Detection of Helicobacter pylori gastritis by PCR : Correlation with inflammation scores and immunohistochemical and CLOtest findings

American Journal of Clinical Pathology

Volumn 129, Issue 1, 2008, Pages 89-96

  Weiss, Jason ^a,b   Tsang, Tai Kin ^b,c   Meng, Xiangwen ^d   Zhang, H ^d   Kilner, Elizabeth ^a   Wang, Edward ^e   Watkin, William ^a,b,f  

^a Laboratory Medicine   (United States)

^b School of Medicine ^*  (United States)

^c Evanston Northwestern Healthcare   (United States)

^d Research Institute   (United States)

^e NORTHWESTERN UNIVERSITY   (United States)

^f NONE   (United States)

Author keywords

Endoscopic biopsies; Gastritis; Helicobacter pylori; Histology; Immunohistochemistry; Polymerase chain reaction; Sydney system

Indexed keywords

ADULT; AGED; ARTICLE; BACTERIUM DETECTION; DYSPEPSIA; ESOPHAGOGASTRODUODENOSCOPY; FEMALE; GASTRITIS; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INFLAMMATION; LOWER ESOPHAGUS SPHINCTER; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STOMACH BIOPSY; ADOLESCENT; BIOPSY; COMPARATIVE STUDY; DIGESTIVE TRACT ENDOSCOPY; GENETICS; HELICOBACTER INFECTION; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; UPPER GASTROINTESTINAL TRACT;

BACTERIAL DNA;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BIOPSY; DNA, BACTERIAL; DYSPEPSIA; ENDOSCOPY, DIGESTIVE SYSTEM; FEMALE; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; UPPER GASTROINTESTINAL TRACT;

EID: 38449118493     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1309/APMPEP54G7PN958G     Document Type: Article

References (26)

1. Das S, Suarez G, Beswick EJ, et al. Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection. J Immunol. 2006;176:3000-3009.
2. Zsikla V, Hailemariam S, Baumann M, et al. Increased rate of Helicobacter pylori infection detected by PCR in biopsies with chronic gastritis. Am J Surg Pathol. 2006;30:242-248.
3. Asrat D, Nilsson I, Mengistu Y, et al. Prevalence of Helicobacter pylori infection among adult dyspeptic patients in Ethiopia. Ann Trop Med Parasitol. 2004;98:181-189.
4. Go MF. Natural history and epidemiology of Helicobacter pylori infection. Aliment Pharmacol Ther. 2002;16(suppl 1):3-15.
5. Hooton C, Keohane J, Clair J, et al. Comparison of three stool antigen assays with the 13C-urea breath test for the primary diagnosis of Helicobacter pylori infection and monitoring treatment outcome. Eur J Gastroenterol Hepatol. 2006;18:595-599.
6. Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin Microbiol Rev. 1997;10:720-741.
7. Raguza D, Granato C F, Kawakami E. Evaluation of the stool antigen test for Helicobacter pylori in children and adolescents. Dig Dis Sci. 2005;50:453-457.
8. Dixon M F, Genta RM, Yardley JH, et al. Classification and grading of gastritis: the updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol. 1996;20:1161-1181.
9. Chan WY, Hui PK, Leung KM, et al. Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol. 1994;102:503-507.
10. Ashton-Key M, Diss TC, Isaacson PG. Detection of Helicobacter pylori in gastric biopsy and resection specimens. J Clin Pathol. 1996;49:107-111.
11. Kabir S. Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. J Med Microbiol. 2001;50:1021-1029.
12. Goosen C, Theron J, Ntsala M, et al. Evaluation of a novel heminested PCR assay based on the phosphoglucosamine mutase gene for detection of Helicobacter pylori in saliva and dental plaque. J Clin Microbiol. 2002;40:205-209.
13. Ferguson DA Jr, Li C, Patel NR, et al. Isolation of Helicobacter pylori from saliva. J Clin Microbiol. 1993;31:2802-2804.
14. Sambrook J, Russell D W. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001.
15. Lin TT, Yeh CT, Wu CS, et al. Detection and partial sequence analysis of Helicobacter pylori DNA in the bile samples. Dig Dis Sci. 1995;40:2214-2219.
16. Pacheco N, Mago V, Gomez I, et al. Comparison of PCR and common clinical tests for the diagnosis of H pylori in dyspeptic patients. Diagn Microbiol Infect Dis. 2001;39:207-210.
17. Nilsson HO, Taneera J, Castedal M, et al. Identification of Helicobacter pylori and other Helicobacter species by PCR, hybridization, and partial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. J Clin Microbiol. 2000;38:1072-1076.
18. He Q, Wang J P, Osato M, et al. Real-time quantitative PCR for detection of Helicobacter pylori. J Clin Microbiol. 2002;40:3720-3728.
19. Aydin O, Egilmez R, Karabacak T, et al. Interobserver variation in histopathological assessment of Helicobacter pylori gastritis. World J Gastroenterol. 2003;9:2232-2235.
20. Meng X, Zhang H, Tsang T. Comparison of a novel multiplex PCR and histology method for the diagnosis of Helicobacter pylori [abstract]. Gastroenterology. 2004;126:A187.
21. Meng X, Zhang H, Tsang T, et al. Detection of Helicobacter pylori by a novel multiplex PCR assay [abstract]. Gastroenterology. 2003;124:A175.
22. Lascols C, Lamarque D, Costa JM, et al. Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H pylori isolates from gastric biopsy specimens by real-time PCR. J Clin Microbiol. 2003;41:4573-4577.
23. Genta RM, Graham DY. Comparison of biopsy sites for the histopathologic diagnosis of Helicobacter pylori: a topographic study of H pylori density and distribution. Gastrointest Endosc. 1994;40:342-345.
24. Gallo N, Zambon CF, Navaglia F, et al. Helicobacter pylori infection in children and adults: a single pathogen but a different pathology. Helicobacter. 2003;8:21-28.
25. Yamamura F, Yoshikawa N, Akita Y, et al. Relationship between Helicobacter pylori infection and histologic features of gastritis in biopsy specimens in gastroduodenal diseases, including evaluation of diagnosis by polymerase chain reaction assay. J Gastroenterol. 1999;34:461-466.
26. Furuta T, Kaneko E, Suzuki M, et al. Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments. J Clin Microbiol. 1996;34:2421-2425.