Histo-cytological changes in Pelargonium apices during the cryopreservation process: Effect of the osmotic agent chosen for the preculture step

Acta Horticulturae

Volumn 760, Issue , 2007, Pages 195-202

  Grapin, A ^a   Dorion, N ^a   Verdeil, J L ^b   Escoute, J ^b  

^a CEMAGREF   (France)

^b CIRAD   (France)

Author keywords

Desiccation; Encapsulation dehydration; Microscopy; Sucrose; Sugar

Indexed keywords

PELARGONIUM;

EID: 38349125292     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2007.760.25     Document Type: Conference Paper

References (17)

1. Bachiri, Y., Song, G.Q., Plessis, P., Shoar-Ghaffari, A., Rekab, T. and Morisset, C. 2001. Routine cryopreservation of kiwifruit (Actinidia spp) germplasm by encapsulationdehydration: importance of plant growth regulators. CryoLetters 22: 61-74.
2. Bagniol, S., Engelmann, F. and Michaux-Ferrière, N. 1992. Histo-cytological study of apices from in vitro plantlets of date palm (Phoenix dactylifera L.) during a cryopreservation process. Cryo-letters 13: 405-412.
3. Benson, E.E., Johnston, J., Muthusamy, J. and Harding, K. 2006. Physical and engineering perspectives of in vitro plant cryopreservation. Focus on biotechnology 6: 441-476.
4. Dumet, D., Grapin, A., Bailly, C. and Dorion, N. 2002. Revisiting crucial steps of an encapsulation/desiccation based cryopreservation process: Importance of thawing method in the case of Pelargonium meristems. Plant Science 163: 1121-1127.
5. Fabre, J. and Dereuddre, J. 1990. Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot-tips. Cryo-Letters 11: 413-426.
6. Fisher, D.B. 1968. Protein staining of ribboned epon sections for light microscopy. Histochemie 16: 92-96.
7. Gabe, M. 1968. Techniques histologiques. Masson, Paris.
8. Grapin, A., Dumet, D., Holota, H. and Dorion, N. 2003. Cryopreservation of Pelargonium shoot tips by encapsulation-dehydration: Effects of sucrose concentration, dehydration duration, and genotype. Acta Hort.623: 225-230.
9. Grapin A., Hupel C., Marche, C. and Dorion, N. 2001. First results in Pelargonium shoot apices cryopreservation. Acta Hort. 560 157-160.
10. Helliot, B., Panis, B., Poumay, Y., Swennen, R., Lepoivre, P. and Frison, E. 2002. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep. 20: 1117-1122.
11. Hoekstra, F.A., Golovina, E.A., Tetteroo, F.A.A. and Wolkers, W.F. 2001 Induction of desiccation tolerance in plant somatic embryos: How exclusive is the protective role of sugars? Cryobiology 43: 140-150.
12. Morel, G. and Wetmore, R.H. 1951. Fern callus tissue culture. Ann. J. Bot. 38:141-143.
13. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
14. Panis, B., Strosse, H., Van Den Hende, S. and Swennen, R. 2002. Sucrose preculture to simplify cryopreservation of banana meristem cultures. CryoLetters 23:375-384.
15. Paul, H., Daigny, G. and Sangwan-Norreel, B.S. 2000. Cryopreservation of apple (Malus × domestica Borkh.) shoot tips following encapsulation- dehydration or encapsulationvitrification. Plant Cell Rep. 19: 768-774.
16. Sherlock, G., Block, W. and Benson, E.E. 2005. Thermal analysis of the plant encapsulation-dehydration cryopreservation protocol using silica gel as the desiccant CryoLetters 26: 45-54.
17. Sokal, R.R. and Rohlf, F.J. 1981. Tests of independence. p. 731-747. In: WH. Freeman and Company (eds.), Biometry: the principles and practice of statistics in biological research. 2nd Ed. San Francisco.