Modified miniprep method for the rapid recovery of episomes from transfected breast epithelial cells

BioTechniques

Volumn 26, Issue 2, 1999, Pages 276-282

  Bowers, Mark T ^a   Ramachandran, Satya ^a   Yu, Bennett W ^a  

^a HENRY FORD HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BREAST EPITHELIUM; EPISOME; ESCHERICHIA COLI; EXPRESSION VECTOR; HUMAN; HUMAN CELL; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

ESCHERICHIA COLI; EUKARYOTA; MINK CELL FOCUS-FORMING VIRUS; PROKARYOTA;

EID: 0032966660     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99262st01     Document Type: Article

References (9)

1. Birnboim, H.C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Chittenden, T., S. Lupton and A.J. Levine. 1989. Functional limits of oriP, the Epstein-Barr virus plasmid origin of replication. J. Virol. 65:3016-3025.
3. Heller, R.A., K. Song, D. Villaret, R. Margolskee, J. Dunne, H. Hayakawa and G.M. Ringold. 1990. Amplified expression of tumor necrosis factor receptor in cells transfected with Epstein-Barr virus shuttle vector cDNA libraries. J. Biol. Chem. 265:5708-5717.
4. Hirt, B. 1967. Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26:365-369.
5. Margolskee, R.F., P. Kavathas and P. Berg. 1988. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8:2837-2847.
6. Murphy, A.J.M., A.L. Kung, R.A. Swirski and R.T. Schimke. 1992. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods: A Companion to Methods Enzymol. 4:111-131.
7. Soule, H.D., T.M. Maloney, S.R. Wolman, W.D. Peterson, R. Brenz, C.M. McGrath, J. Russo, R.J. Pauley, R.F. Jones and S.C. Brooks. 1990. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50:6075-6086.
8. Sugden, B., K. Marsh and J. Yates. 1985. A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus. Mol. Cell. Biol. 5:410-413.
9. Tait, L., H.D. Soule and J. Russo. 1990. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50:6087-6094.