. and Wenzel, M. (1981) Stabilisation of enzymes and antisera by heavy water. Eur. J. Biochem. 116, 441-445.
9. Chakrabarti, G., Kim, S., Gupta, M. L. Jr., Barton, J. S. and Himes, R. (1999) Stabilization of tubulin by deuterium oxide. Biochemistry 38, 3067-3072.
10. Lakowicz, J. R. (1999) Principles of Fluorescence Spectroscopy, Chapter 6, pp 185-210. Kluwer Academic/Plenum Publishers, New York, USA.
11. Britto, P. J., Knipling, L., Mcphie, P. and Wolff, J. (2005) Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization. Biochem. J. 389, 549-558.
12. Roychowdhury, M., Sarkar, N., Manna, T., Bhattacharyya, S., Sarkar, T., BasuSarkar, P., Roy, S. and Bhattacharyya, B. (2000) Sulfhydryls of tubulin, A probe to detect conformational changes of tubulin. Eur. J. Biochem. 276, 3469-3476.
13. Chaudhuri, A. R., Khan, I. A. and Luduena, R. F. (2001) Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach. Biochemistry 40, 8834-8841.
14. Conway, B. E. (1981) Ionic Hydration in Chemistry and Biopaysics, Studies in Physical and Theoretical Chemistry, Vol.12. Elsevier Science, Amsterdam, The Netherlands, 12.
15. Makhatadze, G. I., Clore, G. N. and Groneborn, A. N. (1995) Solvent isoptope effect and protein stability. Nat. Struct. Biol. 2, 852-855.
16. Panda, D., Chakrabarti, G., Hudson J., Pigg, K., Miller, H. P., Wilson, L. and Himes R. H. (2000) Suppression of microtubule dynamic instability and treadmilling by deuterium oxide. Biochemistry 39, 5075-5081.
17. Hamel, E. and Lin, C. (1981) Glutamate-induced polymerization of tubulin: characteristics of the reaction and application to the large-scale purification of tubulin. Arch. Biochem. Biophys. 209, 29-40.
18. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.