Highly sensitive and quantitative analysis of polypeptides using a new gradient system based on an abrupt change in adsorption of polypeptide to the reversed-phase column packing

Biomedical Chromatography

Volumn 21, Issue 10, 2007, Pages 1005-1015

  Goda, Ryoya ^a   Sudo, Kenichi ^a  

^a DAIICHI SANKYO CO   (Japan)

Author keywords

A new gradient system; Abrupt change in adsorption capacity of polypeptide; Critical threshold; Highly sensitive and quantitative determination of polypeptides; Reversed phase HPLC

Indexed keywords

ADSORPTION; DICHROISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC SOLVENTS; POLYPEPTIDES;

A NEW GRADIENT SYSTEM; ABRUPT CHANGE IN ADSORPTION CAPACITY OF POLYPEPTIDE; ADSORPTION CAPACITIES; COLUMN PACKINGS; CONFORMATIONAL CHANGE; CRITICAL THRESHOLD; GRADIENT SYSTEMS; HIGHLY SENSITIVE AND QUANTITATIVE DETERMINATION OF POLYPEPTIDE; QUANTITATIVE DETERMINATIONS; REVERSED PHASE HPLC;

ACETONITRILE;

ACETONITRILE; AMYLOID BETA PROTEIN[1-40]; BRAIN NATRIURETIC PEPTIDE; BRAIN NATRIURETIC PEPTIDE 32; GROWTH HORMONE RELEASING FACTOR; INSULIN; ISOLEUCYLSERYLBRADYKININ; MIDKINE; NATRIURETIC PEPTIDE TYPE C; NATRIURETIC PEPTIDE TYPE C 53; NEUROMEDIN C; NEUROPEPTIDE Y; NOCICEPTIN; OXYTOCIN; PEPTIDE; POLYPEPTIDE; UNCLASSIFIED DRUG; UROCORTIN;

ADSORPTION CHROMATOGRAPHY; ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; HUMAN; MOLECULAR WEIGHT; NONHUMAN; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS;

EID: 34848876025     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.825     Document Type: Article

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