Prenylated chalcones isolated from Crotalaria genus inhibits in vitro growth of the human malaria parasite Plasmodium falciparum

Bioorganic and Medicinal Chemistry Letters

Volumn 15, Issue 10, 2005, Pages 2453-2455

  Narender, T ^a   Shweta, ^a   Tanvir, K ^a   Srinivasa Rao, M ^b   Srivastava, K ^a   Puri, S K ^a  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

^b UNIVERSITY OF RHODE ISLAND   (United States)

Author keywords

Antimalarial activity; Plasmodium falciparum; Prenylated Chalcone

Indexed keywords

ANTIMALARIAL AGENT; CHALCONE DERIVATIVE; CROTAORIXIN; HERBACEOUS AGENT; UNCLASSIFIED DRUG;

ANTIMALARIAL ACTIVITY; ARTICLE; CONCENTRATION RESPONSE; CROTALARIA; CROTALARIA ORIXENSIS; DRUG ISOLATION; DRUG STRUCTURE; GENETIC STRAIN; GENUS; IN VITRO STUDY; MEDICINAL PLANT; NONHUMAN; PLASMODIUM FALCIPARUM; PRENYLATION; SCHIZONT; STRUCTURE ANALYSIS;

CROTALARIA; PLASMODIUM FALCIPARUM;

EID: 18144375933     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.03.081     Document Type: Article

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15. In vitro antimalarial activity: the in vitro antimalarial activity of compound was assessed by evaluating the minimum inhibitory concentration (MIC) against Plasmodium falciparum by following Rieckman's microassay technique with minor modification. Asynchronous parasites obtained from cultures of P. falciparum (Strain NF-54) were synchronized after 5% sorbital treatment so as to obtain only ring stage parasites. Parasite suspension medium RPMI-1640 at 1-2% parasitimia and 3% hematocrit was dispensed into sterile 96 well plates; and test compounds were serially diluted in duplicate wells so as to obtain final concentrations of 50, 10 and 2 μg/ml. The culture plates were incubated in a candle jar at 37°C for 34-36 h. Thin blood smears from each well were microscopically examined and the concentration, which fully inhibited the maturation of the ring stage parasites in to schizont stage, was recorded as MIC
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