Comparison of phenotypic and genotypic methods for the detection of environmental isolates of Pseudomonas aeruginosa

International Journal of Hygiene and Environmental Health

Volumn 211, Issue 1-2, 2008, Pages 143-155

  Atzél, Béla ^a   Szoboszlay, Sándor ^a   Mikuska, Zsolt ^a   Kriszt, Balázs ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

API; Environmental safety; ETA gene; Exotoxin A; Hydrocarbon contaminated sites; Pathogen; PCR; Pseudomonas aeruginosa identification; Remediation technology

Indexed keywords

PROKARYOTA; PSEUDOMONAS AERUGINOSA;

EID: 39049115988     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2007.04.001     Document Type: Article

References (28)

1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., and Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25 (1997) 3389-3402
2. Chayabutra C., and Ju L.K. Degradation of n-hexadecane and its metabolites by Pseudomonas aeruginosa under microaerobic and anaerobic denitrifying conditions. Appl. Environ. Microbiol. 66 (2000) 493-498
3. Cystic Fibrosis Foundation, Patient registry 2001. Annual Data Report. Cystic Fibrosis Foundation, Bethesda, MD.
4. Diekema D.J., Pfaller M.A., and Jones R.N. Survey of blood-stream infections due to Gram-negative bacilli: frequency of occurence and antimicrobial susceptibility of isolates collected in the US, Canada and Latin America for the SENTRY Antimicrobial Surveillance Program. Clin. Infect. Dis. 29 (1999) 595-607
5. Ernst R.K., Adams K.N., Moskowitz S.M., Kraig G.M., Kawasaki K., Stead C.M., St. Trent M., and Miller S.I. The Pseudomonas aeruginosa lipid A deacylase: selection for expression and loss within the cystic fibrosis airway. J. Bacteriol. 188 (2006) 191-201
6. Ferguson M.W., Maxwell J.A., Vincent T.S., Silva J., and Olson J.C. Comparison of the exoS gene and protein expression in soil and clinical isolates of Pseudomonas aeruginosa. Infect. Immun. 69 (2001) 2198-2210
7. Gray G.L., Smith D.H., Baldridge J.S., Harkins R.N., Vasil M.L., Chen E.Y., and Heyneker H.L. Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin a structural gene of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 81 (1984) 2645-2649
8. Grobe S., Wingender J., and Truper H.G. Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems. J. Appl. Bacteriol. 79 (1995) 94-102
9. Hudson V.L., Wielinski C.L., and Regelmann W.E. Prognostic implications of initial oropharyngeal bacterial flora in patients with cystic fibrosis diagnosed before the age of two years. J. Pediatr. 122 (1993) 854-860
10. Hungarian Standard: MSZ 21470-77:1988. Environmental soil examinations.
11. Jarvis, W.R., Martone, W.J., 1992. Predominant pathogens in hospital infections. J. Antimicrob. Chemoter. Suppl. A, 19-24.
12. Joyanes P., del Carmen Conejo M., Martinez-Martinez L., and Perea E.J. Evaluation of the VITEK 2 system for the identification and susceptibility testing of three species of nonfermenting Gram-negative rods frequently isolated from clinical samples. J. Clin. Microbiol. 39 (2001) 3247-3253
13. Khan A.A., and Cerniglia E. Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl. Environ. Microbiol. 60 (1994) 3739-3745
14. Kiska D.L., Kerr A., Jones M.C., Caracciolo J.A., Eskridge B., Jordan M., Miller S., Hughes D., King N., and Gilligan P.H. Accuracy of four commercial systems for identification of Burkholderia cepacia and other Gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. J. Clin. Microbiol. 34 (1996) 886-891
15. Lu J., Perng C., Lee S., and Wan C. Use of PCR with universal primers and restriction endonuclease digestion for detection and identification of common bacterial pathogens in cerebrospinal fluid. J. Clin. Microbiol. 38 (2000) 2076-2080
16. Lyczak J.B., Cannon C.L., and Pier G.B. Lung infections associated with cystic fibrosis. Clin. Microbiol. Rev. 15 (2002) 194-222
17. Mederios A.A., Obrien T.F., Wacker W.E., and Yulug N.F. Effect of salt concentration on the apparent in vitro susceptibility of Pseudomonas and other Gram-negative bacilli to gentamicin. J. Infect. Dis. 124 Suppl. (1971) S59-S64
18. Mossel, D.A., Indacochea, L., 1971. A new cetrimide medium for the detection of Pseudomonas aeruginosa. In: Bergan, T. (Ed.), Human- and animal-pathogenic members of the genus Pseudomonas. In: Starr, M.P., Stolp, H., Trüper, H.G., Balows, A., Schlegel, H.G. (Eds.), The Prokaryotes, A Handbook of Isolation, Characteriztation and Identification of Bacteria. 1981, Springer, Berlin, Heidelberg, New York, p. 673.
19. Nikolausz M., Márialigeti K., and Kovács G. Comparison of RNA- and DNA-based species diversity investigations in rhizoplane bacteriology with respect to chloroplast sequence exclusion. J. Microbiol. Methods 56 (2004) 365-373
20. Qin X., Emerson J., Stapp J., Stapp L., Abe P., and Burns J.L. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting Gram-negative bacilli from patients with cystic fibrosis. J. Clin. Microbiol. 41 (2003) 4312-4317
21. Ribosomal DNA Database: http://www.psb.ugent.be/rRNA/primers/.
22. Saiman L., Burns J.L., Larone D., Chen Y., Garber E., and Whittier S. Evaluation of MicroScan Autoscan for identification of Pseudomonas aeruginosa isolates from cystic fibrosis patients. J. Clin. Microbiol. 41 (2003) 492-494
23. Shelly D.B., Spilker T., Gracely E., Coenye T., Vandamme P., and LiPuma J.J. Utility of commercial test systems for identification of Burkholderia cepacia complex from cystic fibrosis sputum culture. J. Clin. Microbiol. 38 (2000) 3112-3115
24. Song K.P., Chan T.K., Ji Z.L., and Wong S.W. Rapid identification of Pseudomonas aeruginosa from ocular isolates by PCR using exotoxin A-specific primers. Mol. Cell. Probes 14 (2000) 199-204
25. Spilker T., Coenye T., Vandamme P., and LiPuma J.J. PCR-based assay for differentation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cytic fibrosis patients. J. Clin. Microbiol. 42 (2004) 2074-2079
26. Sun Yoon S., Coakley R., Lau G.W., Lymar S.V., Gaston B., Karabulut A.C., Hennigan R.F., Hwang S., Buettber G., Schurr M.J., Mortensen J.E., Burns J.L., Speert D., Boucher R.C., and Hasset D.J. Anaerobic killing of mucoid Pseudomonas aeruginosa by acidified nitrite derivates under cystic fibrosis airway condition. J. Clin. Invest. 116 (2006) 436-446
27. Tang Y., Ellis N.M., Hopkins M.K., Smith D.H., Dodge D.E., and Persing D.H. Comparison of the phenotypic and genotypic techniques for identification of unusual aerobic pathogenic Gram-negative bacilli. J. Clin. Microbiol. 36 (1998) 3674-3679
28. Wellinghausen N., Köthe J., Wirths B., Sigge A., and Poppert S. Superiority of molecular techniques for identification of Gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis. J. Clin. Microbiol. 43 (2005) 4070-4075