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Volumn 9, Issue 26, 2007, Pages 5369-5372

Total synthesis of munchiwarin, a triprenylated chalcone from Crotalaria medicagenia

Author keywords

[No Author keywords available]

Indexed keywords

CHALCONE DERIVATIVE; MUNCHIWARIN; UNCLASSIFIED DRUG;

EID: 38349128558     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol702187m     Document Type: Article
Times cited : (21)

References (28)
  • 12
    • 0036286419 scopus 로고    scopus 로고
    • Lin, y.-M.; Zhou, Y.; Flavin, M. T.; Zhou, L.-.M.; Nie, W.; Chen, F.-C. Bioorg. Med. Chem. 2002, 10, 2795.
    • Lin, y.-M.; Zhou, Y.; Flavin, M. T.; Zhou, L.-.M.; Nie, W.; Chen, F.-C. Bioorg. Med. Chem. 2002, 10, 2795.
  • 21
    • 38349152060 scopus 로고    scopus 로고
    • Please see the Supporting Information for the isolation procedure
    • Please see the Supporting Information for the isolation procedure.
  • 25
    • 38349120341 scopus 로고    scopus 로고
    • Evalution of ID50 of munchiwarin (1, SYBR Green I-based fluorescence (MSF) assay was used. A 200 μL sample of Asynchronous P. falciparum parasites at 0.5% parasitemia, 1.5% hematocrit, and varying concentration of munchiwarin (1) was incubated at 37°C for 72 h. After the incubation, 100 μL of lysis buffer [20 mM Tris (pH 7.5, 5 mM EDTA, 0.008, w/v) saponin, and 0.08, v/v) Triton X-100] containing SYBR Green I, 1 x final concentration) was added to each well and plates were reincubated for another hour at room temperature. The plates were examined for the relative fluorescence units (RFUs) per well using the FLUOstar, BMG labtechnologies. The 50% inhibitory dose (ID50) was determined with GraphPad Prism software by nonlinear regression analysis of log doseresponse curves. For both assays, micro titer plate wells containing noninfected erythrocytes in the absence of drugs served as negative control, while parasitized erythrocytes in the presenc
    • 50) was determined with GraphPad Prism software by nonlinear regression analysis of log doseresponse curves. For both assays, micro titer plate wells containing noninfected erythrocytes in the absence of drugs served as negative control, while parasitized erythrocytes in the presence of chloroquine (CQ) served as positive control.
  • 27
    • 38349157206 scopus 로고    scopus 로고
    • 50) values represent the concentration of compound required to kill 50% of the fibroblast cells.
    • 50) values represent the concentration of compound required to kill 50% of the fibroblast cells.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.