Elution mechanism of polypeptides in reversed-phase liquid chromatography based on the critical threshold of organic solvent to induce abrupt change in adsorption capacity to the column packing

Biomedical Chromatography

Volumn 22, Issue 1, 2008, Pages 81-91

  Goda, Ryoya ^a   Sudo, Kenichi ^a  

^a DAIICHI SANKYO CO   (Japan)

Author keywords

Abrupt change in adsorption capacity of polypeptide to column packing; Critical threshold; Power law relationship between retention time and elution rate; Reversed phase liquid chromatography

Indexed keywords

COLUMN CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; ORGANIC SOLVENTS; POLYPEPTIDES;

ABRUPT CHANGE IN ADSORPTION CAPACITY OF POLYPEPTIDE TO COLUMN PACKING; ADSORPTION CAPACITIES; COLUMN PACKINGS; CRITICAL THRESHOLD; ELUENTS; POWER LAW RELATIONSHIP BETWEEN RETENTION TIME AND ELUTION RATE; POWER-LAW RELATIONSHIP; RETENTION TIME; REVERSED PHASE LIQUID-CHROMATOGRAPHY; REVERSED-PHASE LIQUID CHROMATOGRAPHY;

ADSORPTION;

ORGANIC SOLVENT; PEPTIDE DERIVATIVE; POLYPEPTIDE; UROCORTIN;

ADSORPTION; ADSORPTION KINETICS; ARTICLE; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; ELUTION; MATHEMATICAL ANALYSIS; MATHEMATICAL COMPUTING; MOLECULAR MECHANICS; PROTEIN DETERMINATION; REFERENCE VALUE; RESIDUE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY;

EID: 38749119724     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.899     Document Type: Article

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