Expression, purification and characterization of recombinant mitochondrial topoisomerase II of kinetoplastid Crithidia fasciculata in High-five insect cells

Protein Expression and Purification

Volumn 58, Issue 1, 2008, Pages 122-131

  Wang, Yuzhen ^a   Tsai, Yu Cheng ^a   Urena Rivera, Esteban ^a   Chen, Junghuei ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Catenation; Decatenation; kDNA; Kinetoplastid topoisomerase II

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING); KINETOPLAST DNA; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CELL LINE; CRITHIDIA FASCICULATA; DNA REPLICATION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MITOCHONDRION;

ANIMALS; CELL LINE; CRITHIDIA FASCICULATA; DNA REPLICATION; DNA TOPOISOMERASES, TYPE II; DNA, KINETOPLAST; GENE EXPRESSION; MITOCHONDRIA; RECOMBINANT PROTEINS;

CRITHIDIA FASCICULATA; HEXAPODA; KINETOPLASTIDA;

EID: 38349157756     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.10.014     Document Type: Article

References (28)

1. Das B.B., Sengupta T., Ganguly A., and Majumder H.K. Topoisomerases of kinetoplastid parasites: why so fascinating. Mol. Microbiol. 62 (2006) 917-927
2. Das A., Dasgupta A., Sengupta T., and Majumder H.K. Topoisomerases of kinetoplastid parasites as potential chemotherapeutic targets. Trends Parasitol. 20 (2004) 381-387
3. Das B.B., Sen N., Dasgupta S.B., Ganguly A., Das R., and Majumder H.K. Topoisomerase research of kinetoplastid parasite Leishmania, with special reference to development of therapeutics. Indian J. Med. Res. 123 (2006) 221-232
4. Cortazar T.M., Coombs G.H., and Walker J. Leishmania panamensis: comparative inhibition of nuclear DNA topoisomerase II enzymes from promastigotes and hman macroquinolones, flavonids and pentamidine. Exp. Parasitol. 116 (2007) 475-482
5. Burden D.A., and Osheroff N. Mechanism of action of eukaryotic topoisomerase II and drugs targeted to the enzyme. Biochim. Biophys. Acta 1400 (1998) 139-154
6. Nitiss J.L. DNA topoisomerases in cancer therapy: using enzymes to generate selective DNA damage. Curr. Opin. Investig. Drugs 10 (2002) 1512-1516
7. Denney W.A., and Baguley B.C. Dual topoisomerase I/II inhibitors in cancer therapy. Curr. Top. Med. Chem. 3 (2003) 339-353
8. Englund P.T., and Shapiro T.A. Selective cleavage of kinetoplast DNA minicircles promoted by antitrypanosomal drugs. Proc. Natl. Acad. Sci. USA 87 (1990) 950-954
9. Bailly C., Dassonneville L., Carrasco C., Lucas D., Kumar A., Boykin D.W., and Wilson W.D. Relationships between topoisomerase II inhibition, sequence-specificity and DNA binding mode of dicationic diphenylfuran derivatives. Anti Cancer Drugs 14 (1999) 47-60
10. Weissig V., Vetro-Widenhouse T.S., and Rowe T.C. Topoisomerase II inhibitors induce cleavage of nuclear and 35-kb plastid DNAs in the malarial parasite Plasmodium falciparum. DNA Cell. Biol. 16 (1997) 1483-1492
11. Simpson L. The mitochondrial genome of kinetoplastid protozoa: genomic organization, transcription, replication, and evolution. Ann. Rev. Microbiol. 41 (1987) 363-382
12. Davila A.MR., and Tyler K.M. Combating kinetoplastid diseases. Kinetoplastid Biol. Dis. 1 (2002) 6-7
13. Zhao C., Papadopoulou B., and Tremblay M.J. Leishmania infantum promotes replication of HIV type 1 in human lymphoid tissue cultured ex vivo by inducing secretion of the proinflammatory cytokines TNF-α and IL-1α. J. Immunol. 172 (2004) 3086-3093
14. Hanke T., Ramiro M.J., Trigueros S., and Larraga V. Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs. Nucleic Acids Res. 31 (2003) 4917-4928
15. Cheesman S.J. The topoisomerases of protozoan parasites 16 (2000) 277-281
16. Douc-Rasy S., Riou J.F., Jean-charles A., and Riou G. ATP-independent DNA topoisomerase II as potential drug target in trypanosomes. Biol. Cell 64 (1988) 145-156
17. Shapiro T.A., and England P.T. The structure and replication of kinetoplast DNA. Ann. Rev. Microbiol. 49 (1995) 117-143
18. Liu B., Liu Y., Motyka S.A., Agbo E.E.C., and Englund P.T. Fellowship of the ring: the replication of kinetoplast DNA. Trends Parasitol. 21 (2005) 363-369
19. Wang Z., and Englund P.T. RNA interference of trypanosome topoisomerase II causes progressive loss of mitochondrial DNA. EMBO J. 20 (2001) 4674-4683
20. Pasion S.G., Hines J.C., Aebersold R., and Ray D.S. Molecular cloning and expression of the gene encoding the kinetoplast-associated type II DNA topoisomerase of Crithidia fascicuata. Mol. Biochem. Parasitol. 50 (1992) 57-68
21. Fragoso S.P., Mattei D., Hines J.C., Ray D., and Goldenberg S. Expression and cellular localization of Trypanosoma cruzi type II topoisomerase. Mol. Biochem. Parasitol. 94 (1998) 197-204
22. Douc-Rasy S., Kayser A., Riou J.F., and Riou G. ATP-independent type II topoisomerase from trypanosomes. Proc. Natl. Acad. Sci. USA 83 (1986) 7152-7156
23. Kulikowicz T., and Shapiro T.A. Distinct genes encode type II topoisomerases for the nucleus and mitochondrion in the protozoan parasite Trypanosoma brucei. J. Biol. Chem. 281 (2006) 3048-3056
24. Vittal V.R., Sekher A., and Carter B.J. Isolation and characterization of two distinct ATP-independent topoisomerase II activities from the trypanosomatid Trypanosoma brucei. Protein Pept. Lett. 8 (2001) 1-11
25. Shapiro T.A., Klein V.A., and Englund P.T. Isolation of kinetoplast DNA. In: Bjornsti M.-A., and Osheroff N. (Eds). Methods in Molecular Biology vol. 94 (1999), Humana Press, Berlin 61-68
26. Perez-Morga D.L., and Englund P.T. Microtechnique for electron microscopy of DNA. Nucleic Acids Res. 21 (1993) 1327-1328
27. Kramlinger V.M., and Hiasa H. The GyrA-box is required for the ability of DNA Gyrase to wrap DNA and catalyze the supercoiling reaction. J. Biol. Chem. 281 (2006) 3738-3742
28. Ismail M.A., Brun R., Wenzler T., Tanious F.A., Wilson W.D., and Boykin D.W. Dicationic biphenyl benzimidazole derivatives as antiprotozal agents. Bioorg. Med. Chem. 12 (2004) 5405-5413