Maintenance of Epstein-Barr virus-derived episomal vectors in the murine Sp2/0 myeloma cell line is dependent upon exogenous expression of human EBP2

Biochemistry and Cell Biology

Volumn 82, Issue 3, 2004, Pages 375-380

  Habel, Marie Eve ^a,b   Drouin, Mathieu ^a   Jung, Daniel ^a,b  

^a HÉMA QUÉBEC   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

EBNA 1; EBP2; EBV vector; Episomal maintenance; Mouse cell; Sp2 0 myeloma cell line

Indexed keywords

BIOMEDICAL ENGINEERING; CELLS; GENES; GENETIC ENGINEERING; PROTEINS;

EPISOMES; EPSTEIN-BARR VIRUS (EBV); HUMAN CELLS; ORIGIN OF REPLICATION (ORIP);

VIRUSES;

PROTEIN; PROTEIN EBP2; UNCLASSIFIED DRUG; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA REPLICATION ORIGIN; EPISOME; EPSTEIN BARR VIRUS; GENETIC ENGINEERING; MOUSE; MYELOMA CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY;

ANIMALS; CARRIER PROTEINS; CELL LINE, TUMOR; DNA REPLICATION; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; GENE EXPRESSION; GENETIC VECTORS; HERPESVIRUS 4, HUMAN; HUMANS; MICE; MULTIPLE MYELOMA; PLASMIDS; PROTEIN BINDING; REPLICATION ORIGIN;

ANIMALIA; HUMAN HERPESVIRUS 4; MURINAE;

EID: 3843064604     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o04-037     Document Type: Article

References (28)

1. Bowers, M.T., Ramachandran, S., and Yu, B.W. 1999. Modified miniprep method for the rapid recovery of episomes from transfected breast epithelial cells. Biotechniques. 26: 276-282.
2. Chaudhuri, B., Xu, H., Todorov, I., Dutta, A., and Yates, J.L. 2001. Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. Proc. Natl. Acad. Sci. USA. 98: 10 085-10 089.
3. Dhar, S.K., Yoshida, K., Machida, Y., Khaira, P., Chaudhuri, B., Wohlschlegel, J.A., et al. 2001. Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. Cell, 106: 287-296.
4. Doerfler, W., Schubbert, R., Heller, H., Kammer, C., Hilger-Eversheim, K., Knoblauch, M., and Remus, R. 1997. Integration of foreign DNA and its consequences in mammalian systems. Trends Biotechnol. 15: 297-301.
5. Frappier, L., and O'Donnell, M. 1991. Overproduction, purification, and characterization of EBNA1, the origin binding protein of Epstein-Barr virus. J. Biol. Chem. 266: 7819-7826.
6. Gahn, T.A., and Schildkraut, C.L. 1989. The Epstein-Barr virus origin of plasmid replication, oriP, contains both the initiation and termination sites of DNA replication. Cell, 58: 527-535.
7. Gulley, M.L., Raphael, M., Lutz, C.T., Ross, D.W., and Raab-Traub, N. 1992. Epstein-Barr virus integration in human lymphomas and lymphoid cell lines. Cancer, 70: 185-191.
8. Hearing, J., Mulhaupt, Y., and Harper, S. 1992. Interaction of Epstein-Barr virus nuclear antigen 1 with the viral latent origin of replication. J. Virol. 66: 694-705.
9. Hirt, B. 1967. Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26: 365-369.
10. Huertas, D., Howe, S., McGuigan, A., and Huxley, C. 2000. Expression of the human CFTR gene from episomal oriP-EBNA1-YACs in mouse cells. Hum. Mol Genet. 9: 617-629.
11. Kanda, T., Otter, M., and Wahl, G.M. 2001. Coupling of mitotic chromosome tethering and replication competence in Epstein-Barr virus-based plasmids. Mol. Cell Biol. 21: 3576-3588.
12. Kapoor, P., Shire, K., and Frappier, L. 2001. Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast. EMBO J. 20: 222-230.
13. Kelleher, Z.T., Fu, H., Livanos, E., Wendelburg, B., Gulino, S., and Vos, J.M. 1998. Epstein-Barr-based episomal chromosomes shuttle 100 kb of self-replicating circular human DNA in mouse cells. Nat. Biotechnol. 16: 762-768.
14. Krysan, P.J., and Calos, M.P. 1993. Epstein-Barr virus-based vectors that replicate in rodent cells. Gene, 136: 137-143.
15. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
16. Mizuguchi, H., Hosono, T., and Hayakawa, T. 2000. Long-term replication of Epstein-Barr virus-derived episomal vectors in the rodent cells. FEBS Lett. 472: 173-178.
17. Proulx, C., Boyer, L., St-Amour, I., Bazin, R., and Lemieux, R. 2002. Higher affinity human D MoAb prepared by light-chain shuffling and selected by phage display. Transfusion, 42: 59-65.
18. Saeki, Y., Wataya-Kaneda, M., Tanaka, K., and Kaneda, Y. 1998. Sustained transgene expression in vitro and in vivo using an Epstein-Barr virus replicon vector system combined with HVJ liposomes. Gene Ther. 5: 1031-1037.
19. Schepers, A., Ritzi, M., Bousset, K., Kremmer, E., Yates, J.L., Harwood, J., et al. 2001. Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus. EMBO J. 20: 4588-4602.
20. Shire, K., Ceccarelli, D.F., Avolio-Hunter, T.M., and Frappier, L. 1999. EBP2, a human protein that interacts with sequences of the Epstein-Barr virus nuclear antigen 1 important for plasmid maintenance. J. Virol. 73: 2587-2595.
21. Simard, C., Lemieux, R., and Cote, S. 2001. Urea substitutes toxic formamide as destabilizing agent in nucleic acid hybridizations with RNA probes. Electrophoresis, 22: 2679-2683.
22. Sugden, B., and Warren, N. 1989. A promoter of Epstein-Barr virus that can function during latent infection can be trans-activated by EBNA-1, a viral protein required for viral DNA replication during latent infection. J. Virol. 63: 2644-2649.
23. Sugden, B., Marsh, K., and Yates, J. 1985. A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus. Mol. Cell Biol. 5: 410-413.
24. Summers, H., Barwell, J.A., Pfuetzner, R.A., Edwards, A.M., and Frappier, L. 1996. Cooperative assembly of EBNA1 on the Epstein-Barr virus latent origin of replication. J. Virol. 70: 1228-1231.
25. Wu, H., Ceccarelli, D.F., and Frappier, L. 2000. The DNA segregation mechanism of Epstein-Barr virus nuclear antigen 1. EMBO Rep. 1: 140-144.
26. Wysokenski, D.A., and Yates, J.L. 1989. Multiple EBNA1-binding sites are required to form an EBNA1-dependent enhancer and to activate a minimal replicative origin within oriP of Epstein-Barr virus. J. Virol. 63: 2657-2666.
27. Yates, J.L., and Guan, N. 1991. Epstein-Barr virus-derived plasmids replicate only once per cell cycle and are not amplified after entry into cells. J. Virol. 65: 483-488.
28. Yates, J.L., Warren, N., and Sugden, B. 1985. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature (London), 313: 812-815.