Oligosaccharide Mapping Reveals Hormone-Specific Glycosylation Patterns on Equine Gonadotropin α-Subunit Asn56

Endocrinology

Volumn 137, Issue 6, 1996, Pages 2543-2557

  Gotschall, R Russell ^a   Bousfield, George R ^a  

^a WICHITA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GONADOTROPIN;

ALPHA CHAIN; ARTICLE; CARBOHYDRATE ANALYSIS; GLYCOPROTEIN SYNTHESIS; HORMONE STRUCTURE; HORSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; ANIMALS; ASPARAGINE; CYANOGEN BROMIDE; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; GLYCOSYLATION; HORSES; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE;

EID: 0030004304     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.137.6.8641208     Document Type: Article

References (63)

1. Pierce JG, Parsons TF 1981 Glycoprotein hormones: structure and function. Annu Rev Biochem 50:465-490
2. Wu H, Lustbader JW, Liu Y, Canfield RE, Hendrickson WA 1994 Structure of human chorionic gonadotropin at 2.6 A resolution from MAD analysis of the selenomethionyl protein. Structure 2:545-558
3. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ, Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369:455-461
4. Bousfield GR, Perry WM, Ward DN 1994 Gonadotropins: chemistry and biosynthesis. In: Knobil E, Neill JD (eds) The Physiology of Reproduction. Raven Press, New York, p 1749-1792
5. Thotakura NR, Blithe DL 1995 Glycoprotein hormones: glycobiology of gonadotrophins, thyrotrophin and free α subunit. Glycobiology 5:3-10
6. Baenziger JU, Kumar S, Brodbeck RM, Smith PL, Beranek MC 1992 Circulatory half-life but not interaction with the lutropin/ chorionic gonadotropin receptor is modulated by sulfation of bovine lutropin oligosaccharides. Proc Natl Acad Sci USA 89:334-338
7. Morell AG, Gregoriadis G, Scheinberg IH, Hickman J, Ashwell G 1971 The role of sialic acid in determining the survival of glycoproteins in the circulation. J Biol Chem 246:1461-1467
8. Kalyan NK, Bahl OP 1981 Effect of deglycosylation on the subunit interactions and receptor binding activity of human chorionic gonadotropin. Biochem Biophys Res Commun 102:1246-1253
9. Rosa C, Amr S, Birken S, Wehmann R, Nisula B 1984 Effect of desialylation of human chorionic gonadotropin on its metabolic clearance rate in humans. J Clin Endocrinol Metab 59:1215-1219
10. Liu L, Southers JL, Cassels JW, Banks SM, Wehman RE, Blithe DL, Chen H, Nisula BC 1989 Structure-kinetic relationships of choriogonadotropin and related molecules. Am J Physiol 256:E721-E724
11. Governman JM, Parsons TF, Pierce JG 1982 Enzymatic deglycosylation of the subunits of chorionic gonadotropin: effects on formation of tertiary structure and biological activity. J Biol Chem 257:15059-15064
12. Keutmann HT, McIlroy PJ, Bergert ER, Ryan RJ 1983 Chemically deglycosylated human chorionic gonadotropin subunits: characterization and biological properties. Biochemistry 22:3067-3072
13. Liu WK, Young JD, Ward DN 1984 Deglycosylated ovine lutropin: preparation and characterization by in vitro binding and steroidogenesis. Mol Cell Endocrinol 37:29-39
14. Matzuk MM, Keene JL, Boime I 1989 Site specificity of the chorionic gonadotropin N-linked oligosaccharides in signal transduction. J Biol Chem 264:2409-2414
15. Bishop LA, Robertson DM, Cahir N, Schofield PR 1994 Specific roles for the asparagine-linked carbohydrate residues of recombinant human follicle stimulating hormone in receptor binding and signal transduction. J Mol Endocrinol 8:722-731
16. Flack MR, Froehlich J, Bennet AP, Anasti J, Nisula BC 1994 Site-directed mutagenesis defines the individual roles of the glycosylation sites on follicle-stimulating hormone. J Biol Chem 269:14015-14020
17. Sairam MR 1983 Hormonal antagonistic properties of chemically deglycosylated human choriogonadotropin. J Biol Chem 258:445-449
18. Kalyan NK, Bahl OP 1983 Role of carbohydrate in human chorionic gonadotropin: effect of deglycosylation on the subunit interaction and on its in vitro and in vivo biological properties. J Biol Chem 258:67-74
