Crystal Structure of Streptococcus pneumoniae N-Acetyl-glucosamine-1-phosphate Uridyltransferase Bound to Acetyl-coenzyme A Reveals a Novel Active Site Architecture

Journal of Biological Chemistry

Volumn 276, Issue 15, 2001, Pages 11844-11851

  Sulzenbacher, Gerlind ^a   Gal, Laurent ^a,c   Peneff, Caroline ^a   Fassy, Florence ^b   Bourne, Yves ^a  

^a Centre National de la Recherche Scientifique   (France)

^b SANOFI   (France)

^c UNIVERSITÉ DE BOURGOGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BACTERIA; BIOSYNTHESIS; CATALYSIS; CELLS; COENZYMES; CRYSTAL STRUCTURE;

ANTIBACTERIAL AGENTS; BIOSYNTHETIC PATHWAYS;

BIOCHEMISTRY;

ACETYL COENZYME A; ANTIBIOTIC AGENT; ANTIINFECTIVE AGENT; BACTERIAL ENZYME; BETA LACTAM ANTIBIOTIC; GLYCOPEPTIDE; N ACETYLGLUCOSAMINE 1 PHOSPHATE URIDYLTRANSFERASE; UNCLASSIFIED DRUG; NUCLEOTIDYLTRANSFERASE; UDPACETYLGLUCOSAMINE PYROPHOSPHORYLASE;

ALPHA HELIX; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL WALL; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; DRUG DESIGN; DRUG TARGETING; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME MECHANISM; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; STREPTOCOCCUS PNEUMONIAE; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; MOLECULAR GENETICS; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE;

ACETYL COENZYME A; AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEOTIDYLTRANSFERASES; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0035853790     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M011225200     Document Type: Article

References (28)

1. Mengin-Lecreulx, D., and van Heijenoort, J. J. (1993) J. Bacteriol. 175, 6150-6157
2. Raetz, C. R. H. (1996) in Escherichia coli and Salmonella: Cellular and Molecular Biology (Neidhardt, F. C., Lin, E., Low, K. B., Magasanik, B., Reznikoff, W. S., Riley, M., Schaechter, M., and Umbarger, H. E., eds) 2nd Ed., Vol. 10, pp. 1035-1063, ASM Press, Washington, D. C.
3. Brown, K., Pompeo, F., Dixon, S., Mengin-Lecreulx, D., Cambillau, C., and Bourne, Y. (1999) EMBO J. 18, 4096-4107
4. Gehring, A. M., Lees, W. J., Mindiola, D. J., Walsh, C. T., and Brown, E. D. (1996) Biochemistry 35, 579-585
5. Mengin-Lecreulx, D., and van Heijenoort, J. (1994) J. Bacteriol. 176, 5788-5795
6. Rossmann, M. G., Liljas, A., Branden, C.-I., and Bansazak, L. J. (1975) in The Enzymes (Boyer, P. D., ed) Vol. 11, pp. 61-102, Academic Press, New York
7. Vuorio, R., Hirvas, L., and Vaara, M. (1991) FEBS Lett. 292, 90-94
8. Van Duyne, G. D., Standaert, R. F., Karplus, P. A., Schreiber, S. L., and Clardy, J. (1993) J. Mol. Biol. 229, 105-124
9. Otwinowski, Z., and Minor, W. (1997) in Methods in Enzymology (Carter, J. C. W., and Sweet, R. M., eds) pp. 307-326, Academic Press, New York
10. CCP4 (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 760
11. Terwilliger, T. C., and Berendzen, J. (1999) Acta Crystallogr. Sect. D Biol. Crystallogr. 55, 849-861
12. Cowtan, K. (1994) Joint CCP4 and ESF-EACMB Newsletter on Protein Crystallography 31, 34-38
13. Roussel, A., and Cambillau, C. (1991) in Silicon Graphics Geometry Partners Directory, pp. 81, Silicon Graphics Corp., Mountain View, CA
14. Navaza, J. (1994) Acta Crystallogr. Sect. A 50, 157-163
15. Murshudov, G. N., Vagin, A. A., and Dodson, E. J. (1997) Acta Crystallogr. Sect. D Biol. Crystallogr. 53, 240-255
16. Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
17. Perrakis, A., Morris, R., and Lamzin, V. S. (1999) Nat. Struct. Biol. 6, 458-463
18. Laskowski, R., MacArthur, M., Moss, D., and Thornton, J. (1993) Appl. Crystallogr. 26, 91-97
19. Barton, G. J. (1993) Protein Eng. 6, 37-40
20. Christopher, J. A. (1998) SPOCK. The Center for Macromolecular Design, Texas A&M University, College Station, TX
21. Merritt, E. A., and Murphy, M. E. P. (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 869-873
22. Sheu, K. F., and Frey, P. A. (1978) J. Biol. Chem. 253, 3378-3780
23. Schlunegger, M. P., Bennett, M. J., and Eisenberg, D. (1997) Adv. Protein Chem. 50, 61-122
24. Beaman, T. W., Blanchard, J. S., and Roderick, S. L. (1998) Biochemistry 37, 10363-10369
25. Cheon, H. G., and Hanna, P. E. (1992) Biochem. Pharmacol. 43, 2255-2268
26. De Angelis, J., Gastel, J., Klein, D. C., and Cole, P. A. (1998) J. Biol. Chem. 273, 3045-3050
27. Pompeo, F., van Heijenoort, J., and Mengin-Lecreulx, D. (1998) J. Bacteriol. 180, 4799-4803
28. Beaman, T. W., Sugantino, M., and Roderick, S. L. (1998) Biochemistry 37, 6689-6696