Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability

Journal of Biochemistry

Volumn 141, Issue 4, 2007, Pages 553-562

  Zhang, Zilian ^a,b   Akutsu, Jun Ichi ^a   Tsujimura, Masanari ^a,b   Kawarabayasi, Yutaka ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b PSS Co Ltd   (Japan)

Author keywords

GlcNAc 1 phosphate uridyltransferase; Improvement of enzymatic activity; Targeted mutagenesis; Thermostable enzyme; UDP GlcNAc

Indexed keywords

HEXOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; MUTANT PROTEIN; N ACETYL D GLUCOSAMINE 1 PHOSPHATE URIDYLTRANSFERASE; PROTEIN ST0452; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE; ARCHAEAL PROTEIN; METAL; NUCLEOTIDYLTRANSFERASE; UDPACETYLGLUCOSAMINE PYROPHOSPHORYLASE;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; IN VITRO STUDY; MUTAGENESIS; NONHUMAN; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; STREPTOCOCCUS PNEUMONIAE; SULFOLOBUS; SULFOLOBUS TOKADAII; THERMOSTABILITY; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS;

ARCHAEA; SULFOLOBUS TOKODAII STR. 7;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; ENZYME STABILITY; HEAT; METALS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEOTIDYLTRANSFERASES; SEQUENCE ALIGNMENT; SULFOLOBUS;

EID: 38449095658     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvm058     Document Type: Article

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