19. Calvo FO, Ryan RJ 1985 Inhibition of adenylyl cyclase activity in rat corpora luteal tissue by glycopeptides of human chorionic gonadotropin and the α-subunit of human chorionic gonadotropin. Biochemistry 24:1953-1959
20. 4-(N-acetyl-β-D-glucosaminyl)asparagine amidase PNGase F. J Mol Biol 241:622-623
21. Plummer TH, Elder JH, Alexander S, Phelan AW, Tarentino AL 1984 Demonstration of peptide N-glycosidase F activity in endo-β-N-acetylglucosaminidase F preparations. J Biol Chem 259:10700-10704
22. Tarentino AL, Gómez CM, Plummer TH, Jr 1985 Deglycosylation of asparagine-linked glycans by peptide-N-glycosidase F. Biochemistry 24:4665-4671
23. Van Zuylen CWEM, De Beer T, Rademaker GJ, Haverkamp J, Thomas-Oates JE, Hard K, Kamerling JP, Vliegenthart FG 1995 Site-specific and complete enzymatic deglycosylation of the native human chorionic gonadotropin α-subunit. Eur J Biochem 231:754-760
24. Ronin C, Papandreou M-J, Canonne C, Weintraub BD 1987 Carbohydrate chains of human thyrotropin are differentially susceptible to endoglycosidase removal on combined and free polypeptide subunits. Biochemistry 26:5848-5853
25. Miura Y, Perkel VS, Magner JA 1988 Differential susceptibility to N-glycanase at the individual glycosylation sites of mouse thyrotropin and free α-subunits. Endocrinology 123:2207-2213
26. Weisshaar G, Hiyama J, Renwick AGC 1991 Site-specific N-gly-cosylation of human chorionic gonadotropin - structural analysis of glycopeptides by one- and two-dimensional 1H NMR spectroscopy. Glycobiology 1:393-404
27. Weisshaar G, Hiyama J, Renwick AGC, Nimtz M 1991 NMR investigations of the N-linked oligosaccharides at individual glycosylation sites of human lutropin. Eur J Biochem 195:257-268
28. Weisshaar G, Hiyama J, Renwick AGC 1990 Site-specific N-gly-cosylation of ovine lutropin: structural analysis by one- and two-dimensional 1H-NMR spectroscopy. Eur J Biochem 192:741-751
29. Hardy MR, Townsend RR 1988 Separation of positional isomers of oligosaccharides and glycopeptides by high-performance anion-exchange chromatography with pulsed amperometric detection. Proc Natl Acad Sci USA 85:3289-3293
30. Cole HH, Pencharz RI, Goss H 1946 On the biological properties of highly purified gonadotropin from pregnant mare serum. Endocrinology 27:548-553
31. Murphy BD, Martinuk SD 1991 Equine chorionic gonadotropin. Endocr Rev 12:27-44
32. Liu W-K, Bousfield GR, Moore WT, Jr, Ward DN 1985 Priming procedure and hormone preparations influence rat granulosa cell response. Endocrinology 116:1454-1459
33. Roser JF, Carrick FN, Papkoff H 1986 Properties of equine luteinizing hormone alpha subunit alone and in combination with various subunits. Biol Reprod 35:493-500
34. Bousfield GR, Ward DN 1984 Purification of lutropin and follitropin in high yield from horse pituitary glands. J Biol Chem 259: 1911-1921
35. Moore WT, Jr, Ward DN 1980 Pregnant mare serum gonadotropin: rapid isolation procedures for the purification of intact hormone and isolation of subunits. J Biol Chem 255:6923-6929
36. Bousfield GR, Ward DN 1992 Reduction and reoxidation of equine gonadotropin α subunits. Endocrinology 131:2986-2998
37. Laemmli UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
38. Gooley AA, Classon BJ, Marschalek R, Williams KL 1991 Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the detection of Xaa-Pro-Xaa-Xaa as a motif for Thr O-glycosylation. Biochem Biophys Res Commun 178:1194-1201
39. Hardy MR, Townsend RR, Lee YC 1988 Monosaccharide analysis of glycoconjugates by anion exchange chromatograpy with pulsed amperometric detection. Anal Biochem 170:54-62
40. Blithe DL 1990 Carbohydrate composition of the α-subunit of human choriogonadotropin (hCGα) and the tree α molecules produced in pregnancy: most free α and some combined hCGα molecules are fucosylated. Endocrinology 126:2788-2799
41. Ward DN, Bousfield GR, Gordon WL, Sugino H 1989 Chemistry of the peptide components of glycoprotein hormones. In: Keel BA, Grotjan Jr HE (eds) Microheterogeneity of Glycoprotein Hormones. CRC Press, Boca Raton, pp 1-21
42. Matsui T, Mizuochi T, Titani K, Okinaga T, Hoshi M, Bousfield GR, Sugino H, Ward DN 1994 Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin β-subunits. Biochemistry 33:14039-14048
