Using inhibitors to investigate the involvement of cell signaling in predation by marine phagotrophic protists

Journal of Eukaryotic Microbiology

Volumn 55, Issue 1, 2008, Pages 18-21

  Hartz, Aaron J ^a   Sherr, Barry F ^a   Sherr, Evelyn B ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

Cell signaling; Chemosensory response; Heterotrophic protist; Oxyrrhis marina; Prey ingestion; Uronema sp.

Indexed keywords

G PROTEIN COUPLED RECEPTOR; PROTEIN KINASE INHIBITOR; SEA WATER;

CHEMORECEPTION; CILIATE; CYTOLOGY; DINOFLAGELLATE; ENZYME ACTIVITY; INHIBITION; INHIBITOR; PHAGOTROPHY; PREDATION; PROTIST; SIGNALING;

ANIMAL; APOPTOSIS; ARTICLE; CHEMOTAXIS; CILIATE; DINOFLAGELLATE; DRUG ANTAGONISM; DRUG EFFECT; PARASITOLOGY; PHAGOCYTOSIS; PHYSIOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; APOPTOSIS; CHEMOTAXIS; CILIOPHORA; DINOFLAGELLIDA; PHAGOCYTOSIS; PROTEIN KINASE INHIBITORS; RECEPTORS, G-PROTEIN-COUPLED; SEAWATER; SIGNAL TRANSDUCTION;

CILIOPHORA; DINOPHYCEAE; EUKARYOTA; OXYRRHIS MARINA; PROTISTA; URONEMA (CILIATA);

EID: 38649123685     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2007.00297.x     Document Type: Article

References (32)

1. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. Walter, P. 2002. Molecular Biology of the Cell. 4th ed. Garland Science, New York.
2. Boenigk, J. Arndt, H. 2000a. Comparative studies on the feeding behavior of two heterotrophic nanoflagellates: the filter-feeding choanoflagellate Monosiga ovata and the raptorial-feeding kinetoplastid Rhynchomonas nasuta. Aquat. Microb. Ecol., 22 : 243 249.
3. Boenigk, J. Arndt, H. 2000b. Particle handling during interception feeding of four species of heterotrophic nanoflagellates. J. Eukaryot. Microbiol., 47 : 350 358.
4. Das, M., Mukherjee, S. Shaha, C. 2001. Hydrogen peroxide induces apoptosis-like death in Leishmania donovani promastigotes. J. Cell Sci., 114 : 2461 2469.
5. Devreotes, P. Janetopoulos, C. 2003. Eukaryotic chemotaxis: distinctions between directional sensing and polarization. J. Biol. Chem., 278 : 20445 20448.
6. Elliott, J., Scarpello, J. Morgan, N. 2002. Differential effects of genistein on apoptosis induced by fluoride and pertussis toxin in human and rat pancreatic islets and RINm5F cells. J. Endocrinol., 172 : 137 143.
7. Fenchel, T. 1987. Ecology of Protozoa: The Biology of Free-Living Phagotrophic Protists. Science Tech., Springer, Berlin. 197 p.
8. Fenchel, T. Blackburn, N. 1999. Motile chemosensory behavior of phagotrophic protists: mechanisms for and efficiency in congregating at food patches. Protist, 150 : 325 336.
9. Fenchel, T. Jonsson, P. 1988. The functional biology of Strombidium sulcatum, a marine oligotrich ciliate (Ciliophora, Oligotrichina). Mar. Ecol. Prog. Ser., 48 : 1 15.
10. Gomperts, B., Kramer, I. Tatham, P. 2004. Signal Transduction. Elsevier Academic Press, Amsterdam. 424 p.
11. Haribabu, B., Zhelez, D., Pridgen, B., Richardson, R., Ali, H. Snyderman, R. 1999. Chemoattractant receptors activate distinct pathways for chemotaxis and secretion. J. Biol. Chem., 274 : 37087 37092.
12. Hunter, T. 2000. Signaling - 2000 and beyond. Cell, 1000 : 113 127.
13. King, N. Carroll, S. 2001. A receptor tyrosine kinase from choanoflagellates: molecular insights into early animal evolution. Proc. Natl. Acad. Sci. USA, 98 : 15032 15037.
14. King, N., Hittinger, C. Caroll, S. 2003. Evolution of key signaling and adhesion protein families predates animal origins. Science, 301 : 361 363.
15. Leick, V., Iversen, C., Kemp, K. Christensen, S. 1997. Protein kinase inhibitors abolish adaptative cell behaviour in Tetrahymena. Acta Protozool., 36 : 249 260.
16. Manahan, C., Iglesias, P., Long, Y. Devreotes, P. 2004. Chemoattractant signaling in Dictyostelium discoideum. Ann. Rev. Cell Dev. Biol., 20 : 223 253.
17. Marie, D., Partensky, F., Jacquet, S. Vaulot, D. 1997. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Env. Microbiol., 63 : 186 193.
18. Rasmussen, L., Christensen, S., Schousboe, P. Wheatley, D. 1996. Cell survival and multiplication. The overriding need for signals: from unicellular to multicellular systems. FEMS Microbiol. Lett., 137 : 123 128.
19. Renaud, F., De Ondarza, J., Luporini, P., Marino, M. Van Houten, J. 2004. Signal transduction mechanisms in protozoa. In : Fairweather, I. (ed.), Cell Signaling in Prokaryotes and in Lower Metazoa. Kluwer Academic Publishers, Great Britain. p. 73 90.
20. Rosales, C. 2005. Molecular Mechanisms of Phagocytosis. Landes Biosciences, Springer, Berlin. 154 p.
21. Sherr, E., Caron, D. Sherr, B. 1993. Staining of heterotrophic protists for visualization via epifluorescence microscopy. In : Kemp, P., Sherr, B., Sherr, E. Cole, J. (ed.), Handbook of Methods in Aquatic Microbial Ecology. Lewis Publishers, Boca Raton. p. 213 227.
22. Sherr, E. B., Sherr, B. F. Longnecker, K. 2006. Distribution of bacterial abundance and cell-specific nucleic acid content in the Northeast Pacific Ocean. Deep-Sea Res. I, 53 : 713 725.
23. Sibbald, M. J., Albright, L. Sibbald, P. 1987. Chemosensory responses of a heterotrophic microflagellate to bacteria and several nitrogen compounds. Mar. Ecol. Prog. Ser., 36 : 201 204.
24. Sjoblad, R., Chet, I. Mitchell, R. 1978. Quantitative assay for algal chemotaxis. Appl. Env. Microbiol., 36 : 847 850.
25. Strom, S. 2000. Bacterivory: interactions between bacteria and their grazers. In : Kirchman, D. L. (ed.), Microbial Ecology of the Oceans. Wiley-Liss, New York.
26. Strom, S., Wolfe, G. Bright, K. 2007. Inhibition of marine planktonic protists by amino acids. I. Feeding and behavior in the ciliate Favella sp. Aquat Microbiol. Ecol., 47 : 107 121.
27. 2+ is not required for chemotaxis in Dictyostelium. EMBO, 19 : 4846 4854.
28. Van Engeland, M., Nieland, L., Ramaekers, F., Schutte, B. Reutelingsperger, C. 1998. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry, 31 : 1 9.
29. Van Haastert, P. Devreotes, P. 2004. Chemotaxis: signaling the way forward. Nat. Rev. Mol. Cell Biol., 5 : 626 634.
30. Verity, P. 1991. Feeding of planktonic protozoans: evidence for non-random acquisition of prey. J. Protozool., 38 : 69 76.
31. Wolfe, G. V. 2000. The chemical defense ecology of marine unicellular plankton: constraints, mechanisms, and impacts. Biol. Bull., 198 : 225 244.
32. Wootton, E., Zubkov, M., Jones, D., Jones, R., Martel, C., Thornton, C. Roberts, E. 2007. Biochemical prey recognition by planktonic protozoa. Environ. Microbiol., 9 : 216 222.