Automated In-Line Gel Filtration for Native State Mass Spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 19, Issue 2, 2008, Pages 239-245

  Waitt, Greg M ^a   Xu, Robert ^a   Wisely, G Bruce ^a   Williams, Jon D ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IN-LINE GEL FILTRATION; PROTEIN-LIGAND COMPLEXES;

DETERGENTS; ELECTROSPRAY IONIZATION; FILTRATION; LIGANDS; MASS SPECTROMETRY; OPTIMIZATION;

PROTEINS;

BIO GEL P6 DG; LIGAND; PROTEIN; SEPHADEX; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATED IN LINE GEL FILTRATION; COLUMN CHROMATOGRAPHY; ELECTROSPRAY MASS SPECTROMETRY; FLOW RATE; GEL FILTRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NATIVE STATE MASS SPECTROMETRY; PARTICLE SIZE;

AWARDS AND PRIZES; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DRUG DESIGN; DRUG INDUSTRY; LIGANDS; MASS SPECTROMETRY; PROTEINS;

EID: 39049088435     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2007.05.008     Document Type: Article

References (27)

1. Loo J.A. Studying Noncovalent Protein Complexes by Electrospray Ionization Mass Spectrometry. Mass Spectrum. Rev 16 (1997) 1-23
2. Loo J.A. Electrospray Ionization Mass Spectrometry: A Technology for Studying Noncovalent Macromolecular Complexes. Int. J. Mass Spectrom 200 (2000) 175-186
3. Ganem B., and Henion J.D. Going Gently Into Flight: Analyzing Noncovalent Interactions by Mass Spectrometry. Bioorg. Med. Chem 11 (2003) 311-314
4. Heck A.J.R., and van den Heuvel R.H.H. Investigation of Intact Protein Complexes by Mass Spectrometry. Mass Spectrum. Rev 23 (2004) 368-385
5. Sobott F., Hernandez H., McCammon M.G., Tito M.A., and Robinson C.V. A Tandem Mass Spectrometer for Improved Transmission and Analysis of Large Macromolecular Assemblies. Anal. Chem 74 (2002) 1402-1407
6. Chernushevich I.V., and Thomson B.A. Collisional Cooling of Large Ions in Electrospray Mass Spectrometry. Anal. Chem 76 (2004) 1754-1760
7. van den Heuvel R.H.H., van Duijn E., Mazon H., Synowsky S.A., Lorenznen K., Versluis C., Brouns S.J.J., Langridge D., van der Oost J., Hoyes J., and Heck A.J.R. Improving the Performance of a Quadrupole Time-of-Flight Instrument for Macromolecular Mass Spectrometry. Anal. Chem 78 (2006) 7473-7483
8. Tito M.A., Tars K., Valegard K., Hajdu J., and Robinson C.V. Electrospray Time-of-Flight of the Intact MS2 Virus Capsid. J. Am. Chem. Soc 122 (2000) 3550-3551
9. Tahallah N., Pinkse M., Maier C.S., and Heck A.J.R. The Effect of the Source Pressure on the Abundance of Ions of Noncovalent Protein Assemblies in an Electrospray Ionization Orthogonal Time-of-Flight Instrument. Rapid Commun. Mass Spectrom 15 (2001) 596-601
10. Hofstader S.A., Sannes-Lowery K.A., Crooke S.T., Ecker D.J., Sasmor H., Manalili S., and Griffey R.H. Multiplexed Screening of Neutral Mass-Tagged RNA Targets Against Ligand Libraries with Electrospray Ionization FTICR MS: A Paradigm for High-Throughput Affinity Screening. Anal. Chem 71 (1999) 3436-3440
11. Benkstock K., Van Pelt C.K., Åkerud T., Sterling A., Edlund P.-O., and Roeradde J. Automated Nano-Electrospray Mass Spectrometry for Protein-Ligand Screening by Noncovalent Interaction Applied to Human H-FABP and A-FABP. J. Biomol. Screen 8 (2003) 247-256
12. Bitsch F., Aicholz R., Kallen J., Geisse S., Fournier B., and Schlaeppi J.-M. Identification of Natural Ligands of Retonic Acid Receptor-Related Orphan Receptor α Ligand-Binding Domain Expressed in Sf9 Cells-A Mass Spectrometry Approach. Anal. Biochem 323 (2003) 139-149
