Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate-binding tag molecule

Journal of Biomolecular Techniques

Volumn 18, Issue 5, 2007, Pages 278-286

  Nakanishi, Tsuyoshi ^a   Ando, Eiji ^a   Furuta, Masaru ^a   Kinoshita, Eiji ^b,c   Kinoshita Kikuta, Emiko ^b   Koike, Tohru ^b,c   Tsunasawa, Susumu ^a,d   Nishimura, Osamu ^a,d  

^a SHIMADZU CORPORATION   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

Chemical inkjet technology; Mass spectrometry; On membrane digestion; Phosphate binding tag; Phosphoproteomics

Indexed keywords

INK; MEMBRANE PROTEIN; PHOSPHATE; PHOSPHOPEPTIDE; PHOSPHOPROTEIN; ZINC;

AMINO ACID SEQUENCE; ARTICLE; CELL MEMBRANE; CHEMISTRY; HUMAN; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PEPTIDE MAPPING; PHOSPHORYLATION; PROTEIN BINDING; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE;

AMINO ACID SEQUENCE; CELL LINE, TUMOR; CELL MEMBRANE; HUMANS; INK; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PHOSPHATES; PHOSPHOPEPTIDES; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN BINDING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; ZINC;

EID: 38949143124     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Hunter T. Protein kinase and phosphatase: The yin and yang of protein phosphorylation and signaling. Cell 1995;80:225-236.
2. Kobor MS, Greenblatt J. Regulation of transcription elongation by phosphorylation. Biochim Biophys Acta 2002;1577:261-275.
3. Mizoguchi T, Putterill J, Ohkoshi Y. Kinase and phosphatase: The cog and spring of the circadian clock. Int Rev Cytol 2006;250:47-72.
4. Johnson LN, O'Reilly M. Control by phosphorylation. Curr Opin Struct Biol 1996;6:762-769.
5. Bendt AK, Burkovski A, Schaffer S, Bott M, Farwick M, Hermann T. Towards a phosphoproteome map of Corynebacterium glutamicum. Proteomics 2003;3:1637-1646.
6. Levine A, Vannier F, Absalon C, Kuhn L, Jackson P, Scrivener E, et al. Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes. Proteomics 2006;6:2157-2173.
7. Winder SJ, Walsh MP. Smooth muscle calponin. Inhibition of actomyosin MgATPase and regulation by phosphorylation. J Biol Chem 1990;265:10,148-10,155.
8. Jaffe H, Veeranna, Shetty KT, Pant HC. Characterization of the phosphorylation sites of human high molecular weight neurofilament protein by electrospray ionization tandem mass spectrometry and database searching. Biochemistry 1998;37:3931-3940.
9. Alberts B, Bray D, Lewis K, Raff M, Roberts K, Watson JD. Molecular Biolog y of the Cell, 3rd ed., New York: Garland Publishing, 1994:195-222.
10. Thaler F, Valsasina B, Baldi R, Xie J, Stewart A, Isacchi A, et al. A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: Chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry. Anal Bioanal Chem 2003;376:366-373.
11. Posewits MC, Tempst P. Immobilized gallium(III) affinity chromatography of phosphopeptides. Anal Chem 1999;71:2883-2892.
12. Ficarro SB, McCleland ML, Stukenberg PT, Burke DJ, Ross MM, Shabanowitz J, et al. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat Biotechnol 2002;20:301-305.
13. Kinoshita E, Takahashi M, Takeda H, Shiro M, Koike T. Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc(II) complex. Dalton Trans 2004:1189-1193.
14. Kinoshita E, Yamada A, Takeda H, Kinoshita-Kikuta E, Koike T. Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins. J Sep Sci 2005;28:155-162.
15. Nakanishi T, Ohtsu I, Furuta M, Ando E, Nishimura O. Direct MS/MS analysis of proteins blotted on membranes by a matrixassisted laser desorption/ionization-quadrupole ion trap-timeflight tandem mass spectrometry. J Proteome Res 2005;4:743-747.
16. Kinoshita E, Kinoshita-Kikuta E, Takiyama K, Koike T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol Cell Proteomics 2006;5:749-757.
17. Kobayashi T, Nakatani Y, Tanioka T, Tsujimoto M, Nakajo S, Nakaya K, et al. Regulation of cytosolic prostaglandin E synthase by phosphorylation. Biochem J 2004;381:59-69.
18. Hirano H, Watanabe T. Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: Application to an insoluble protein. Electrophoresis 1990;11:573-580.
19. Mansfield MA. Rapid immunodetection on polyvinylidene fluoride membrane blots without blocking. Anal Biochem 1995;229:140-143.
20. Hong HY, Yoo GS., Choi JK. Direct Blue 71 staining of proteins bound to blotting membranes. Electrophoresis 2000;21:841-845.
21. Ohtsu I, Nakanishi T, Furuta M, Ando E, Nishimura O. Direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification of proteins on membrane detected by Western blotting and lectin blotting. J Proteome Res 2005;4:1391-1396.
22. Kinoshita-Kikuta E, Kinoshita E, Yamada A, Endo M, Koike T. Enrichment of phosphorylated proteins from cell lysates using a novel phosphate-affinity chromatography at physiological pH. Proteomics 2006;6:5088-5095.
23. Bodenmiller B, Mueller LN, Mueller M, Domon B, Aebersold R. Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Nat Methods 2007;4:231-237.