A rapid method for gene expression analysis of Borna disease virus in neurons and astrocytes using laser microdissection and real-time RT-PCR

Journal of Virological Methods

Volumn 148, Issue 1-2, 2008, Pages 58-65

  Porombka, Doris ^a   Baumgärtner, Wolfgang ^a   Herden, Christiane ^a  

^a UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

Author keywords

Astrocyte; Borna disease virus; Immunofluorescence; Laser microdissection; Neuron; Real time RT PCR

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCOPROTEIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MATRIX PROTEIN; NUCLEOPROTEIN; SUCCINATE DEHYDROGENASE (UBIQUINONE);

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; ASTROCYTE; BORNA DISEASE VIRUS; CONTROLLED STUDY; GENE EXPRESSION; GENE SEQUENCE; INTRON; LASER MICRODISSECTION; NERVE CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; VIRUS CELL INTERACTION; VIRUS INFECTION;

ANIMALS; ASTROCYTES; BORNA DISEASE; BORNA DISEASE VIRUS; BRAIN; GENE DOSAGE; GENE EXPRESSION PROFILING; MICRODISSECTION; NEURONS; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

BORNA DISEASE VIRUS; RATTUS;

EID: 39049096103     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.10.014     Document Type: Article

References (31)

1. Bi W.L., Keller-McGandy C., Standaert D.G., and Augood S.J. Identification of nitric oxide synthase neurons for laser capture microdissection and mRNA quantification. Biotechniques 33 (2002) 1274-1283
2. Briese T., Schneemann A., Lewis A.J., Park Y.S., Kim S., Ludwig H., and Lipkin W.I. Genomic organization of Borna disease virus. Proc. Natl. Acad. Sci. 91 (1994) 4362-4366
3. Burbach G.J., Dehn D., Nagel B., Del Turco D., and Deller T. Laser microdissection of immunolabelled astrocytes allows quantification of astrocytic gene expression. J. Neurosci. Methods 138 (2004) 141-148
4. Burgemeister R., Gangnus R., Haar B., Schutze K., and Sauer U. High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology. Pathol. Res. Pract. 199 (2003) 431-436
5. Cubitt B., Oldstone C., and de la Torre J.C. Sequence and genome organization of Borna disease virus. J. Virol. 68 (1994) 1382-1396
6. de la Torre J.C. Molecular biology of Borna disease virus and persistence. Front. Biosci. 7 (2002) 569-579
7. Fend F., Emmert-Buck M.R., Chuaqui R., Cole K., Lee J., Liotta L.A., and Raffeld M. Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. Am. J. Pathol. 54 (1999) 61-66
8. Fink L., Kinfe T., Seeger W., Ermert L., Kummer W., and Bohle R.M. Immunostaining for cell picking and real-time mRNA quantification. Am. J. Pathol. 157 (2000) 1459-1466
9. Ginsberg S.D., and Che S. Combined histochemical staining, RNA amplification, regional, and single cell cDNA analysis within the hippocampus. Lab. Invest. 84 (2004) 952-962
10. Gosztonyi G., and Ludwig H. Borna disease-neuropathology and pathogenesis. Curr. Top. Microbiol. Immunol. 190 (1995) 39-74
11. Herden C., Herzog S., Richt J.A., Nessler A., Christ M., Failing K., and Frese K. Distribution of Borna disease virus in the brain of rats infected with an obesity-inducing virus strain. Brain Pathol. 10 (2000) 39-48
12. Herden C., Schluesener H., and Richt J.A. Expression of AIF-1 and HO-1 in brains of rats after experimental infection with the neurotropic Borna disease virus. Neuropathol. Appl. Neurobiol. 31 (2005) 512-521
13. Makay I.M., Arden K.E., and Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 30 (2002) 1292-1305
14. Paxinos G., and Watson C. The Rat Brain in Stereotaxic Coordinates. 4th ed. (1998), Academic Press, San Diego
15. Perez M., Sanchez A., Cubitt B., Rosario D., and de la Torre J.C. A reverse genetics system for Borna disease virus. J. Gen. Virol. 84 (2003) 3099-3104
16. Porombka D., Herzog D., Baumgärtner W., and Herden C. Preservation of RNA and destruction of infectivity in microdissected brain tissue of Lewis rats infected with the Borna disease virus. J. Virol. Methods 135 (2006) 247-253
17. Pringle C.R. Virus Taxonomy 1996-A bulletin from the Xth Internattional Congress of Virology in Jerusalem. Arch. Virol. 141 (1996) 2251-2256
18. Prosniak M., Zborek A., Scott G.S., Roy A., Phares T.W., Koprowski H., and Hooper D.C. Differential expression of growth factors at the cellular level in virus-infected brain. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 6765-6770
19. Richt J.A., Herzog S., Schmeel A., Frese K., Clements J.E., and Rott R. Current knowledge about Borna disease and its causative agent. In: Nakajima H., and Plowright W. (Eds). Equine Infectious Diseases 7 (1995), R&W Publication Ltd., Newmarket 55-59
20. Richt J.A., Pfeuffer I., Christ M., Frese K., Bechter K., and Herzog S. Borna disease virus infection in animals and humans. Emerg. Infect. Dis. 3 (1997) 343-352
21. Richt J.A., Grabner A., Herzog S., Garten W., and Herden C. Borna disease in horses. In: Sellon D., and Long M. (Eds). Equine Infectious diseases (2007), Elsevier, USA 207-213 (Chapter 22)
22. Rott R., and Becht H. Natural and experimental Borna disease in animals. Curr. Top. Microbiol. Immunol. 190 (1995) 17-30
23. Schaudien D., Baumgärtner W., and Herden C. High preservation of DNA standards diluted in 50% glycerol. Diagn. Mol. Pathol. 16 (2007) 153-157
24. Schneider P.A., Schneemann A., and Lipkin W.I. RNA splicing in Borna disease virus, a nonsegmented, negative-strand RNA virus. J. Virol. 68 (1994) 5007-5012
25. Schneider U., Naegele M., and Staeheli P. Active Borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphate ratio but no viral X protein. J. Virol. 77 (2003) 1171-1179
26. Schütze K., and Lahr G. Identification of expressed genes by laser-mediated manipulation of single cells. Nat. Biotechnol. 16 (1998) 737-742
27. Staeheli P., Sauder C., Hausmann J., Ehrensperger F., and Schwemmle M. Epidemiology of Borna disease virus. J. Gen. Virol. 81 (2000) 2123-2135
28. Tomonaga K., Kobayashi T., and Ikuta K. Molecular and cellular biology of Borna disease virus infection. Microbes Infect. 4 (2002) 491-500
29. Vandesompele J., De Preter K., Patty F., Poppe B., Van Roy N., De Paepe A., and Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 (2002) (Research0034. Epub.)
30. von Smolinski D., Leverkoehne I., von Samson-Himmelstjerna G., and Gruber A.D. Impact of formalin-fixation and paraffin-embedding on the ratio between mRNA copy numbers of differently expressed genes. Histochem. Cell Biol. 124 (2005) 177-188
31. Wehner T., Ruppert A., Herden C., Frese K., Becht H., and Richt J.A. Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues. J. Gen. Virol. 78 (1997) 2459-2466