13. Potier N., Billas I.M.L., Steinmetz A., Scahaffer C., Van Dorsselaer A., Moras D., and Renaud J.-P. Using Nondenaturing Mass Spectrometry to Detect Fortuitous Ligands in Orphan Nuclear Receptors. Protein Sci 12 (2003) 725-733
14. Stehlin-Gaon C., Willmann D., Zeyer D., Sanglier S., Van Dorsselaer A., Renaud J.-P., Moras D., and Schüle R. All-trans Retinoic Acid is a Ligand for the Orphan Nuclear Receptor RORβ. Nat. Struct. Biol 10 (2003) 820-825
15. Krylova I.N., Sablin E.P., Xu R.X., Waitt G.M., MacKay J.A., Juzumiene D., Bynum J.M., Madauss K., Montana V., Lebedeva L., Suzawa M., Williams J.D., Williams S.P., Guy R.K., Thornton J.W., Flettrick R.J., Willson T.M., and Ingraham H.A. Structural Analyses Reveal Phosphatidyl Inositols as Ligands for the NR5 Orphan Receptors SF-1 and LRH-1. Cell 120 (2005) 343-355
16. Ortlund E.A., Lee Y., Solomon I.H., Hager J.M., Safi R., Choi Y., Guan Z., Tripthy A., Raetz C.R., McDonnell D.P., Moore D.D., and Redinbo M.R. Modulation of Human Nuclear Receptor LRH-1 Activity. Nat. Struct. Mol. Biol 12 (2005) 357-363
17. Li Y., Choi M., Cavey G., Daugherty J., Suino K., Kovch A., Bingham N.C., Kliewer S.A., and Xu H.E. Crystallographic Identification and Functional Characterization of Phospholipids as Ligands for the Orphan Nuclear Receptor Steroidogenic Factor-1. Mol. Cell 17 (2005) 491-502
18. Xu R.X., Lambert M.H., Wisely B.B., Warren E.N., Weinert E.E., Waitt G.M., Williams J.D., Collins J.L., Moore L.B., Willson T.M., and Moore J.T. A Structural Basis for Constitutive Activity in the Human CAR/RXRα Heterodimer. Mol. Cells 16 (2004) 919-928
19. Takats Z., Wiseman J.M., Gologan B., and Cooks R.G. Electrosonic Spray Ionization. A Gentle Technique for Generating Folded Proteins and Protein Complexes in the Gas Phase and for Studying Ion-Molecule Reactions at Atmospheric Pressure. Anal. Chem 76 (2004) 4050-4056
20. Wilm M., and Mann M. Analytical Properties of the Nanoelectrospray Ion Source. Anal. Chem 68 (1996) 1-8
21. Benkenestok K., Edlund P.-O., and Roeraade J. On-Line Microdialysis for Enhanced Resolution and Sensitivity During Electrospray Mass Spectrometry of Noncovalent Complexes and Competitive Binding Studies. Rapid Commun. Mass Spectrom 16 (2002) 2054-2059
22. Wilson D.J., and Konermann L. Ultrarapid Desalting of Protein Solutions for Electrospray Mass Spectrometry in a Microchannel Laminar Flow Device. Anal. Chem 77 (2005) 6887-6894
23. Cavanagh J., Benson L.M., Thompson R., and Naylor S. In-Line Desalting Mass Spectrometry for the Study of Noncovalent Biological Complexes. Anal. Chem 75 (2005) 2949-2954
24. Biorad, Tech Note 2068, Desalting with Bio-Gel® P-6DG Gel, Rev. B.
25. Sznaidman M.L., Haffner C.D., Maloney P.R., Fivush A., Chao E., Goreham D., Sierra M.L., LeGrumelec C., Xu H.E., Montana V.G., Lambert M.H., Willson T.M., Oliver W.R., and Sternbach D.D. Novel Selective Small Molecule Agonists for Peroxisome Proliferator-Activated Receptor δ (PPARδ)-Synthesis and Biological Activity. Bioorg. Med. Chem. Lett 13 (2003) 1517-1521
26. White W.L., Wagner C.D., Hall J.T., Chaney E.E., George B., Hofmann K., Miller L.A.D., and Williams J.D. Protein Open-Access Liquid Chromatography/Mass Spectrometry. Rapid Commun. Mass Spectrom 19 (2005) 241-249
27. Wagner C.D., Hall J.T., White W.L., Miller L.A.D., and Williams J.D. Automated Mass Correction and Data Interpretation for Protein Open-Access Liquid Chromatography-Mass Spectrometry. J. Mass Spectrom 42 (2007) 139-149