Few modifications of the Cobas Amplicor HIV Monitor 1.5 test allow reliable quantitation of HIV-1 proviral load in peripheral blood mononuclear cells

Journal of Virological Methods

Volumn 120, Issue 2, 2004, Pages 201-205

  Lillo, Flavia B ^a   Grasso, Maria Angela ^a   Lodini, Sara ^a   Bellotti, Maria Grazia ^c   Colucci, Giuseppe ^b  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b Roche Molecular Systems   (Switzerland)

^c Roche Diagnostics Systems SpA   (Italy)

Author keywords

CAHIM; HAART monitoring; HIV DNA

Indexed keywords

CD4 ANTIGEN; VIRUS DNA;

ANALYTIC METHOD; ARTICLE; CELL COUNT; CONTROLLED STUDY; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; LINEAR SYSTEM; MAJOR CLINICAL STUDY; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SENSITIVITY AND SPECIFICITY; VIREMIA; VIRUS DETECTION; VIRUS LOAD; VIRUS TITRATION;

ANTIRETROVIRAL THERAPY, HIGHLY ACTIVE; CHILD, PRESCHOOL; DNA, VIRAL; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; INFANT; INFANT, NEWBORN; LEUKOCYTES, MONONUCLEAR; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PROVIRUSES; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; VIRAL LOAD;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 3843151525     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.05.008     Document Type: Article

References (16)

1. Borkowsky W., Krasinski K., Pollack H., Hoover W., Kaul A., Ilmet-Moore T. Early diagnosis of human immunodeficiency virus infection in children less than 6 months of age: comparison of polymerase chain reaction, culture, and plasma antigen capture techniques. J. Infect. Dis. 166:1992;616-619
2. Christopherson C., Kidane Y., Conway B., Krowka J., Sheppard H., Kwok S. PCR-based assay to quantify of human immunodeficiency type 1 DNA in pheripheral blood mononuclear cells. J. Clin. Microbiol. 38(2):2000;630-634
3. Chun T.W., Justement S., Pandya P., Hallahan C.W., McLoughlin M., Liu S., Ehler L.A., Kovacs C., Fauci A.S. Relationship between the size of human immunodeficiency virus type 1 (HIV-1) reservoir in peripheral blood CD4+ T cells and CD4+:CD8+ T cell ratios in aviremic HIV-1-infected individuals receiving long-term highly active antiretroviral therapy. JID. 185:2002;1672-1676
4. Chun T.W., Stuyver L., Mizell S.B., Ehler L.A., Mican J.A., Baseler M., Lloyd A.L., Nowak M.A., Fauci A.S. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl. Acad. Sci. U.S.A. 94:1997;13193-13197
5. Donadula G., Nunnari G., Vanella M., Roman J., Babinchack T., DeSimone J., Stern J., Braffman M., Zhang H., Pomerantz R.J. Human immunodeficiency virus type 1-infected persons with residual disease and virus reservoirs on suppressive highly active antiretroviral therapy can be stratified into relevant virologic and immunologic subgroups. JID. 183:2001;1682-1687
6. Finzi D., Hermankova M., Pierson T., Carruth L.M., Buck C., Chaisson R.E., Quinn T.C., Chardwick K., Margolick J., Brookmayer R., Gallant J., Markowitz M., Ho D.D., Richman D.D., Siliciano R.F. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science. 278:1997;1295-1300
7. Folks, T.M., Powell, D., Lightfoote, M., Koenig, S., Fauci, A.S., Benn, S., Rabson, A., Daugherty, D., Gendelman, H.E., Hoggan M.D., 1986. Biological and biochemical characterization of a cloned Leu 3-cell surviving infection with the acquired immunodeficiency syndrome retro virus. J. Exp. Med. 164, 280-290.
8. Garrigue I., Pellegrin I., Hoen B., Dumon B., Harzic M., Schrive M.H., Sereni D., Fleury H. Cell associated HIV-1 DNA quantification after HAART-treated primary infection in patients with persistently undetectable plasma HIV-RNA. AIDS. 14:2000;2851-2855
9. Gupta, P.M., Ding, M., Cottril, M., Rinaldo, C., Kingsley, L., Wolinsky, S., Mellors, J., 1995. Quantitation of human immunodeficiency type 1 DNA and RNA by a novel internally controlled PCR assay. J. Clin. Microbiol. 33, 1670-1673.
10. Ho D.D., Neuman A.U., Perelson A.S., Chen W., Leonard J.M., Markowitz M. Rapid turnover of plasma virions and CD4 lymphpcytes in HIV infection. Nature. 373:1995;123
11. Mc Dermott J.L., Giri A.A., Martini I., Bono M., Giacomini M., Campelli A., Tagliaferro L., Cara A., Varnier O.E. Level of human immunodeficiency virus DNA in pheripheral blood mononuclear cells correlates with the efficacy of antiretroviral therapy. J. Clin. Microbiol. 31:1999;2361-2365
12. Ngo-Giang Huong N., Deveau C., Da Silva I., Pellegrin I., Venet A., Harzic M., Sinet M., Delfraissy J.F., Meyer L., Goujard C., Rouzioux C. Proviral HIV-DNA in subjects followed since primary HIV-1 infection who suppress plasma viral load after years of highly active antiretroviral therapy. French PRIMO Cohort Study Group. AIDS. 15:2001;665-673
13. Pellegrin L., Caumont A., Garrigue I., Merel P., Schrive M.E., Fleury H., Dupon M., Pellegrin J.L., Ragnaud J.M. Predictive value of provirus load and human immunodeficiency virus genotype for successful abacavir-based simplified therapy. JID. 187(January):2003;38-46
14. Perelson A.S., Essunger P., Cao Y., Vesanem M., Hurley A., Saksela K., Markowitz M., Ho D. Decay characteristics of HIV-1 infected compartments during combination therapy. Nature. 387:1997;188
15. Wong J.K., Hezareh M., Gunthard H.F., Havlir D.V., Ignacio C.C., Spina C.A., Richman D.D. Recovery of replication competent HIV despite prolonged suppression of plasma viremia. Science. 278:1997;1291-1295
16. Zhao Y., Yu M., Miller J.W., Chen M., Bremer E.G., Kabat W., Yogev R. Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology. JCM. 40(2):2002;675-678