43. Damm JBL, Hard K, Kammerling JP, van Dedem GWK, Vliegenthart FG 1990 Structure determination of the major N- and O-linked carbohydrate chains of the β subunit from equine chorionic gonadotropin. Eur J Biochem 189:175-183
44. 4-4-GalNAc and siaα2,3Gal, respectively. J Biol Chem 268:795-802
45. Baenziger JU, Green ED 1988 Pituitary glycoprotein hormone oligosaccharides: structure, synthesis and function of the asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin. Bioch Biophys Acta 947:287-306
46. Rohrer JS 1995 Separation of asparagine-linked oligosaccharides by high-pH anion-exchange chromatography with pulsed amperometric detection: empirical relationships between oligosaccharide structure and chromatographic retention. Glycobiology 5:359-363
47. Lee K-O, Gesundheit N, Chen H-C, Weintraub BD 1986 Enzymatic deglycosylation of thryoid-stimulating hormone with peptide N-glycosidase F and endo-β-N-acetylglucosamindase F. Biochem Biophys Res Commun 138:230-237
48. Matzuk MM, Boime I 1988 Site-specific mutagenesis defines the intracellular role of the asparagine-linked oligosaccharides of chorionic gonadotropin β subunit. J Biol Chem 263:17106-17111
49. Bedows E, Norton SH, Huth JR, Suganumea N, Boime I, Ruddon RW 1994 Misfolded human chorionic gonadotropin β subunits are secreted from transfected Chinese hamster ovary cells. J Biol Chem 269:10574-10580
50. Hiyama J, Weisshaar G, Renwick AGC 1992 The asparagine-linked oligosaccharides at individual glycosylation sites in human thyrotropin. Glycobiology 2:401-409
51. Bousfield GR, Ward DN 1994 Evidence for two folding domains in glycoprotein hormone α subunits. Endocrinology 135:624-635
52. Huth JR, Perini F, Lockridge O, Bedows E, Ruddon RW 1993 Protein folding and assembly in vitro parallel intracellular folding and assembly: catalysis of folding and assembly of the human chorionic gonadotropin αβ dimer by protein disulfide isomerase. J Biol Chem 268:16472-16482
53. Parsons TF, Strickland TW, Pierce JG 1985 Disassembly and assembly of glycoprotein hormones. Methods Enzymol 109:736-749
54. Slaughter S, Wang Y, Myers RV, Moyle WR 1995 The lutropin β-subunit N-terminus facilitates subunit combination by offsetting the inhibitory effects of residues needed for LH activity. Mol Cell Endocrinol 112:21-25
55. Blithe DL, Ilies RK 1995 The role of glcosylation in regulating the glycoprotein hormone free α subunit and free β subunit combination in the extraembryonic coelomic fluid of early pregnancy. Endocrinology 136:903-910
56. Papkoff H 1974 Chemical and biological properties of the subunits of pregnant mare serum gonadotropin. Biochem Biophys Res Commun 58:397-404
57. Moore Jr WT, Ward DN 1980 Pregnant mare serum gomadotropin: an in vitro biological characterization of the lutropin-follitropin dual activity. J Biol Chem 255:6930-6936
58. Bousfield GR, Liu W-K, Ward DN 1985 Hybrids from equine LH: alpha enhances, beta diminishes activity. Mol Cell Endocrinol 40: 69-77
59. Calvo FO, Keutmann HT, Bergert ER, Ryan RJ 1986 Deglycosylated human follitropin: characterization and effects on adenosine cyclic 3′,5′-phosphate production in porcine granulosa cells. Biochemistry 25:3938-3943
60. Licht P, Gallo AB, Aggarwal BB, Farmer SW, Castelino JB, Papkoff H 1979 Biological and binding activities of equine gonadotropins and pregnant mare serum gonadotropin. J Endocrinol 83:311-322
61. Stewart F, Allen WR 1979 The binding of FSH, LH, and PMSG to equine gonadal tissues. J Reprod Fertil [Suppl] 27:431-440
62. Combarnous Y, Guillou F, Martinat N 1984 Comparison of in vitro follicle-stimulating hormone (FSH) activity of equine gonadotropins (luteinizing hormone, FSH and chorionic gonadotropin) in male and female rats. Endocrinology 115:1821-1827
63. Butnev VY, Gotschall RR, Baker VL, Moore WT, Bousfield GR 1996 Negative influence of O-linked oligosaccharides of high molecular weight equine chorionic gonadotropin on its luteinizing hormone and follicle-stimulating hormone receptor-binding activities. Endocrinology 137:2530-2542